Human pancreatic adenocarcinoma cell lines

If not otherwise specified, all human pancreatic adenocarcinoma cell lines, HPNE cells, and HEK293T cells were purchased from the American Type Culture Collection. PDA cell lines MDA28 and MDA48 were gifts from Dr. Paul J. Chiao (The University of Texas MD Anderson Cancer Center). Generation of the FG human pancreatic adenocarcinoma cell line was described previously (1) . All cell lines were maintained in 5% CO2 at 37°C as an adherent monolayer in Dulbecco modified Eagle medium supplemented with 10% fetal bovine serum, sodium pyruvate, nonessential amino acids, L-glutamine, penicillin/streptomycin, and a vitamin solution (Flow Laboratories). The American Type Culture Collection performs characterization and authentication of the cell lines it provides using short tandem repeat profiling, and the cell lines they provided were passaged in our laboratory for fewer than 6 months after reception. S3. PRMT1 specific inhibitors DB75 and TC-E5003 were purchased from Tocris Bioscience. Etoposide and H2O2 were purchased from Sigma-Aldrich Corporate. Gemcitabine hydrochloride was purchased from United States Pharmacopeia (USP).

If not otherwise speci ed, all human pancreatic adenocarcinoma cell lines and mouse 266-6 acinar cells were purchased from the American Type Culture Collection (ATCC). Mouse PDA cell lines PKC-mL3 and PKC-mL1 were obtained from David Tavion's laboratory at Cold Spring Harbor Laboratory. PKC-118 is a cell line derived from the PKC mouse model. Human pancreatic stellate cell (HPSC) line was a gift from Rosa F. Hwang at The University of Texas MD Anderson Cancer Center. All of these cell lines were maintained in 5% CO 2 at 37°C as an adherent monolayer in Dulbecco modi ed Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), sodium pyruvate, nonessential amino acids, Lglutamine, penicillin/streptomycin, and a vitamin solution (Flow Laboratories). The immortalized normal human pancreatic ductal epithelial (HPDE) cell line (provided by Dr. Tsao, Ontario Cancer Institute, Toronto, ON, Canada) was maintained in keratinocyte serum-free medium supplemented with epidermal growth factor and bovine pituitary extract (Invitrogen). The ATCC performs characterization and authentication of the cell lines it provides using short tandem repeat pro ling, and the cell lines they provided were passaged in our laboratory for fewer than 6 months after reception.