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Hhpa036792

Manufactured by Atlas Antibodies
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Sourced in Sweden

The HHPA036792 is a laboratory instrument designed for protein detection and quantification. It utilizes an electrochemical detection method to analyze samples. The core function of this product is to provide accurate and reliable protein measurements in a laboratory setting.

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Lab products found in correlation

Ab169276, by Abcam (2 mentions) Mousemonoclonal 6 11 b1, by Merck Group (2 mentions) Apotome axiovert 200, by Zeiss (2 mentions) Hpa 036791, by Atlas Antibodies (2 mentions) Ab27043, by Abcam (1 mentions)

2 protocols using hhpa036792

1

Immunofluorescence Analysis of Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence and cell culture analyses were performed as
previously described.(Schmidts et al.,
2015) Briefly, IMCD3, hTERT-RPE, SH-SY5Y cells and patient as well as
control fibroblasts were cultured in DMEM-F12 medium with 5% FBS under standard
conditions. For ciliation experiments, cells were serum starved for 24 hours.
Primary Antibodies used: Golgi marker GM130 (mouse, ab169276, Abcam, USA), Golgi
marker 58K (ab27043 mouse, Abcam, USA), early endosome marker EEA1 (1G11
ab70521, mouse monoclonal, Abcam), PPP1R21 antibody (rabbit, HHPA036792 (ab1)
and HPA 036791 (ab2), Atlas antibodies, Sweden), acetylated tubulin (mouse
monoclonal 6-11-B1, Sigma, USA). In brief, cells were grown on coverslips,
washed with PBS and fixed 5 minutes in 5% PFA. After 3 washes, cells were
permeabilized using 0.1% Triton for 3 minutes, washed 3 times and the incubated
in 5% BSA for 1 hour at RT to bock unspecific reactions. Incubation in primary
antibodies was then performed for 1 hour (1:200 in 5% BSA), after 5 washes,
cells were incubated in corresponding fluorescence tagged secondary 1:1000
antibody dilutions for 30 minutes, washed 5 times in PBS and then mounted in
Vectashield with DAPI. mages were taken with a Zeiss Apotome Axiovert 200 and
processed with AxioVision 4.8 and Fiji software.
Rehman A.U., Najafi M., Kambouris M., Al‐Gazali L., Makrythanasis P., Rad A., Maroofian R., Rajab A., Stark Z., Hunter J.V., Bakey Z., Tokita M.J., He W., Vetrini F., Petersen A., Santoni F.A., Hamamy H., Wu K., Al‐Jasmi F., Helmstädter M., Arnold S.J., Xia F., Richmond C., Liu P., Karimiani E.G., Karami Madani G., Lunke S., El‐Shanti H., Eng C.M., Antonarakis S.E., Hertecant J., Walkiewicz M., Yang Y, & Schmidts M. (2018). Biallelic loss of function variants in PPP1R21 cause a neurodevelopmental syndrome with impaired endocytic function. Human Mutation, 40(3), 267-280.
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2

Immunofluorescence and Cell Culture Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence and cell culture analyses were performed as previously described (Schmidts et al., 2015). Briefly, IMCD3, hTERT‐RPE, SH‐SY5Y cells and patient as well as control fibroblasts were cultured in DMEM‐F12 medium with 5% FBS under standard conditions. For ciliation experiments, cells were serum starved for 24 hr. Primary antibodies used: Golgi marker GM130 (mouse, ab169276, Abcam, USA), Golgi marker 58K (ab27043 mouse, Abcam, USA), early endosome marker EEA1 (1G11 ab70521, mouse monoclonal, Abcam), PPP1R21 antibody (rabbit, HHPA036792 (ab1) and HPA 036791 (ab2), Atlas antibodies, Sweden), acetylated tubulin (mouse monoclonal 6‐11‐B1, Sigma, USA). In brief, cells were grown on coverslips, washed with PBS and fixed 5 min in 5% PFA. After three washes, cells were permeabilized using 0.1% Triton for 3 min, washed three times and incubated in 5% BSA for 1 hr at RT to bock unspecific reactions. Incubation in primary antibodies was then performed for 1 hr (1:200 in 5% BSA), after five washes, cells were incubated in corresponding fluorescence tagged secondary 1:1,000 antibody dilutions for 30 min, washed five times in PBS and then mounted in Vectashield with DAPI. Images were taken with a Zeiss Apotome Axiovert 200 and processed with AxioVision 4.8 and Fiji software.
Rehman A.U., Najafi M., Kambouris M., Al‐Gazali L., Makrythanasis P., Rad A., Maroofian R., Rajab A., Stark Z., Hunter J.V., Bakey Z., Tokita M.J., He W., Vetrini F., Petersen A., Santoni F.A., Hamamy H., Wu K., Al‐Jasmi F., Helmstädter M., Arnold S.J., Xia F., Richmond C., Liu P., Karimiani E.G., Karami Madani G., Lunke S., El‐Shanti H., Eng C.M., Antonarakis S.E., Hertecant J., Walkiewicz M., Yang Y, & Schmidts M. (2018). Biallelic loss of function variants in PPP1R21 cause a neurodevelopmental syndrome with impaired endocytic function. Human Mutation, 40(3), 267-280.
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