Lutein

Manufactured by ITW Reagents

Sourced in Germany

Lutein is a carotenoid compound used in various laboratory applications. It is a naturally occurring pigment found in plants and certain animal tissues. Lutein has the ability to absorb specific wavelengths of light, making it a useful tool for various analytical and research purposes.

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Lab products found in correlation

α tocopherol, by Merck Group (1 mentions) Porcine bile extract b8631, by Merck Group (1 mentions) Pepsin from porcine gastric mucosa p6887, by Merck Group (1 mentions) β carotene, by Merck Group (1 mentions) Lutein, by Merck Group (1 mentions) Hplc grade, by Merck Group (1 mentions)

2 protocols using lutein

Carotenoid and Vitamin E Extraction

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All solvents were HPLC grade and purchased from Fisher Scientific (Loughborough, UK). Standards (β-carotene and α-tocopherol) were HPLC grade and purchased from Sigma Aldrich (Merck, Darmstadt, Germany), except lutein which was purchased from PanReac AppliChem (Darmstadt, Germany). Enzymes, including α-amylase from human saliva type XIII-A, 300-1500 U mg -1 protein (A1031), pepsin from porcine gastric mucosa (P6887), pancreatin from porcine pancreas (8xUSP; P7545), and porcine bile extract (B8631), were purchased from Sigma Aldrich (Merck, Darmstadt, Germany). Other chemicals used were of analytical grade.

Wattanakul J., Syamila M., Darwish R., Gedi M.A., Sutcharit P., Chi C., Akepach P., Sahaka M., Gontero B., Carrière F, & Gray D.A. (2022). Bioaccessibility of essential lipophilic nutrients in a chloroplast-rich fraction (CRF) from agricultural green waste during simulated human gastrointestinal tract digestion. Food & function, 13(9).

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Lipid Extraction and Analysis Protocol

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Acetone, acetonitrile, chloroform, hexane and methanol used for lipid extraction and analysis of β-carotene, lutein, and α-tocopherol, were HPLC grade, purchased from Merck (Darmstadt, Germany) and VWR International (Lutterworth, England). Butylated hydroxytoluene (BHT) and standards of β-carotene, and αtocopherol were purchased from Merck (Darmstadt, Germany) and lutein from Pan Reac AppliChem (Barcelona).

Syamila M., Gedi M.A., Briars R., Ayed C, & Gray D.A. (2019). Effect of temperature, oxygen and light on the degradation of β-carotene, lutein and α-tocopherol in spray-dried spinach juice powder during storage. Food chemistry, 284.

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