Lenti x 293t cell line hek293t

SH-SY5Y cells were obtained from American Type Culture Collection (ATCC CRL-2266; Manassas, VA, USA) and cultured in Dulbecco's modified Eagle medium (D-MEM)/Ham's F-12 with L-Glutamine and phenol red (Cat# 048-29785; FUJIFILM Wako, Osaka, Japan) supplemented with 10% fetal bovine serum (Cat# FB-1365/500; Biosera, Nuaille, France) in an incubator with 5% CO2 at 37 °C. HEK293 cells were provided by RIKEN BRC, Tsukuba, Japan (Cat# RCB1637) through the National BioResource Project of MEXT, Japan, and cultured in D-MEM (high glucose) with L-Glutamine, phenol red, and sodium pyruvate (Cat# 043-30085; FUJIFILM Wako, Osaka, Japan) supplemented with 10% fetal bovine serum in an incubator with 5% CO2 at 37 °C. The Lenti-X™ 293T cell line (HEK293T) was obtained from Clontech, Mountain View, CA, USA (Cat# 632180) and cultured in the same manner as described above for HEK293 cells.

SH-SY5Y cells were obtained from American Type Culture Collection (ATCC CRL-2266; Manassas, VA, USA) and cultured in Dulbecco's modified Eagle medium (D-MEM)/Ham's F-12 with L-Glutamine and phenol red (Cat# 048-29785; FUJIFILM Wako, Osaka, Japan) supplemented with 10% fetal bovine serum (Cat# FB-1365/500; Biosera, Nuaille, France) in an incubator with 5% CO2 at 37 °C. HEK293 cells were provided by RIKEN BRC, Tsukuba, Japan (Cat# RCB1637) through the National BioResource Project of MEXT, Japan, and cultured in D-MEM (high glucose) with L-Glutamine, phenol red, and sodium pyruvate (Cat# 043-30085; FUJIFILM Wako, Osaka, Japan) supplemented with 10% fetal bovine serum in an incubator with 5% CO2 at 37 °C. The Lenti-X™ 293T cell line (HEK293T) was obtained from Clontech, Mountain View, CA, USA (Cat# 632180) and cultured in the same manner as described above for HEK293 cells.