Log In Sign up
The largest database of trusted experimental protocols

Pretroq

Manufactured by Takara Bio
Visit Supplier

The PRetroQ is a laboratory instrument manufactured by Takara Bio. It is designed to perform quantitative analysis of retroviral RNA samples. The core function of the PRetroQ is to accurately measure the concentration of retroviral RNA in a given sample.

Automatically generated - may contain errors

Lab products found in correlation

Pcdna3, by Thermo Fisher Scientific (1 mentions) Anti tau1, by Merck Group (1 mentions) Anti e cadherin, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions) Anti mlc, by Cell Signaling Technology (1 mentions) Anti na k atpase, by Abcam (1 mentions) Anti trka, by Cell Signaling Technology (1 mentions) Anti phospho mlc, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

PRetroQ-AcGFP1-C1 Vector (2 citations) PRetroQ-EGFP-C1 (1 citations)

2 protocols using pretroq

1

Mouse Meflin cDNA Cloning and Subcloning

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cloning of mouse Meflin (mMeflin) cDNA was performed as previously described28 (link). mMeflin cDNA was subcloned into pRetroQ (Clontech, Mountain View, CA) vectors without fusion with any tag sequences (pRetroQ-mMeflin).
Minatoguchi S., Saito S., Furuhashi K., Sawa Y., Okazaki M., Shimamura Y., Kaihan A.B., Hashimoto Y., Yasuda Y., Hara A., Mizutani Y., Ando R., Kato N., Ishimoto T., Tsuboi N., Esaki N., Matsuyama M., Shiraki Y., Kobayashi H., Asai N., Enomoto A, & Maruyama S. (2022). A novel renal perivascular mesenchymal cell subset gives rise to fibroblasts distinct from classic myofibroblasts. Scientific Reports, 12, 5389.
+ Open protocol
+ Expand
2

Linx Protein Expression and Antibody Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
cDNA encoding mouse Linx (clone 6826287, GenBank accession number BC096531) was purchased from Open Biosystems and subcloned into pcDNA3.1 (Invitrogen) and pRetroQ (Clontech) vectors to fuse Linx with the V5 and SF tag, respectively. GFP-Rho-kinase cDNA was provided by M. Amano and K. Kaibuchi (Nagoya University). The following antibodies were used in this study; anti-Linx (Islr2; R&D Systems), anti-Linx (Islr2; Abnova), anti-Ret51 (IBL, Gumma, Japan), β-actin (Sigma), anti-Tau-1 (Millipore), anti-TrkA (Cell Signaling Technology), anti-phospho-MLC (Ser19; Cell Signaling Technology), anti-MLC (Cell Signaling Technology), anti-Rho-kinase 2 (ROCK2, Abcam), anti-L1 (Millipore), anti-E-cadherin (Cell Signaling Technology), anti-Na+/K+-ATPase (Abcam), and anti-GFP (MBL, Nagoya, Japan).
Abudureyimu S., Asai N., Enomoto A., Weng L., Kobayashi H., Wang X., Chen C., Mii S, & Takahashi M. (2018). Essential Role of Linx/Islr2 in the Development of the Forebrain Anterior Commissure. Scientific Reports, 8, 7292.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!