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4 protocols using niflumic acid

1

Analyzing Cell Proliferation Signaling

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BrdU, mouse anti-BrdU antibody, PI, L-161,982 and celecoxib were purchased from Sigma-Aldrich. 16,16-dimethyl-PGE2, XAV939, AH6809 and forskolin were from Tocris Bioscience (Bristol, UK). Niflumic acid was from Cayman Chemical (Ann Arbor, MI, USA). All other chemicals were of analytical grade.
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2

Screening of 56 Antischistosomal Compounds

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A total of 56 compounds (Figure S4, Table S4) was selected by both the manual and automated methods for screening
of S. mansoni adults and somules. The following
compounds were purchased from Cayman Chemical: U-73122, BIX01294 hydrochloride
hydrate, tyrphostin AG-1478, sivelestat sodium tetrahydrate, AGK2,
trilostane, itraconazole, dabigatran etexilate, SB202190 hydrochloride,
niflumic acid, amsacrine hydrochloride, R(+)-IAA-94,
piperlongumine, tiamulin fumarate, PNU-282987, tetrabenazine, nemadipine-A,
mycophenolic acid, etravirine, (S)-duloxetine hydrochloride,
moxidectin, and rutecarpine. The following compounds were purchased
from Sigma-Aldrich: clindamycin 2-phosphate, tyrphostin I-OMe-AG-538,
and escabet sodium. The following compounds were purchased from Selleck
Chemicals: revaprazan hydrochloride and Org 27569. The following compounds
were purchased from Enamine: Z385159220, Z18885599, Z276431168, Z2241105867,
Z827016000, Z48867676, Z53005631, Z90192490, Z56174662, Z105384660,
Z44528364, Z827015296, Z304863612, and Z56958732. Finally, the following
compounds were purchased from Toronto Research Chemical: eletriptan
and ondansetron hydrochloride dihydrate. Powders were stored according
to vendor specifications. Compounds were then dissolved at 10 mM in
fresh DMSO and shipped to UCSD on dry ice for storage at −80
°C until use.
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3

Neurophysiology Experiments with Pharmacological Agents

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Drugs and dyes used for experiments are listed in final concentration (in mM) and were purchased from the following suppliers: 0.2 furosemide, 0.1 niflumic acid (Cayman Chemicals), 0.05 picrotoxin, 0.02 NMDA, 0.01 tributyltin, all salts for aCSF and slicing solution (Sigma-Aldrich), 0.01 Glycine (EMD Chemicals), 0.0001 Apamin, 0.1 NPPB, 0.05 bumetanide, 0.05 VU0240551, 0.5 Rubi-GABA Trimethylphosphine, and 0.01 nigericin (Tocris), 0.0005 TTX (AbCam), 0.05 Alexa-594 hydrazide, 0.6-6 MQAE, and 2 mg/mL Biocytin Alexa Fluor 488 (Thermo Fisher Scientific). All drugs were made into aliquots dissolved in water or DMSO and diluted to the final concentration in aCSF or intracellular recording solution -the final concentration of DMSO never exceeded 0.1%.
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4

Diverse Neurochemical Agents in Experiments

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Drugs and dyes used for experiments are listed in final concentration (in mM) and were purchased from the following suppliers: 0.2 furosemide, 0.1 niflumic acid (Cayman Chemicals), 0.05 bumetanide, 0.05 picrotoxin, 0.02 NMDA, 0.01 tributyltin, all salts for aCSF and slicing solution (Sigma-Aldrich), 0.01 Glycine (EMD Chemicals), 0.1 NPPB, 0.05 bumetanide, and 0.01 nigericin (Tocris), 0.05 Alexa-594 hydrazide, 0.6-8 MQAE (Thermo Fisher Scientific). All drugs were made into aliquots dissolved in water or DMSO and diluted to the final concentration in aCSF or intracellular recording solution -the final concentration of DMSO never exceeded 0.1%.
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