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Cck 8 assay solution

Manufactured by Yeasen
Sourced in China

The CCK-8 assay solution is a colorimetric cell viability and cytotoxicity detection reagent. It is used to measure the number of viable cells in proliferation or cytotoxicity assays.

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2 protocols using cck 8 assay solution

1

Cell Viability Assay with Wnt-C59 and Microglia

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For determining the cell viability of IDH1WT GL261 cells, IDH1WT CT-2A cells, IDH1WT U251 cells or primary microglia cells after treated with Wnt-C59, the cells were seeded in 96-well plates and then cultured for 1 day, subsequently, exposed into completed DMEM media containing different concentrations of Wnt-C59 (0, 0.05, 0.1, 0.2, 0.4, 0.8, 1.6, 3.2, 6.4 µmol/L). Cancer cell viabilities were determined after culturing with media isolated from primary microglia, immunosuppressive phenotype microglia or Wnt-C59 (3 or 6 µmol/L) treated immunosuppressive microglia (the cells were cultured with Wnt-C59 for 1 day, then washed twice. The cells were cultured for another day in fresh medium). CCK-8 assay solution (10 μL/well, Yeasen Biotechnology) was added and the optical density was obtained after 2 h.
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2

Donepezil Neuroprotection Evaluated

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The HBMECs treated with donepezil (20, 50, 100 μM) and OGD/R were grown in 96-well plates for 24 h incubation. Then, the CCK-8 assay solution (Yeasen, Shanghai, China) was added into the wells for 4 h incubation as recommended by the manufacturer. The optical density (OD = 450 nm) was checked by a microplate reader (Reagen, Shenzhen, China) [24 (link)].
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