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Sybr green luna one step rt qpcr kit

Manufactured by New England Biolabs
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The SYBR green Luna One Step RT-qPCR Kit is a reagent for performing one-step reverse transcription and quantitative PCR (RT-qPCR) reactions. It contains a DNA polymerase, reverse transcriptase, and SYBR Green I dye for the detection and quantification of RNA targets.

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Lab products found in correlation

C1000 touch, by Bio-Rad (2 mentions) Quantstudio 6, by Thermo Fisher Scientific (2 mentions) Rneasy plus mini kit, by Qiagen (2 mentions)

Spelling variants of this product

SYBR Green (10 citations) Luna one-step RT-qPCR kit (5 citations) SYBR Green qPCR kit (3 citations) Sybr green Luna Universal one-step RT-qPCR kit (3 citations)

2 protocols using sybr green luna one step rt qpcr kit

1

Quantifying mRNA Expression Using RT-qPCR

Cited 1 time
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RNA was isolated from cells using the RNeasy Plus Mini Kit (Qiagen). mRNA expression was determined using SYBR green Luna One Step RT-qPCR Kit (New England BioLabs) on a C1000 Touch (Bio-Rad) or QuantStudio 6 (Applied Biosystems) thermocycler. Expression was quantified by the ΔΔCt method using target-specific and control primers. β-2-microglobulin (B2M) used as an internal control. Primer sequences: B2M Fwd 5’-GGCATTCCTGAAGCTGACA-3, Rev 5’-CTTCAATGTCGGATGGATGAAAC-3’, CLDN4 Fwd 5’-GCCTTACTCCGCCAAGTATT-3’, Rev 5- AGGGAAGAACAAAGCAGAGAG-3’ [15 (link)].
Yamamoto T.M., Webb P.G., Davis D.M., Baumgartner H.K., Woodruff E.R., Guntupalli S.R., Neville M., Behbakht K, & Bitler B.G. (2022). Loss of Claudin-4 reduces DNA damage repair and increases sensitivity to PARP inhibitors. Molecular cancer therapeutics, 21(4), 647-657.
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2

Quantification of mRNA Expression via RT-qPCR

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was isolated from cells using the RNeasy Plus Mini Kit (Qiagen). mRNA expression was determined using SYBR green Luna One Step RT-qPCR Kit (New England BioLabs) on a C1000 Touch (Bio-Rad) or QuantStudio 6 (Applied Biosystems) thermocycler. Expression was quantified by the ΔΔCt method using target-specific and control primers. β-2-microglobulin (B2M) was used as internal control. mRNA-specific primers were designed to span exon-exon junctions to avoid detection of genomic DNA. Primer sequences: TNF-F:
Johnson J., Bales E.S., Bitler B.G., & Watson Z.L. (2020). Eliciting OTUD3/RIPK-Dependent Necroptosis to Prevent Epithelial Ovarian Cancer.
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