Cephotaxime

Manufactured by HiMedia

Sourced in India

Cephotaxime is a third-generation cephalosporin antibiotic. It is a broad-spectrum antibiotic effective against a wide range of Gram-positive and Gram-negative bacteria.

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2 protocols using cephotaxime

Enzymatic Assay for Cysteine Sulfoxide Synthesis

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Reduced
form of nicotinamide adenine dinucleotide
(NADH), lactate dehydrogenase (LDH) from rabbit muscle, and (±)-L-alliin
were purchased from Sigma-Aldrich; pyridoxal 5′-phosphate (PLP)
and d,l-dithiothreitol (DTT) were from Serva; kanamycin
is a domestic product (OAO Biokhimik); DEAE-sepharose was from Amersham.
2-Nitro-5-thiobenzoate (NTB) was prepared according to ref (31 (link)). S-Methyl-l-cysteine sulfoxide (methiin) was synthesized according to
Morozova et al.^{22 (link)} PEG–poly(α,β-aspartic
acid)₇₀ (PEG–P(Asp)₇₀) and poly-(l-lysine)₇₀ (PLL₇₀) were synthesized according
to Koide et al.^{32 (link)} Luria–Bertani
broth (LB), Mueller–Hinton broth, Mueller–Hinton agar,
and antibiotic-impregnated discs: amikacin, amoxycillin/clavulanic
acid, ampicillin, azithromycin, aztreonam, cefepime, ceftazidime,
ceftriaxone, cephotaxime, cephoxitin, chloramphenicol, cipr ofloxacin,
colistin, doxycycline, erythromycin, gentamicin, imipenem, lev ofloxacin,
lincomycin, norfloxacin, ofloxacin, oxacillin, rifampicin, spiramycin,
tobramycin, and vancomycin were from HiMedia Laboratories Pvt. Limited
(India).

Morozova E., Kulikova V., Koval V., Anufrieva N., Chernukha M., Avetisyan L., Lebedeva L., Medvedeva O., Burmistrov E., Shaginyan I., Revtovich S, & Demidkina T. (2020). Encapsulated Methionine γ-Lyase: Application in Enzyme Prodrug Therapy of Pseudomonas aeruginosa Infection. ACS Omega, 5(14), 7782-7786.

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Antibiotic Susceptibility of Common Bacterial Strains

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Six bacterial strains, Bacillus pumilus MTCC 1607, Streptococcus aureus MTCC 902, Escherichia coli MTCC 1304, Klebsiella pneumoniae MTCC 3384, Pseudomonas aeruginosa MTCC 741 and Salmonella typhi MTCC 537, were grown at 160 rpm in nutrient broth (NB) at 32 °C. Seventeen antibiotics discs named Amikacin (Ak) 30 µg, Ampicillin (A) 10 µg, Ampicillin/Sulbactam (Ams) 10/10 µg, Amoxyclav (Ac) 30 µg, Ceftazidime (Ca) 30 µg, Cephotaxime (Ce) 30 µg, Ciprofloxacin (Cf) 5 µg, Clindamycin (Cd) 2 µg, Co- Trimoxazole (Co) 25 µg, Erythromycin (E) 15 µg, Gentamycin (G) 10 µg, Nalidixic acid (Na) 30 µg, Netillin (Nt) 30 µg, Nitrofurantoin (Nf) 300 µg, Penicillin G (P) 10 units, Tobramycin (Tb) 10 µg and Vancomycin (Va) 3 µg, were procured from HiMedia Laboratories Pvt. Ltd., India.

Sharma N., Singh V., Pandey A.K., Mishra B.N., Kulsoom M., Dasgupta N., Khan S., El-Enshasy H.A, & Haque S. (2019). Preparation and Evaluation of the ZnO NP–Ampicillin/Sulbactam Nanoantibiotic: Optimization of Formulation Variables Using RSM Coupled GA Method and Antibacterial Activities. Biomolecules, 9(12), 764.

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