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Zscan4 antibodies

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ZSCAN4 antibodies are designed to detect the ZSCAN4 protein, which is a zinc finger and SCAN domain-containing protein that plays a role in early embryonic development. The antibodies can be used for various research applications, such as Western blotting, immunohistochemistry, and immunofluorescence. The core function of these antibodies is to specifically recognize and bind to the ZSCAN4 protein, allowing researchers to study its expression and localization in biological samples.

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Lab products found in correlation

Lamin b antibody, by Santa Cruz Biotechnology (2 mentions) Cycloheximide (chx), by Merck Group (2 mentions) β actin, by Merck Group (2 mentions)

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ZSCAN4 (4 citations)

2 protocols using zscan4 antibodies

1

Measurement of ZSCAN4 Protein Stability

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WT and doxycycline (Dox) inducible tet-ZSCAN4 Tu167 cells were treated with 1 µg/mL Dox (or kept untreated) for 24 h to induce ZSCAN4. For RNF20 knockdown, WT Tu167 cells were transfected for 48 h with either NTC-siRNA, pool RNF20 siRNA or RFN20 siRNA1–2 as described above. Cells were treated with 25 µg/ml CHX (Sigma), for the indicated time points. Total cell lysate in RIPA buffer was loaded on 10% SDS PAGE gel and immunoblotted with ZSCAN4 antibodies (1:1000; Origene) or controls, β-actin (1:1000; Millipore) and Lamin B antibodies (1:2000; Santa Cruz). Band intensities of ZSCAN4 were quantified using ImageJ software [16 (link)] and normalized to controls. The relative levels of ZSCAN4 in sample not treated with CHX was considered as initial level of ZSCAN4 and considered as 1 unit. The half-life of ZSCAN4 was determined using formula t1/2 = ln2/k (k is the slope of the degradation curve).
Portney B.A., Khatri R., Meltzer W.A., Mariano J.M, & Zalzman M. (2018). ZSCAN4 is negatively regulated by the ubiquitin-proteasome system and the E3 ubiquitin ligase RNF20. Biochemical and biophysical research communications, 498(1), 72-78.
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2

Measurement of ZSCAN4 Protein Stability

Check if the same lab product or an alternative is used in the 5 most similar protocols
WT and doxycycline (Dox) inducible tet-ZSCAN4 Tu167 cells were treated with 1 µg/mL Dox (or kept untreated) for 24 h to induce ZSCAN4. For RNF20 knockdown, WT Tu167 cells were transfected for 48 h with either NTC-siRNA, pool RNF20 siRNA or RFN20 siRNA1–2 as described above. Cells were treated with 25 µg/ml CHX (Sigma), for the indicated time points. Total cell lysate in RIPA buffer was loaded on 10% SDS PAGE gel and immunoblotted with ZSCAN4 antibodies (1:1000; Origene) or controls, β-actin (1:1000; Millipore) and Lamin B antibodies (1:2000; Santa Cruz). Band intensities of ZSCAN4 were quantified using ImageJ software [16 (link)] and normalized to controls. The relative levels of ZSCAN4 in sample not treated with CHX was considered as initial level of ZSCAN4 and considered as 1 unit. The half-life of ZSCAN4 was determined using formula t1/2 = ln2/k (k is the slope of the degradation curve).
Portney B.A., Khatri R., Meltzer W.A., Mariano J.M, & Zalzman M. (2018). ZSCAN4 is negatively regulated by the ubiquitin-proteasome system and the E3 ubiquitin ligase RNF20. Biochemical and biophysical research communications, 498(1), 72-78.
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