Methanol HPLC grade was purchased from Sigma-Aldrich (St. Louis, MO, United States). Melphalan (compound 1 ) and phenylalanine (compound 2 ) were purchased from J&K Scientific Ltd (Shanghai, China) with a purity>99%. 3,4-Dimethoxyphenylalanine (compound 3 ), 4-chlorophenylalanine (compound 4 ), 4-methoxyphenylalanine (compound 5 ), 4-methylphenylalanine (compound 6 ), tyrosine (compound 7 ), 4-bromophenylalanine (compound 8 ) and 2-hydroxylbenzylamine were purchased from Sinopharm Chemical Reagent Co., Ltd. Their structures of them are listed in Scheme 1 . Experimental ultrapure water produced by Millipore ultrapure water system.
Tyrosine
Manufactured by Sinopharm
Sourced in China
Tyrosine is an amino acid used in the production of various laboratory equipment. It serves as a key component in the manufacturing process of these products.
Automatically generated - may contain errors
Lab products found in correlation
Tryptophan, by Sinopharm (2 mentions)
Phenylalanine, by Sinopharm (2 mentions)
Chitosan, by Sinopharm (2 mentions)
Calcium chloride, by Sinopharm (2 mentions)
Casein, by Sinopharm (2 mentions)
Hplc grade methanol, by Merck Group (1 mentions)
Phenylalanine, by J&K Scientific (1 mentions)
Glutamic acid, by Sinopharm (1 mentions)
Threonine, by Sinopharm (1 mentions)
Histidine, by Sinopharm (1 mentions)
Aspartic acid, by Sinopharm (1 mentions)
Leucine, by Sinopharm (1 mentions)
Valine, by Sinopharm (1 mentions)
Proline, by Sinopharm (1 mentions)
Sephadex g 15, by Merck Group (1 mentions)
Glacial acetic acid, by Sinopharm (1 mentions)
Triethylamine, by Sinopharm (1 mentions)
Glycine, by Sinopharm (1 mentions)
6 protocols using tyrosine
1
Synthesis and characterization of phenylalanine derivatives
2
Azalomycin F Interaction with Amino Acids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Eight amino acids including tyrosine, tryptophan, threonine, glutamic acid, aspartic acid, histidine, phenylalanine, and arginine (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China) were added into a 30% methanol aqueous solution of azalomycin F (100 μM) to obtain a mixed solution by vortexing, according to the molar concentration ratio of azalomycin F to each amino acid of 1:8. Simultaneously, the 30% methanol aqueous solution that only contained 100 μM azalomycin F was prepared as a blank control for background subtraction. After being incubated in a water bath at 37 °C for 30 min, these solutions were analyzed by a TripTOF 5600+ mass spectrometry (AB Sciex, Framingham, MA, USA) with an electrospray ion resource, and the detailed parameters are shown in Table S3 for negative ionization modes.
3
Extraction and Analysis of Bioactive Compounds from Sea Cucumber
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sea cucumber (Acaudina molpadioidea) was purchased from a local market (Qingdao, China) and its water content was determined to be 12.65% per 20 g on average. Hippuryl histidyl leucine (HHL), rabbit-lung ACE and Sephadex G-15 were purchased from Sigma Chemical Ltd (St. Louis, MO, USA). Papain (from papaya) and trypsin (from bovine sources) were purchased from Nanning Pangbo Biological Engineering Ltd (Nanning, China). O-Pthaldialdehyde (OPA) was purchased from Beijing Soularbio Technology Ltd. (Beijing, China). Proline (PubChem CID 145742), valine (PubChem CID 6287), tyrosine (PubChem CID 6057), tryptophan (PubChem CID 6305), leucine (PubChem CID 6106) and phenylalanine (PubChem CID 6140) were purchased from Sinopharm Chemical Reagent Ltd. (Shanghai, China). All other chemicals were obtained from local commercial sources and were of the highest purity available.
4
Characterization of Biomaterial Composites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Unico uv2100 ultraviolet spectrophotometer was purchased from Beijing Boya Innovation Technology Co., Ltd to determine the concentration of protein. High-resolution X-ray D8Discover25 Cuk α radiation source, tube voltage 40KV, tube current 500mA, scanning range 3 °-40 °to determine the crystal structure of materials. Fourier transform infrared spectrometer VERTEX70, KBr compression method, scanning range 4000-500cm -1 was studied the structure features of sodium alginate (SA), chitosan(CA)，and sodium alginatechitosan(SA-CA). Scanning electron microscope JSM-6710f of cold field emission type, image resolution: 1.0nm (15KV), 2.2nm (1KV), magnification: X25-65000, accelerating voltage: 0.5KV-30KV was used to observe the morphology of materials.
The chemicals used in this study were of analytical grade. SA, chitosan, calcium chloride, casein, tyrosine were purchased from the Sinopharm Chemical Reagent Co, Ltd, China.
The chemicals used in this study were of analytical grade. SA, chitosan, calcium chloride, casein, tyrosine were purchased from the Sinopharm Chemical Reagent Co, Ltd, China.
5
Isolation and Characterization of Lactic Acid Bacteria
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Streptococcus thermophilus ABT-T and Lactobacillus delbrueckii ssp. bulgaricus BNCC 336436 were purchased from American Type Culture Collection (Manassas, VT). Levilactobacillus brevis CGMCC1.5954 was screened by our laboratory and stored in the China General Microbiological Culture Collection Center (Beijing, China). Methanol, acetonitrile, and anhydrous sodium acetate were purchased from Maclean's MACKLIN, Tedia, and Aladdin Biochemical Ltd., respectively. The sucrose and milk powder were provided by Angie Yeast Co. and Apatamil. Glycine, tyrosine, phenyl isothionitrate, triethylamine, glacial acetic acid, sodium l-glutamate (L-MSG), and hexane were all manufactured by Sinopharm Chemical Reagent Co.
6
Characterization of Biomaterial Composites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Unico uv2100 ultraviolet spectrophotometer was purchased from Beijing Boya Innovation Technology Co., Ltd to determine the concentration of protein. High-resolution X-ray D8Discover25 Cuk α radiation source, tube voltage 40KV, tube current 500mA, scanning range 3 °-40 °to determine the crystal structure of materials. Fourier transform infrared spectrometer VERTEX70, KBr compression method, scanning range 4000-500cm -1 was studied the structure features of sodium alginate (SA), chitosan(CA)，and sodium alginatechitosan(SA-CA). Scanning electron microscope JSM-6710f of cold field emission type, image resolution: 1.0nm (15KV), 2.2nm (1KV), magnification: X25-65000, accelerating voltage: 0.5KV-30KV was used to observe the morphology of materials.
The chemicals used in this study were of analytical grade. SA, chitosan, calcium chloride, casein, tyrosine were purchased from the Sinopharm Chemical Reagent Co, Ltd, China.
The chemicals used in this study were of analytical grade. SA, chitosan, calcium chloride, casein, tyrosine were purchased from the Sinopharm Chemical Reagent Co, Ltd, China.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!