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Goat anti rabbit igg rhodamine

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Goat anti-rabbit IgG Rhodamine is a secondary antibody used in immunodetection applications. It is produced by immunizing goats with rabbit IgG and conjugating the resulting antibodies with the rhodamine fluorescent dye.

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Lab products found in correlation

Fluorescein goat anti mouse igg, by Thermo Fisher Scientific (3 mentions) Mouse anti flag monoclonal ab, by Merck Group (2 mentions) Rabbit anti human svep1 primary antibody, by Abcam (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Mouse monoclonal anti ha antibody, by Merck Group (1 mentions) Fv1000 confocal microscope, by Olympus (1 mentions) Rabbit polyclonal anti myc antibody, by Santa Cruz Biotechnology (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Pik93, by Merck Group (1 mentions) Mouse anti myc, by Santa Cruz Biotechnology (1 mentions) Rabbit anti lc3b, by Cell Signaling Technology (1 mentions) Anti atg5, by Cell Signaling Technology (1 mentions) Anti rpl5, by Cell Signaling Technology (1 mentions) Anti atg7, by Cell Signaling Technology (1 mentions) Anti pink1, by Cell Signaling Technology (1 mentions) Anti ndp52, by Cell Signaling Technology (1 mentions) Anti ubiquitin, by Santa Cruz Biotechnology (1 mentions) Lsm 700 confocal microscope, by Zeiss (1 mentions) Mouse anti human ki 67 antibody, by Agilent Technologies (1 mentions)

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IgG Rabbit (4 citations)

5 protocols using goat anti rabbit igg rhodamine

1

Immunofluorescence Imaging of Viral Protein Localization

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HeLa cells grown on coverslips in 24-well-plates were transfected with 0.5 µg pCAGGS-P, 0.125 µg pCAGGS-N and increasing amounts of pCAGGS-M or 0.5 µg M mutants by Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA). 24 h pt, cells were washed with cold PBS and fixed with 4% paraformaldehyde for 20 min. Then, the cells were permeabilised with 0.2% Triton X-100 for 20 min at room temperature. Blocking the permeated cells by 3% bovine serum albumin (BSA) in PBS for 0.5 h at room temperature and incubating the cells with mouse monoclonal anti-HA antibody (Sigma, St. Louis, MI, USA; 1:2000) and rabbit polyclonal anti-Myc antibody (Santa Cruz, CA, USA; 1:200) in 1% BSA at room temperature for 2 h. Then, the cells were washed with 1% BSA in PBS and incubated with the goat anti-mouse IgG fluorescein (Thermo; 1:200) and goat anti-rabbit IgG rhodamine (Thermo; 1:100) secondary antibody for 2 h at room temperature. After being washed three times with 1% BSA in PBS, the coverslips were turned over and the cells were incubated with a drop of DAPI (4′,6-diamidino-2-phenylindole)-contained Fluorshield for nuclear staining. Confocal images were collected by an Olympus confocal FV1000 microscope (Olympus, Tokyo, Japan).
Zhang S., Cheng Q., Luo C., Qin Y, & Chen M. (2018). Human Parainfluenza Virus Type 3 Matrix Protein Reduces Viral RNA Synthesis of HPIV3 by Regulating Inclusion Body Formation. Viruses, 10(3), 125.
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2

Antibody Acquisition and Reagent Details

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Mouse anti-TGN46 monoclonal Ab was purchased from Sigma-Aldrich. Mouse anti-Tom20 monoclonal Ab was purchased from BD Biosciences. Rabbit anti-Calnexin polyclonal antibodies (Abs) was purchased from Sigma-Aldrich. Rabbit anti-PDI polyclonal Ab was purchased from Abcam. Rabbit anti-LMNB1 Ab was purchased from ABclonal. Rabbit anti-PI4KA polyclonal Ab was purchased from ABclonal Technology. Rabbit anti-PI4B and anti-PI4K2B polyclonal Abs were purchased from Abcam. Rabbit anti-PI42A polyclonal Ab was purchased from Cell Signaling Technology. Mouse anti-HPIV3 HN monoclonal Ab was purchased from Abcam. Mouse anti-PI4P monoclonal Ab was purchased from Echelon Biosciences. Mouse anti-Flag monoclonal Ab, rabbit anti-Flag polyclonal Ab, and mouse anti-HA monoclonal Ab were purchased from Sigma-Aldrich. Mouse anti-Myc and anti-GAPDH monoclonal Abs were purchased from Santa Cruz Biotechnology, Inc. Goat anti-rabbit IgG Rhodamine and goat anti-mouse IgG Fluorescein were purchased from Thermo. DAPI was purchased from Sigma-Aldrich. PIK93 was purchased from Sigma-Aldrich and dissolved in dimethylsulfoxide (DMSO). Thapsigargin (Tg) was a gift from Y. Liu Lab (Wuhan University). Tunicamycin (Tu) was purchased from Med Chem Express (MCE) and dissolved in DMSO.
Li Z., Guo D., Qin Y, & Chen M. (2019). PI4KB on Inclusion Bodies Formed by ER Membrane Remodeling Facilitates Replication of Human Parainfluenza Virus Type 3. Cell Reports, 29(8), 2229-2242.e4.
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3

Antibody Profiling for Cell Analysis

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Rabbit anti-LC3B, anti-Atg5, anti-Atg7, anti-PINK1, anti-NDP52, anti-PA28, anti-PSMA3, anti-L7a, anti-RPL5 and anti-p62 polyclonal antibodies (Abs) were purchased from Cell Signaling Technology. Mouse anti-COXII monoclonal Ab was purchased from Abcam. Rabbit anti-TUFM polyclonal Ab, mouse anti-Flag monoclonal Ab, rabbit anti-Flag polyclonal Abs, and mouse anti-HA monoclonal Abs were purchased from Sigma Aldrich. Rabbit anti-Myc, anti-Beclin1, anti-GFP polyclonal Abs, mouse anti-Myc, anti-ubiquitin and anti-GAPDH monoclonal Abs were purchased from Santa Cruz Biotechnology Inc. Mouse anti-TIM23 and anti-TOM20 were purchased from BD Biosciences. Anti-HPIV3 HN monoclonal Ab was purchased from Abcam. Anti-GST polyclonal Ab was purchased from Amersham Bioscience. Rabbit anti-VISA polyclonal Ab was achieved from Shu HB Lab. Goat anti-rabbit IgG Rhodamine and goat anti-mouse IgG Fluorescein were purchased from Thermo. Goat anti-mouse IgG DyLight 350 was purchased from Abbkine. BAF and CCCP were purchased from Sigma Aldrich. ExRed was purchased from Zoman Biotechnology.
Ding B., Zhang L., Li Z., Zhong Y., Tang Q., Qin Y, & Chen M. (2017). The Matrix Protein of Human Parainfluenza Virus Type 3 Induces Mitophagy that Suppresses Interferon Responses. Cell host & microbe, 21(4).
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4

Immunofluorescence Staining of SVEP1 in KCs and Fibroblasts

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KCs and fibroblasts that were harvested from punch biopsies were grown on glass coverslips and fixed with 4% paraformaldehyde. Following permeabilization with 0.5% Triton/PBS, slides were blocked in 3% BSA, 2% normal goat serum and 0.2% triton in PBS for 20 min at room temperature. Rabbit anti human SVEP1 primary antibody (Abcam, Cambridge, MA) was diluted 1:30 in blocking solution. The cells were incubated for 1h at room temperature. The cells were then incubated for 45 min at room temperature with a secondary antibody consisting of rhodamine goat anti rabbit IgG (Invitrogen, Carlsbad, CA) diluted 1:200 in PBS. Following several washing steps, coverslips were mounted in DAPI Fluoromount-G (Southern Biotechnologies, Birmingham, AL).
Samuelov L., Li Q., Bochner R., Najor N., Albrecht L., Malchin N., Goldsmith T., Grafi-Cohen M., Vodo D., Fainberg G., Meilik B., Goldberg I., Warshauer E., Rogers T., Edie S., Ishida-Yamamoto A., Burzenski L., Erez N., Murray S., Irvine A., Shultz L., Green K., Uitto J., Sprecher E, & Sarig O. (2017). SVEP1 plays a crucial role in epidermal differentiation. Experimental dermatology, 26(5), 423-430.
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5

SVEP1 Immunofluorescence Analysis of Skin Biopsies

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For SVEP1 immunofluorescence analysis of skin biopsies, 5 µm paraffin-embedded sections were baked overnight at 37°C and de-paraffinized using xylene/ethanol. Antigen retrieval was accomplished using 0.01 M citrate buffer, pH 6.0. Sections were blocked with 2% BSA in PBS for 30 min at room temperature. Rabbit anti human SVEP1 primary antibody (Abcam, Cambridge, MA) was diluted 1:30 with 2% BSA in PBS and incubated overnight at 4°C. Rhodamine goat anti rabbit IgG (Invitrogen, Carlsbad, CA) was used as a secondary antibody and was diluted 1:200 with 2% BSA in PBS and was incubated for 45 min at room temperature. Coverslips were mounted in DAPI Fluoromount-G (Southern Biotechnologies, Birmingham, AL). Negative control was obtained by omitting the primary antibody. As a positive control, we used a normal placenta tissue (5 (link)) which stained positively with the antibody (Fig. S1). Samples were examined using either a Nikon 50I microscope connected to DS-RI1 digital camera or a Zeiss LSM700 confocal microscope for fluorescence image acquisition.
Immunostaining with mouse anti human Ki-67 antibody (DAKO, Hamburg, Germany) was performed as previously described (20 (link)).
Samuelov L., Li Q., Bochner R., Najor N., Albrecht L., Malchin N., Goldsmith T., Grafi-Cohen M., Vodo D., Fainberg G., Meilik B., Goldberg I., Warshauer E., Rogers T., Edie S., Ishida-Yamamoto A., Burzenski L., Erez N., Murray S., Irvine A., Shultz L., Green K., Uitto J., Sprecher E, & Sarig O. (2017). SVEP1 plays a crucial role in epidermal differentiation. Experimental dermatology, 26(5), 423-430.
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