Prism for windows

For organ weight data in toxicity study, analysis of covariance with Student’s t test was performed using terminal body weight as covariate [42 (link)], unless non-parametric methods were applied. The treatment comparisons were made on adjusted group means in order to allow for differences in body weight that might influence the organ weights. Significant differences between the groups compared were calculated using GraphPad Prism for Windows version 5.03, GraphPad Software, San Diego, CA, USA, and expressed as * p < 0.05.
Regarding efficacy study, data were expressed as means ± standard errors of the means. Statistical analysis was used to determine whether there were significant differences in BAL total and differential cell counts and BAL cytokine levels between SET-M33 (0.5, 2 and 5 mg/kg) or budesonide (1 mg/kg)-treated LPS-challenged animals and vehicle-treated LPS-challenged animals. Statistical analysis was also performed to compare vehicle-treated LPS-challenged animals with vehicle-treated saline-challenged animals. Statistical analysis was performed using the Student’s t-test with GraphPad Prism for Windows version 5.03, GraphPad Software, San Diego, CA, USA. The levels of statistical significance are indicated in the legends of the figures.

The data from each experiment are presented as mean ± standard error of the mean (SEM) and were analyzed by Prism for Windows (version 8.0, GraphPad Software Inc., USA). Multiple comparisons were performed by one-way analysis of variance (ANOVA) with Newman–Keuls posttests. P-values less than 0.05 were considered to be statistically significant.

All data are expressed as means ± standard deviation. For whole‐cell patch‐clamp electrophysiology data, the sample sizes (n) indicate the numbers of cells recorded and mice (i.e., total number of cells recorded/total number of mice used). Statistical analyses were performed using Prism for Windows (version 9; GraphPad Software, La Jolla, CA) and OriginPro (version 7.5, OriginLab, Northampton, MA). The D’Agostino‐Pearson test was used to test the normality of all electrophysiology and echocardiography data and the distributions were found to be normal in all cases except for the APD₅₀ values with DTX‐K in WT mice and the echocardiographic measurements of LVPWd in WT mice and LVAWd in KO mice. For comparisons involving two groups, either paired or unpaired two‐tailed Student's t‐tests were employed as appropriate. The action potential duration data were also analyzed using nested (i.e., hierarchical) t‐tests and no significant differences were identified between animals of the same genotype.

Results are expressed as means ± SEM. We applied two-way ANOVA (with Tukey’s correction for comparison of multiple means) for comparisons of all parameters as the experimental setup contained two variables per group (noise exposure and drug treatment). We used Prism for Windows, version 8.1, GraphPad Software Inc., for statistical analyses. p-Values < 0.05 were considered statistically significant, and symbols of significance are explained in the figure legends.