The lymphoblastic cell line (YH cell line) was established from an Asian genome donor [35] (link). We purchased the HeLa S3 cell line from the American Type Culture Collection (Catalog No. CCL-2.2, ATCC, Manassas, VA). The tumor sample used for on-chip reaction determination was a resected sample of a 45-year-old male patient (HCC01) with a primary HCC tumor. Paired primary and relapsed HCC tumor samples were obtained from a 63-year-old male patient (HCC02). Peripheral white blood cells, paired tumor samples, and ANT were also obtained for bulk WES or WGS.
Hela s3 cell line
Manufactured by American Type Culture Collection
Sourced in United States
The HeLa S3 cell line is a clonal derivative of the original HeLa cell line, which was derived from cervical cancer cells obtained from Henrietta Lacks. The HeLa S3 cell line is a continuous, adherent cell line that can be used for a variety of cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Rucaparib, by Selleck Chemicals (1 mentions)
Doxorubicin, by Santa Cruz Biotechnology (1 mentions)
Hyclone, by GE Healthcare (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Ccl 22, by American Type Culture Collection (1 mentions)
4 protocols using hela s3 cell line
1
Asian Genome Donor Cell Line
2
Doxorubicin and Rucaparib Cytotoxicity Assay
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Human HeLa S3 cell line (HeLa) was obtained from American Type Culture Collection. The cells were cultured in DMEM (HyClone; GE Healthcare Bio-Sciences Corp., Piscataway, NJ, USA) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA), 100 U/mL penicillin and 100 ng/mL streptomycin at 37°C in a humidified atmosphere of 95% air/5% CO2. Doxorubicin (sc-280681) was purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Rucaparib was purchased from Selleck (AG-014699). For Doxorubicin treatment, cells were cultured in fresh culture medium with Doxorubicin (0, 100, 200, 300, 500 and 750 μM) for 24 hours. For PARP1 inhibitor treatment, cells were cultured in fresh culture medium with Rucaparib (1 nM) for 7 days.
3
UVC-Induced DNA Damage and Repair in HeLa-S3 Cells
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The HeLa-S3 cell line (purchased from American Type Culture Collection) was cultured in standard Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) and 1% penicillin–streptomycin (Gibco). Cells were incubated at 37 °C in a 5% CO2 humidified chamber.
Cells at about 80% confluence were irradiated using a UVC lamp (GE) connected to a digital timer for 10 s (2 J/m2/sec) after removing the media. After irradiation, culture medium was added back and cells were incubated at 37 °C in a 5% CO2 humidified chamber for the indicated times. After repair, cells were put on ice and washed with ice-cold PBS, then harvested in cold PBS, and collected by centrifugation at 1000g at 4 °C for 5 min. For TPL (MedChemExpress) treatment, TPL was added to the medium 10 min prior to UV irradiation and maintained after UV irradiation at a final concentration of 1 μM.
Cells at about 80% confluence were irradiated using a UVC lamp (GE) connected to a digital timer for 10 s (2 J/m2/sec) after removing the media. After irradiation, culture medium was added back and cells were incubated at 37 °C in a 5% CO2 humidified chamber for the indicated times. After repair, cells were put on ice and washed with ice-cold PBS, then harvested in cold PBS, and collected by centrifugation at 1000g at 4 °C for 5 min. For TPL (MedChemExpress) treatment, TPL was added to the medium 10 min prior to UV irradiation and maintained after UV irradiation at a final concentration of 1 μM.
4
Cell Lines and Tumor Samples
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The lymphoblastic cell line (YH cell line) was established from an Asian genome donor [35] . We purchased the HeLa S3 cell line from the American Type Culture Collection (CCL-2.2, ATCC, Manassas, VA, USA). The tumour sample used for on-chip reaction determination was a resected sample of a 45-year-old male patient (HCC01) with a primary HCC tumour. Paired primary and relapsed HCC tumour samples were obtained from a 63-year-old male patient (HCC02). Peripheral white blood cells and paired tumour sample and adjacent normal liver tissue were also obtained for bulk whole-exome sequencing or whole-genome sequencing.
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