Mouse anti 53bp1

Manufactured by Merck Group

Mouse anti-53BP1 is a laboratory tool used to detect the 53BP1 protein, which plays a critical role in DNA damage response and repair pathways. This product is a purified monoclonal antibody that specifically binds to the 53BP1 protein, allowing researchers to study its expression and localization in cells.

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Lab products found in correlation

Goat anti rabbit alexa 546, by Thermo Fisher Scientific (1 mentions) Goat anti mouse alexa 488, by Thermo Fisher Scientific (1 mentions) Mouse anti flag m2, by Merck Group (1 mentions) Anti γh2ax, by Merck Group (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) Ab2175, by Santa Cruz Biotechnology (1 mentions) Ab10805, by Abcam (1 mentions) Mouse anti pcna, by Santa Cruz Biotechnology (1 mentions) Sirnas, by Horizon Discovery (1 mentions) Mab3802, by Merck Group (1 mentions) Mab374, by Abcam (1 mentions) Mouse anti p53, by Santa Cruz Biotechnology (1 mentions) Mouse anti histone h3, by Abcam (1 mentions) Rabbit anti cyclin a, by Santa Cruz Biotechnology (1 mentions) Smartpool, by Horizon Discovery (1 mentions)

Close spelling variants of this product

Anti-53BP1 (8 citations)

2 protocols using mouse anti 53bp1

Immunofluorescence Analysis of DNA Damage Response

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iMEFs were grown on coverslips overnight. Cells were irradiated (10 Gy IR) and allowed to recover for 90 min or 6 h. Upon fixation with 4% paraformaldehyde (Sigma-Aldrich) and permeabilization with 0.5% Triton X-100, cells were stained with anti-γH2AX (Millipore), rabbit anti-Rif1 serum (Di Virgilio et al., 2013 (link)), mouse anti-53BP1 (Millipore), mouse anti-Flag M2 (Sigma-Aldrich) or rabbit anti-ZMYND8 (Sigma-Aldrich) antibodies as primary antibodies, and with goat anti-rabbit Alexa546 and goat anti-mouse Alexa488 (Invitrogen) as secondary antibodies. Images were acquired using inverted LSM700 laser scanning confocal microscope (Zeiss).

Delgado-Benito V., Rosen D.B., Wang Q., Gazumyan A., Pai J.A., Oliveira T.Y., Sundaravinayagam D., Zhang W., Andreani M., Keller L., Kieffer-Kwon K.R., Pękowska A., Jung S., Driesner M., Subbotin R.I., Casellas R., Chait B.T., Nussenzweig M.C, & Di Virgilio M. (2018). The Chromatin Reader ZMYND8 Regulates Igh Enhancers to Promote Immunoglobulin Class Switch Recombination. Molecular Cell, 72(4), 636-649.e8.

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Investigating DNA Damage Response Mechanisms

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siRNAs were obtained from Dharmacon. HMCES: D-020333–01 5’-GCGAACAUCCUGUCACUUA, J-020333–19 5’-CGUAAUGGAGAAACGGUCA, and J020333–20 5’-ACCAACUGUCGUAGUGAUA. Smartpools were purchased from Dharmacon for REV3L, Rad18, USP1, SHPRH, BRCA1, and BRCA2. The following antibodies were used: rabbit anti-HMCES (Sigma, HPA044968); mouse anti-GAPDH (Millipore, MAB374); mouse anti-RPA32 (Abcam, ab2175); rabbit anti-Cyclin A (Santa Cruz, sc-751); mouse anti-PCNA (Santa Cruz, sc-56); mouse anti-Histone H3 (Abcam, ab10799); mouse anti-FLAG M2 (Sigma, F3165); 5-methylcytosine (Abcam, ab10805); 5-hydroxymethylcytosine (Abcam, ab106918); ssDNA antibody (Millipore, MAB3034); mouse anti-p21 (Cell Signaling, 2946); mouse anti-p53 (Santa Cruz, sc-126); mouse anti-53BP1 (Millipore, MAB3802).

Mohni K.N., Wessel S.R., Zhao R., Wojciechowski A.C., Luzwick J.W., Layden H., Eichman B.F., Thompson P.S., Mehta K.P, & Cortez D. (2018). HMCES maintains genome integrity by shielding abasic sites in single strand DNA. Cell, 176(1-2), 144-153.e13.

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