Instruments used were an evaporator Rotavapor RV 10 Digital V connected to heating bath HB digital and RV 10.1 set of glassware vertical (IKA Id. no. 0010004799), chemical glassware (Iwaki Pyrex), autoclave (all American type 75X), Biological Safety Cabinet (BSC) type-2, microplate reader (Infinite M200 Pro, Tecan), microplate reader filter 450–490 nm, inverted microscope (Zeiss), sterilized 96-well microplates, multichannel pipettes (8 or 12 channels: 10–100 μL), CO2 incubator (Heracell VIOS 250i, Thermo Scientific), and hemocytometer (cell counter).
Heracell vios 250i
Manufactured by Thermo Fisher Scientific
Sourced in China, United States
The Heracell VIOS 250i is a laboratory incubator designed for cell culture applications. It features an internal volume of 250 liters and provides temperature and CO2 control for maintaining optimal environmental conditions for cell growth.
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Lab products found in correlation
4 protocols using heracell vios 250i
1
Cytotoxicity Assay in 96-well Microplates
2
HSF Cell Culture Conditions
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The HSF used for the experiments were between 5 and 20 generations. The HSF were placed in a CO2 incubator (Heracell™ VIOS 250i, Thermo Fisher Scientific (China) Co., Ltd.) and grown in a complete DMEM medium (containing 1% penicillin–streptomycin and 10% FBS). The medium was changed every 48 h and dissociated using 0.25% trypsin and 0.02% EDTA. All tests were carried out for 12 h after the cells were seeded at 1 × 104 cells/well in 96-well microplates (or 5 × 105 cells/well in 6-well microplates).
3
Culturing Murine Brain Endothelial Cells
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Murine microvascular brain endothelial cells (cerebEND) [23 (link),24 (link),25 (link),26 (link)] were grown in Dulbecco’s Modified Eagle’s Medium (DMEM) supplied with 1% penicillin/streptomycin and 10% fetal calf serum (FCS) on gelatin-coated 6- or 96-well plates (Thermo Fisher Scientific, Waltham, MA, USA). They were cultured in a 37 °C incubator (Heracell VIOS 250i, Thermo Fisher Scientific, USA) until 95% confluent as observed under the microscope.
4
Zika Virus Antiviral Assay Protocol
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Vero cells (CCL-81) were used in all phases of the in vitro testing. The cultivation was performed with products from the company, Thermo Fisher Scientific. DMEM supplemented with 10% foetal bovine serum (FBS) and 1% penicillin/streptomycin and incubated at 37 °C and 5% CO2 atmosphere on incubator Heracell VIOS 250i (Thermo Fisher Scientific).
A strain of ZIKV/H.sapiens/Brazil/PE243/2015 (abbreviated to ZIKV PE243; GenBank accession number KX197192.1) was used in antiviral tests. The viral stock was made in Vero cells and grown at 37 °C and 5% CO2 atmosphere. The endpoint dilution assay was the method used to obtain the TCID50/mL titer of this stock.
A strain of ZIKV/H.sapiens/Brazil/PE243/2015 (abbreviated to ZIKV PE243; GenBank accession number KX197192.1) was used in antiviral tests. The viral stock was made in Vero cells and grown at 37 °C and 5% CO2 atmosphere. The endpoint dilution assay was the method used to obtain the TCID50/mL titer of this stock.
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