Log In Sign up
The largest database of trusted experimental protocols

Hrp conjugated secondary f ab 2 ab

Manufactured by Zymo Research
Visit Supplier
Sourced in United States

HRP-conjugated secondary F(ab′)2 Ab is a laboratory reagent used in immunoassays. It is a secondary antibody fragment conjugated with horseradish peroxidase enzyme. The core function of this product is to provide a detection system for target antigens in various immunological techniques.

Automatically generated - may contain errors

Lab products found in correlation

P stat3, by Cell Signaling Technology (2 mentions) Il 12p35, by Santa Cruz Biotechnology (2 mentions) β actin, by Santa Cruz Biotechnology (2 mentions) Ecl plus system, by Cytiva (2 mentions) Cyclin e, by Santa Cruz Biotechnology (2 mentions) P27kip1, by Santa Cruz Biotechnology (2 mentions) Cyclin d1, by Santa Cruz Biotechnology (2 mentions) P stat4, by Cell Signaling Technology (1 mentions) P stat1, by Cell Signaling Technology (1 mentions) P stat1, by Santa Cruz Biotechnology (1 mentions) Stat4, by Santa Cruz Biotechnology (1 mentions) P stat3, by Santa Cruz Biotechnology (1 mentions)

2 protocols using hrp conjugated secondary f ab 2 ab

1

Western Blot Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Preparation of whole cell lysates and performance of Western blot analysis were as described previously (20 (link)). Cell extracts (20–40 μg/lane) were fractionated on 4–12% gradient SDS-PAGE, and antibodies used were: pSTAT1, pSTAT3, and pSTAT4 (Cell Signaling Technology); IL-12p35, Ebi3, Cyclin E, cyclin D1, p27Kip1, and β-actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Pre-immune serum was used in parallel as controls, and signals were detected with HRP-conjugated secondary F(ab′)2 Ab (Zymed Laboratories) using the ECL-PLUS system (Amersham, Arlington Heights, IL, USA).
Choi J.K., Dambuza I.M., He C., Yu C.R., Uche A.N., Mattapallil M.J., Caspi R.R, & Egwuagu C.E. (2017). IL-12p35 Inhibits Neuroinflammation and Ameliorates Autoimmune Encephalomyelitis. Frontiers in Immunology, 8, 1258.
+ Open protocol
+ Expand
2

Western Blot Analysis of STAT Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Preparation of whole cell lysates and performance of western blot analysis were as described in ref. 51 (link). Cell extracts (20–40 µg/lane) were fractionated on 4–12% gradient SDS-PAGE, and antibodies used were: pSTAT3 (Cell Signaling Technology, Danvers Massachusettes); IL-12p35, Ebi3, Cyclin E, cyclin -D1, P27Kip1, pSTAT1, pSTAT3, pSTAT4, STAT4, and β-actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA) (Supplementary Table 2). Pre-immune serum was used in parallel as controls and signals were detected with HRP-conjugated secondary F(ab′)2 Ab (Zymed Laboratories) using the ECL-PLUS system (Amersham, Arlington Heights, IL, USA).
Dambuza I.M., He C., Choi J.K., Yu C.R., Wang R., Mattapallil M.J., Wingfield P.T., Caspi R.R, & Egwuagu C.E. (2017). IL-12p35 induces expansion of IL-10 and IL-35-expressing regulatory B cells and ameliorates autoimmune disease. Nature Communications, 8, 719.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!