The following commercial primary antibodies were used: anti-phospho-ERK1/2, anti ERK1/2, anti-JNK1/2, anti-phospho-JNK1/2 and anti-phospho-c-Jun (all from Cell Signaling Technology, Danvers, MA, USA); anti-phospho-EGFR (Thermo Fisher Scientific, Rockford, IL, USA); anti-EGFR, anti-paxillin and anti-c-Jun (Santa Cruz Biotechnology, Heidelberg, Germany); and anti-β-actin (Sigma-Aldrich, St Louis, MO, USA). Secondary antibodies were anti-rabbit IgG Horseradish peroxidase-linked F(ab’)2 I fragment (from donkey) (GE Healthcare, GE, Little Chalfont, United Kingdom), anti-mouse IgG1 (BD Pharmingen, Beckton Dickinson, Franklin Lakes, NJ, USA), and Alexa Fluor 488 conjugated anti-mouse (from donkey) (Thermo Fisher Scientific, Rockford, IL, USA).
Anti rabbit igg horseradish peroxidase linked f ab 2 1 fragment from donkey
Manufactured by GE Healthcare
Sourced in United Kingdom
Anti-rabbit IgG Horseradish peroxidase-linked F(ab')2 I fragment (from donkey) is a secondary antibody reagent used for the detection of rabbit primary antibodies in various immunoassay applications. It consists of donkey-derived F(ab')2 fragments that are conjugated to the enzyme horseradish peroxidase.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin, by Merck Group (4 mentions)
Anti phospho jnk1 2, by Cell Signaling Technology (3 mentions)
Anti jnk1 2, by Cell Signaling Technology (3 mentions)
Anti phospho egfr, by Thermo Fisher Scientific (3 mentions)
Anti paxillin and anti c jun, by Santa Cruz Biotechnology (3 mentions)
Alexa fluor 488 conjugated anti mouse from donkey, by Thermo Fisher Scientific (3 mentions)
Anti phospho erk1 2, by Cell Signaling Technology (3 mentions)
Anti phospho c jun, by Cell Signaling Technology (3 mentions)
Anti erk1 2, by Cell Signaling Technology (2 mentions)
Anti egfr, by Santa Cruz Biotechnology (2 mentions)
Anti vcam 1, by Abcam (1 mentions)
Anti paxillin, by Santa Cruz Biotechnology (1 mentions)
Anti nf kb, by Abcam (1 mentions)
Anti phospho nf κb, by Cell Signaling Technology (1 mentions)
Anti erk, by Cell Signaling Technology (1 mentions)
Alexa fluor 488 conjugated anti mouse, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 conjugated anti rabbit, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 594 conjugated anti mouse, by Thermo Fisher Scientific (1 mentions)
Anti egfr, by Thermo Fisher Scientific (1 mentions)
Anti phospho fak, by Abcam (1 mentions)
4 protocols using anti rabbit igg horseradish peroxidase linked f ab 2 1 fragment from donkey
1
Antibody Staining for Signaling Proteins
2
Antibody Staining for Signaling Proteins
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The following commercial primary antibodies were used: anti-phospho-ERK1/2, anti ERK1/2, anti-JNK1/2, anti-phospho-JNK1/2 and anti-phospho-c-Jun (all from Cell Signaling Technology, Danvers, MA, USA); anti-phospho-EGFR (Thermo Fisher Scientific, Rockford, IL, USA); anti-EGFR, anti-paxillin and anti-c-Jun (Santa Cruz Biotechnology, Heidelberg, Germany); and anti-β-actin (Sigma-Aldrich, St Louis, MO, USA). Secondary antibodies were anti-rabbit IgG Horseradish peroxidase-linked F(ab’)2 I fragment (from donkey) (GE Healthcare, GE, Little Chalfont, United Kingdom), anti-mouse IgG1 (BD Pharmingen, Beckton Dickinson, Franklin Lakes, NJ, USA), and Alexa Fluor 488 conjugated anti-mouse (from donkey) (Thermo Fisher Scientific, Rockford, IL, USA).
3
Antibody Characterization in Cell Signaling
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The following commercial primary antibodies were used: 1:1,000 anti‐phospho‐NF‐κB (Cell Signaling Technology, Danvers, MA, USA); 1:1,000 anti‐NF‐kB and 1:1,000 anti‐VCAM1 (Abcam, Cambridge, United Kingdom); 1:200 anti‐paxillin (Santa Cruz Biotechnology, Heidelberg, Germany); and 1:4,000 anti‐β‐actin (Sigma‐Aldrich, St Louis, MO, USA). Secondary antibodies were as follows: 1:1,000 anti‐rabbit IgG Horseradish peroxidase linked F(ab’)2 I fragment (from donkey) (GE Healthcare, GE, Little Chalfont, United Kingdom); 1:3,000 anti‐mouse IgG1 (BD Pharmingen, Beckton Dickinson, Franklin Lakes, NJ, USA); and 1:400 Alexa Fluor 488 conjugated anti‐mouse (from donkey) (Thermo Fisher Scientific, Rockford, IL, USA).
4
Antibody Detection in Cell Signaling
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The following commercial primary antibodies were used: anti-phospho-ERK1/2, anti-ERK, anti-JNK1/2, anti-phospho-JNK1/2, and anti-phospho-c-Jun (all from Cell Signaling Technology, Danvers, MA, USA); anti-phospho-EGFR, anti-EGFR, anti-FAK (all from Thermo Fisher Scientific, Rockford, IL, USA); anti-phospho-FAK (Abcam, Cambridge, MA, USA); anti-paxillin and anti-c-Jun (Santa Cruz Biotechnology, Heidelberg, Germany); anti-phospho-paxillin Ser 178 (Antibodies-online GmbH, Aachen, Germany); and anti-β-actin (Sigma-Aldrich, St Louis, MO, USA). Secondary antibodies were anti-rabbit IgG Horseradish peroxidase linked F(ab')2 I fragment (from donkey) (GE Healthcare, GE, Little Chalfont, United Kingdom), anti-mouse IgG1 (BD Pharmingen, Beckton Dickinson, Franklin Lakes, NJ, USA); Alexa Fluor 488 conjugated anti-rabbit (from donkey), Alexa Fluor 488 conjugated anti-mouse (from donkey) and Alexa Fluor 594 conjugated anti-mouse (from donkey) (all from Thermo Fisher Scientific, Rockford, IL, USA).
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