Isolated single cells derived from tumors were cultured in X–VIVO 15 medium (Lonza, Switzerland) supplemented with 2% human serum (Biological Industries, USA) at 37°C and 5% CO2. Cells were treated with an anti-PD-1 antibody (pembrolizumab, 20 μg/ml) for 24 hours and then subjected to phenotypic analysis by flow cytometry.
Human serum
Manufactured by Sartorius
Sourced in United States
Human serum is a biological fluid that is derived from the blood of human individuals. It contains a variety of proteins, hormones, and other biochemical components that are essential for various physiological processes. This lab equipment is commonly used in scientific research, clinical diagnostics, and medical testing applications.
Automatically generated - may contain errors
Lab products found in correlation
X vivo 15 medium, by Lonza (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions)
Biowhittaker, by Lonza (1 mentions)
Newborn calf serum, by Sartorius (1 mentions)
Rpmi 1640 with ultraglutamine, by Lonza (1 mentions)
2 protocols using human serum
1
Tumor Cell Profiling with Anti-PD-1
2
Neonatal Rat Islet Isolation and Culture
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Neonatal rat islets of Langerhans were isolated from 4-day-old Wistar rat pups (Taconic, Lille Skensved, Denmark), as previously described [20] . All mice were housed according to the Principles of Laboratory Care. The isolated islets were pre-cultured for 7-10 days in RPMI 1640 with UltraGlutamine (Lonza, Vallensbaek, Denmark) and supplemented with 10% newborn calf serum (Biological Industries, Kibbutz Beit Haemek, Israel), 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco, Life Technologies, Taastrup, Denmark) in 5% CO 2 at 37°C. For experiments, rat islets were cultured as intact and free-floating in medium supplemented with 2% human serum (BioWhittaker, Lonza) or as single cells following digestion with 0.2% trypsin (Gibco) and 10 mmol/l EDTA (Gibco) in Hanks' balanced salt solution (HBSS). Dispersed islets were cultured on coverslips coated with bovine corneal extracellular matrix (ECM; Biological Industries) in the medium described above containing 2% human serum.
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