Cells were incubated with PE-conjugated anti-PDGFRA (CD140a) antibody (Biolegend, clone 16A1) for 30 minutes at room temperature at the dilution specified in the manufacturer’s protocol. Data was analyzed and visualized with FlowJo software. Single live cells were selected for analysis via side and forward scatter, and viable cells were selected by lack of an unstained channel (APC) autofluorescence.
Pe conjugated anti pdgfra cd140a antibody
Manufactured by BioLegend
The PE-conjugated anti-PDGFRA (CD140a) antibody is a laboratory reagent used to detect and analyze the expression of the PDGFRA (platelet-derived growth factor receptor alpha) protein, also known as CD140a, on the surface of cells. This antibody is conjugated with the fluorescent dye R-Phycoerythrin (PE), which allows for the visualization and quantification of PDGFRA-expressing cells using flow cytometry or other fluorescence-based detection methods.
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2 protocols using pe conjugated anti pdgfra cd140a antibody
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PDGFRA Expression Analysis by Flow
2
PDGFRA Expression Analysis by Flow
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Cells were incubated with PE-conjugated anti-PDGFRA (CD140a) antibody (Biolegend, clone 16A1) for 30 minutes at room temperature at the dilution specified in the manufacturer’s protocol. Data was analyzed and visualized with FlowJo software. Single live cells were selected for analysis via side and forward scatter, and viable cells were selected by lack of an unstained channel (APC) autofluorescence.
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