Cck 8 assay solution

For determining the cell viability of IDH1^WT GL261 cells, IDH1^WT CT-2A cells, IDH1^WT U251 cells or primary microglia cells after treated with Wnt-C59, the cells were seeded in 96-well plates and then cultured for 1 day, subsequently, exposed into completed DMEM media containing different concentrations of Wnt-C59 (0, 0.05, 0.1, 0.2, 0.4, 0.8, 1.6, 3.2, 6.4 µmol/L). Cancer cell viabilities were determined after culturing with media isolated from primary microglia, immunosuppressive phenotype microglia or Wnt-C59 (3 or 6 µmol/L) treated immunosuppressive microglia (the cells were cultured with Wnt-C59 for 1 day, then washed twice. The cells were cultured for another day in fresh medium). CCK-8 assay solution (10 μL/well, Yeasen Biotechnology) was added and the optical density was obtained after 2 h.