MHCC97-H, HLE, Huh-7, and PLC/PRF/5 cell lines were purchased form Guangzhou Jennio Biotech Co., Ltd., Guangzhou, China. All cells were validated by STR DNA fingerprinting and tested for mycoplasma. MHCC97-H and Huh-7 cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (pen/strep). HLE cells were cultured in minimum essential medium (MEM, Sigma) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. PLC/PRF/5 cells were cultured in Roswell Park MEMorial Institute (RPMI) 1640 medium (Sigma) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. All cells were maintained at 37 °C in a humidified 5% CO2 atmosphere.
Huh 7
Manufactured by Jennio Biotech
Sourced in China
The Huh-7 is a laboratory cell line derived from a hepatocellular carcinoma. It is a widely used in vitro model for studying various aspects of liver biology and disease.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Mhcc97 h, by Jennio Biotech (2 mentions)
Plc prf 5, by Jennio Biotech (2 mentions)
Hepg2, by Jennio Biotech (2 mentions)
1640 medium, by Merck Group (1 mentions)
Minimum essential medium (mem), by Merck Group (1 mentions)
Mhcc97h, by Merck Group (1 mentions)
Plc prf 5, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Penicillin strepomycin, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
4 protocols using huh 7
1
Cell Line Validation and Cultivation Protocol
2
Quantitative Analysis of Gene Expression in Liver Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human hepatic cell line (LO2) and HCC cell lines (Huh-7 and HepG2) were obtained from Guangzhou Jennio Biotech Co., Ltd. (Shanghai, China). All cells were maintained in dulbecco's modified eagle medium (Invitro-gen, Carlsbad, CA, USA) and 10% fetal bovine serum (Invitrogen) with 1% penicillin-strepomycin (Invitrogen) at 37%℃ and 5% CO2. Based on the manufacturer's protocol, total tissue RNA was extracted using TRIzol regent (Tiangene, China). Subsequently, the RNA was transcribed into cDNA. Then, with the cDNA as the template, qRT-PCR was carried out. Relative mRNA expression was calculated using the 2−ΔΔCt method.
3
Establishment and Authentication of Liver Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Huh-7, PLC/PRF/5, MHCC97H, Hep3B, and Immortalized human liver cell line (L02) cells were obtained from Jennio Biotech Co. Ltd. (Guangzhou, China). The cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS; Gibco) at 37 °C and 5% CO2. The cells were authenticated via short tandem repeat analysis every 6 months. Human HCC tissues and paired non-tumor adjacent tissues were obtained from the First Affiliated Hospital of Shenzhen University (Shenzhen, China). The ethics application was approved by the Ethics Committee (application number KS20190903002) of the First Affiliated Hospital of Shenzhen University. Written informed consent was obtained from all the patients.
4
Cell Culture of Liver Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!