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Horseradish peroxidase hrp linked anti rabbit igg pab

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Horseradish peroxidase (HRP)-linked anti-rabbit IgG (pAb) is a secondary antibody conjugated with the enzyme horseradish peroxidase. It is used to detect and visualize primary antibodies that are raised in rabbit.

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Lab products found in correlation

Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions) Streptavidin hrp, by R&D Systems (1 mentions) Rabbit anti hmg1 2 3 pab, by Santa Cruz Biotechnology (1 mentions) Biotinylated anti human igg pab, by Southern Biotech (1 mentions) Protein a g plus agarose, by Santa Cruz Biotechnology (1 mentions) Femto chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) Mini protean tgx precast gradient gel, by Bio-Rad (1 mentions) Protease inhibitor cocktail 5, by Merck Group (1 mentions) Hrp linked goat anti actin, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Horseradish peroxidase anti-rabbit Horseradish peroxidase (HRP) Rabbit pAb Anti-HRP rabbit Horseradish peroxidase Rabbit anti-IgG IgG rabbit

2 protocols using horseradish peroxidase hrp linked anti rabbit igg pab

1

Immunoprecipitation and Immunoblotting of Tim-3

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We added 5μg of recombinant (r) mouse Tim-3 human immunoglobulin G (IgG)1 chimeric protein (rTim-3Fc; R&D Systems, Minneapolis, MN) to 500 μL of supernatant and immunoprecipitated with Protein A/G PLUS-Agarose (Santa Cruz Biotechnology, Dallas, TX). Proteins were resolved, western blotted, and incubated with rabbit anti-HMG1/2/3 (pAb; Santa Cruz Biotechnology), biotinylated anti-human IgG (pAb; SouthernBiotech, Birmingham, AL), horseradish peroxidase (HRP)-linked anti-rabbit IgG (pAb; Cell Signaling Technology, Danvers, MA), and streptavidin-HRP (R&D Systems), followed by visualization with SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, Rochester, NY).
Dolina J.S., Braciale T.J, & Hahn Y.S. (2014). Liver-primed CD8+ T cells suppress antiviral adaptive immunity through galectin-9-independent T-Cell immunoglobulin and mucin 3 engagement of high-mobility group box 1 in mice. Hepatology (Baltimore, Md.), 59(4), 1351-1365.
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2

Phosphorylation of mTOR and MERTK

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Cultured cells were lysed in buffer containing NaF, Na4P2O7, Na3VO4, and protease inhibitor cocktail V (EMD Millipore, Temecula, CA) to preserve protein phosphorylation. Proteins were resolved on Mini-PROTEAN TGX precast gradient gels (BioRad, Berkeley, CA), transferred to PVDF membranes, and incubated with rabbit anti-phospho-mTOR (mAb, Cell Signaling Technology, Danvers, MA), anti-mTOR (mAb, Cell Signaling Technology), anti-phospho-MERTK (pAb, FabGennix, Frisco, TX), or anti-MERTK (pAb, FabGennix). The blots were then incubated with horseradish peroxidase (HRP)-linked anti-rabbit IgG (pAb, Cell Signaling Technology) and HRP-linked goat anti-Actin (Santa Cruz Biotechnology, Santa Cruz, CA) and visualized with an 80/20 mix of SuperSignal West Pico Chemiluminescent Substrate and Femto Chemiluminescent Substrate (Thermo Scientific, Rochester, NY). Densitometry was performed using ImageJ analysis software (National Institute of Health, Bethesda, MD).
Labonte A.C., Sung S.J., Jennelle L.T., Dandekar A.P, & Hahn Y.S. (2016). Expression of scavenger receptor‐AI promotes alternative activation of murine macrophages to limit hepatic inflammation and fibrosis. Hepatology (Baltimore, Md.), 65(1), 32-43.
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