In order to investigate the functional groups in the EPSs samples of B. cereus and mutants Attenuated Total Reflection Fourier Transform Infrared spectroscopy (ATR-FTIR) was used. Spectra of solid samples were recorded from 4000 cm−1 to 500 cm−1 on a Golden Gate Single Reflection Diamond ATR System (Graseby Specac) fitted into a Bruker Vertex 70 FT-IR spectrometer. Standard spectral resolution of 4 cm−1 was used and 64 scans were selected for both background and sample measurements. Spectral contributions from residual water vapor were reduced using the atmospheric compensation filter built in the Bruker OPUS software.
Golden gate single reflection diamond atr system
Manufactured by Specac
Sourced in United States
The Golden Gate Single Reflection Diamond ATR System is a laboratory instrument designed for attenuated total reflection (ATR) infrared spectroscopy. It utilizes a diamond crystal as the ATR element, providing a robust and chemically resistant sampling interface for a wide range of solid, liquid, and paste-like samples.
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3 protocols using golden gate single reflection diamond atr system
1
ATR-FTIR Spectroscopy of Bacillus cereus EPS
2
ATR-FTIR Analysis of Colloidal Dispersions
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Attenuated total reflectance Fourier Transform infrared spectroscopy (ATR-FTIR) data for the colloidal dispersions (liquid phase) and the powders (solid-phase) were measured in the range of 500 to 4000 cm À1 using a Thermo Fisher Nicolet iS5 FTIR spectrometer equipped with a Specac Golden Gate Single Reflection Diamond ATR System, KBr optics, a diamond ATR crystal, and ZnSe lenses. Each spectrum was the average of 64 scans with a spectral resolution of 2 cm À1 . Data for each colloidal dispersion (liquid phase) were collected in situ by placing a drop of the dispersion on the ATR diamond, and using a cap to block light passing though the samples during measurements.
3
FTIR Analysis of Cell Wall Material
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The FTIR spectra were obtained from total CWM powder using a PerkinElmer Spectrum GX (Waltham, MA, USA) spectrometer equipped with a Golden Gate single reflection diamond ATR system (Specac Ltd., Orpington, Kent, UK). For each sample, 64 scans were accumulated in the range of 400–4000 cm−1. Spectra were ATR and baseline corrected using the Spectrum 10.4.3 software (PerkinElmer). They were subsequently area-normalized between 1800 and 850 cm−1.
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