Gx 271 preparative scale reverse phase hplc system

All compounds that are designated as being purified by preparative HPLC were purified according to the same method, as described below. Purification was carried out using a Gilson GX-271 preparative scale reverse phase HPLC system with Gilson model 159 UV-VIS detector and Phenonemex Kinetex 5 μm, C18, 100 Å, 50 mm × 30 mm column. Compounds were eluted using a gradient elution of A:B (90:10 to 0:100) over 5 min at a flow rate of 50.0 mL/min, where solvent A was H₂O (with 0.1% formic acid) and solvent B was CH₃CN (with 0.1% formic acid). The product fractions were combined and dried using a Genevac EZ-2 Centrifugal Evaporator.

All compounds that are designated as being purified by preparative HPLC were purified according to the same method, as described here. Purification was carried out using a Gilson GX-271 preparative scale reverse phase HPLC system with Gilson model 159 UV-VIS detector and Phenonemex Kinetex 5 μm, C18, 100 Å, 50 mm × 30 mm column. Compounds were eluted using a gradient mobile phase of A:B (90:10 to 0:100) over 5 min at a flow rate of 50.0 mL/min, where solvent A was H₂O (with 0.1% formic acid) and solvent B was CH₃CN (with 0.1% formic acid). The product fractions were combined and dried using a Genevac EZ-2 Centrifugal Evaporator.