Spectrophotometer uv vis seri uv 1601

Total flavonoid content was evaluated using the colorimetric method described by Chang et al. (2002) . Briefly, one mL of extract (10000 ppm in methanol) was added with 3 mL of methanol, 0.2 mL of AlCl 3 , and 0.2 mL of sodium acetate 1 M and distilled water up to 10 mL. The solution was mixed and then incubated for 30 minutes. The absorbance was measured at 415 nm against methanol blank with a Spectrophotometer UV-Vis Seri UV-1601 (Shimadzu, Kyoto, Japan). Quercetin was used as the standard with concentration ranging from 5-19 ppm for the construction of a calibration curve, and the concentrations are expressed as quercetin equivalents (mg QE.g -1 extract). The test was performed in triplicate, and the results were expressed as mean ± SD.

One mL blood serum was put into the test tube, 0.5 mL TCA 20% was added to it, then centrifuged at 3000 rpm for 10 minutes, and the supernatant was collected. One mL of supernatant was added with 1 mL of TBA 0.67% into a tube, then placed in a water bath at a temperature of 95-100ºC for 10 minutes, then cooled with running water. Tetraethoxypropane (TEP) was used as a standard with a concentration of 0.6-2.6 nmol.mL -1 . The absorbance was measured at 532 nm using a Spectrophotometer UV-Vis Seri UV-1601 (Shimadzu, Kyoto, Japan).