Glomax discover plates reader

UDSCC2 cell line was transfected with specific siRNA against SMAD4 or Luciferase as control. 24 h after transfection, cells were seeded into 96well plates (7000 cells/well) and 24 h later were treated with 10uM Cisplatin for 24 h. Cell viability was then assessed using CellTiter-Glo Luminescent Cell Viability Assay and luminescence was measured using a Glomax Discover plates reader (Promega).

UD-SCC-2, UM-SCC-4, UM-SCC-19 and UPCI-SCC-152 cell lines were seeded into 96-well white plates (1500 or 2000 cells/well) and 24 h later were treated with 9 different concentrations of cisplatin (Teva) for 72 h and vehicle, alone or in combination with 1 µM JQ-1 (Medchem Express) or 1 µM DT-061 (Medchem Express). UD-SCC-2 cell lines transduced with shluc, shMYC#1, shMYC#2, shCIP2A#1, shCIP2A#2 were seeded into 96-well white plates (2000 cells/well) and 24 h later were treated with 9 different concentrations of cisplatin (Teva) for 72 h. Cell viability was then assessed using CellTiter-Glo Luminescent Cell Viability Assay (Promega) following manufacturer instructions and luminescence was measured using a Glomax Discover plates reader (Promega).