Image pro plus

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Image-Pro Plus is a comprehensive software solution for advanced image analysis and processing. It provides a suite of tools for image capture, enhancement, measurement, and analysis. The software is designed to work with a wide range of microscopy, imaging, and digital photography equipment, making it a versatile tool for researchers, scientists, and professionals in various fields.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Merck Group (13 mentions) Fluorescence microscope, by Olympus (12 mentions) Bx51 microscope, by Olympus (11 mentions) Bca protein assay kit, by Thermo Fisher Scientific (10 mentions) Pvdf membrane, by Merck Group (10 mentions) Microtome, by Leica (8 mentions) Eclipse 80i, by Nikon (8 mentions) Oil red o, by Merck Group (7 mentions) In situ cell death detection kit, by Roche (7 mentions) Hoechst 33342, by Merck Group (6 mentions) Retrievagen a, by Zymo Research (6 mentions) Spot flex camera, by Olympus (6 mentions) Fluorescence microscope, by Leica (6 mentions) Matrigel, by BD (6 mentions) Live dead baclight bacterial viability kit, by Thermo Fisher Scientific (6 mentions) Bx600 microscope, by Olympus (6 mentions) Prism 5, by GraphPad (6 mentions) Buffered natural formaldehyde, by Guangzhou Chemical (6 mentions) Masson s trichrome, by Merck Group (5 mentions) Triphenyl tetrazolium chloride (ttc), by Merck Group (5 mentions)

1 608 protocols using image pro plus

Hippocampal Cell Counting and Protein Analysis

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The numbers of NeuN-positive and DCX-positive cells were counted hemilaterally under a light microscope (Olympus, Tokyo, Japan) and expressed as the numbers of cells per mm² in the hippocampal dentate gyrus. The numbers of Fluro-Jade B-positive, TUNEL-positive, and caspase-3-positive cells were counted hemilaterally under a light microscope (Olympus) and expressed as the numbers of cells per 320×100 μm hippocampal CA1 region. The selected hippocampal area was measured by use of the Image-Pro Plus computer-assisted image analysis system (Media Cybernetics Inc., Silver Spring, MD, USA). To compare the relative expressions of Bax and Bcl-2, the detected bands were calculated densitometrically using Image-Pro Plus software (Media Cybernetics Inc.).
All data were analyzed using IBM SPSS Statistics ver. 21.0 (IBM Co., Armonk, NY, USA). The data were expressed as the mean± standard error of the mean. For the comparison among the groups, one-way analysis of variance and Duncan post hoc test were performed with P<0.05 as an indication of statistical significance.

Lee J.M., Ji E.S., Kim T.W., Kim C.J., Shin M.S., Lim B.V., Chung Y.R, & Cho Y.S. (2018). Treadmill exercise improves memory function by inhibiting hippocampal apoptosis in pilocarpine-induced epileptic rats. Journal of Exercise Rehabilitation, 14(5), 713-723.

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Quantifying Bone Formation, Resorption, and Marrow Adiposity

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The tibia sections were stained with hematoxylin and eosin (H&E), Oil red O, and Mayer’s hematoxylin and histochemically for alkaline phosphatase (ALP) activity using BCIP/NBT kit (Beyotime Biotechnology, China) and tartrate-resistant acid phosphatase (TRAP) activity using TRACP kit (Sigma, USA), respectively. The sections were then counterstained with methyl green and mounted in Kaiser’s glycerol jelly. The following parameters were measured: the ALP-positive osteoblast surface per bone surface (OB.S/BS, %) for bone formation, the TRAP-positive osteoclast surface per bone surface (OC.S/BS, %) for bone resorption, and the adipocyte area per bone marrow area without trabecula (%) for marrow adiposity [16 (link)]. Images of micrographs from single sections were digitally recorded using a rectangular template, and recordings were processed and analyzed using Image Pro Plus image analysis software (Image Pro Plus, version 4.112, Media Cybernetics, LP, USA).

Zou Q., Hong W., Zhou Y., Ding Q., Wang J., Jin W., Gao J., Hua G, & Xu X. (2016). Bone marrow stem cell dysfunction in radiation-induced abscopal bone loss. Journal of Orthopaedic Surgery and Research, 11, 3.

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Immunocytochemical Analysis of Protein Expression

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Cells were fixed in 4% paraformaldehyde (Sigma) for 15 min at RT, rinsed 3 times with PBS (Fisher), and then incubated with permeabilizing/blocking buffer containing 5% goat serum (Vector Laboratories) and 0.2% Triton X-100 (Bio-Rad) in PBS for 60 min at RT. Primary antibodies were added overnight at 4 °C. The following day cells were washed 3 times with PBS and incubated with secondary antibodies (Molecular Probes) for 60 min at RT. Cells were counterstained with DAPI (Sigma-Aldrich). IgG control was used as negative controls for all immunocytochemical analysis. Images were captured using a Nikon Eclipse E800 microscope equipped with a digital imaging system and imported into Image-ProPlus, version 7.0 (Media Cybernetics, Sliver Spring, MD) for quantification. Images were imported into Image-Pro Plus, version 7.0 (Media Cybernetics, Silver Spring, MD), for quantification and 600–1,000 immunostained cells from 15 random fields per group were counted.

Ma K., Deng X., Xia X., Fan Z., Qi X., Wang Y., Li Y., Ma Y., Chen Q., Peng H., Ding J., Li C., Huang Y., Tian C, & Zheng J.C. (2018). Direct conversion of mouse astrocytes into neural progenitor cells and specific lineages of neurons. Translational Neurodegeneration, 7, 29.

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Histological Analysis of Plant Tissues

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The material collected was processed according to Travaglia et al. (2012) . Micro-sections (10 μm) of leaf midrib and berry pedicels (at the berry proximal zone) were prepared using a rotary microtome, whereas stem cross-sections were prepared by hand-cut. The histological preparations were photographed with a camera (AxioCam HRc camera, Carl Zeiss, Göttingen, Germany) attached to a standard microscope (Model 16, Carl Zeiss) . The boundary between xylem and phloem was easily identified because of the differential staining of the cell walls corresponding to sieve and vessel elements. The tissue area was calculated using the software Image Pro-Plus (Media Cybernetics Inc, Rockville, MD). Stomata density was assessed by taking two imprints of the abaxial surface of a fully expanded leaf (13th from the shoot apex) with transparent nail varnish. Imprints were performed in middle zone between the main midrib and the leaf margin. When imprints dried, they were mounted onto a slide for examination under optical microscope (40×). Three representative photographs per imprint were taken using an AxioCam HRc camera attached to a Zeiss Axiophot (Carl Zeiss) microscope. Stomata density was calculated as the mean value of the number of stomata per square millimeter of six photographs per leaf. The Image Pro-Plus software (Media Cybernetics Inc) was used to analyze the microphotographs.

Murcia G., Pontin M., Reinoso H., Baraldi R., Bertazza G., Gómez-Talquenca S., Bottini R, & Piccoli P.N. (2016). ABA and GA3 increase carbon allocation in different organs of grapevine plants by inducing accumulation of non-structural carbohydrates in leaves, enhancement of phloem area and expression of sugar transporters. Physiologia plantarum, 156(3).

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Histomorphometric Analysis of Bone-Implant Interface

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After conventional light-microscopical examination, histomorphometric measurements were made using an automated two-image analysis system Image-Pro Plus (Image-Pro Plus, Media Cybernetics, Silver Spring, MD) and IMT i-Solution Lite (IMT i-Solution Lite ver 8.1, IMT i- Solution. Inc, Canada). After taking a ×12.5 enlarged photograph of the whole tissue specimen, each part required for measurement was measured by ×50 and ×100 magnifications, and various images were taken from one specimen with no overlapping. Bone-to-implant contact, as well as bone healing, defined as the linear length of bone surface in direct contact with implant divided by the implant perimeter starting from the most coronal surface-treated position down to a range of 2.1- to 2.7-mm distance along the implant surface (Fig. 2), was then calculated.Fig. 2

Bone-to-implant contact. The histological micrograph shows the position subjected to BIC measurement

Peng W., Kim I.K., Cho H.Y., Seo J.H., Lee D.H., Jang J.M, & Park S.H. (2016). The healing effect of platelet-rich plasma on xenograft in peri-implant bone defects in rabbits. Maxillofacial Plastic and Reconstructive Surgery, 38(1), 16.

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Histological Analysis of Bone Regeneration

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The histological analysis was conducted according to our previous study⁴¹. Briefly, the animals were euthanized 12th-week post-operationand the harvested tissues were fixed in neutral buffered formalin (NBF, 10%; pH 7.26) for 48 h. The treated bones were decalcified by storage in nitric acid (5% v/v) for 10 days. The decalcified bones then went through histological fixation and dehydration processes, embedded in paraffin. Sections (5 µm thick) were cut and stained with Haematoxylin and Eosin (H&E) and Masson's trichrome (MCT). The resulted histological slides were evaluated by the independent pathologist, using light microscopy (Olympus BX51; Olympus, Tokyo, Japan). The amount of the newly formed cartilage or bone was assessed as well as the amount of the remaining implants in the total area of the section. Furthermore, the histomorphometric analysis was carried out and the appearance and number of different cells including; fibroblasts, fibrocytes, chondroblasts, chondrocytes, osteoblasts, osteocytes, giant cells, macrophages, lymphocytes, neutrophils, as well as other constituents such as blood vessels, and new cartilage and bone tissues were studied. The resulted data were analyzed using Image-Pro Plus (version 6, Media Cybernetics, Inc., https://www.mediacy.com/imageproplus, Silver Spring, USA).

Samadian H., Farzamfar S., Vaez A., Ehterami A., Bit A., Alam M., Goodarzi A., Darya G, & Salehi M. (2020). A tailored polylactic acid/polycaprolactone biodegradable and bioactive 3D porous scaffold containing gelatin nanofibers and Taurine for bone regeneration. Scientific Reports, 10, 13366.

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Histological Analysis of Bone Formation and Resorption

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Tibia sections were stained with Oil Red O and Mayer’s haematoxylin and histochemically tested for alkaline phosphatase (ALP) activity using a BCIP/NBT kit (Beyotime Biotechnology, Jiangsu, China) and for tartrate-resistant acid phosphatase (TRAP) activity using a TRACP kit (Sigma-Aldrich, St. Louis, MO, USA). The sections were then counterstained with methyl green and mounted in Kaiser's glycerol jelly. The following parameters were measured: the ALP-positive osteoblast surface per bone surface (OB.S/BS, %) for bone formation, the TRAP-positive osteoclast surface per bone surface (OC.S/BS, %) for bone resorption, and the adipocyte area per bone marrow area without trabecula (%) for marrow adiposity [18 (link)]. Images of micrographs from single sections were digitally recorded using a rectangular template, and recordings were processed and analysed using Image-Pro Plus image analysis software (Image-Pro Plus, version 4.112, Media Cybernetics, LP, Silver Spring, MD, USA).

Xu X., Li R., Zhou Y., Zou Q., Ding Q., Wang J., Jin W., Hua G, & Gao J. (2017). Dysregulated systemic lymphocytes affect the balance of osteogenic/adipogenic differentiation of bone mesenchymal stem cells after local irradiation. Stem Cell Research & Therapy, 8, 71.

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Automated Brn3a+ Cell Counting in Chicken Retinas

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Every quadrant of the retina was analyzed separately. The automated Brn3a+ cell counting routines for Image-Pro Plus (Image-Pro Plus, IPP 5.1 for Windows; Media Cybernetics, Silver Spring, MD) for rat [24 (link), 40 (link), 41 (link)] and mouse retinas [25 (link), 42 (link)] were adapted to the analysis of chicken retinas. The chicken retinas are larger and contain in the order of 50 times more RGCs than a rat retina. Images were orderly selected from the reconstructed quadrant and converted to 16-bit gray scale, followed by the application of the higauss 7x7 enhancement filter. The resulting images were then filtered through a large spectral filter: Edge+, which extract positive edges from the dark background. Potential cell clusters were separated by the IPP watershed split morphologic filter. Cells were counted within predetermined parameters to exclude objects that are too large and too small to be the RGCs nuclei. Finally, data of each count were displayed and exported to a spreadsheet where they were filed and saved for further analysis. The total number of Brn3a⁺RGCs was obtained as the sum of the four quadrants of each retina.

Galindo-Romero C., Harun-Or-Rashid M., Jiménez-López M., Vidal-Sanz M., Agudo-Barriuso M, & Hallböök F. (2016). Neuroprotection by α2-Adrenergic Receptor Stimulation after Excitotoxic Retinal Injury: A Study of the Total Population of Retinal Ganglion Cells and Their Distribution in the Chicken Retina. PLoS ONE, 11(9), e0161862.

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Quantitative Analysis of Aortic Lipid Deposition

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En-face Oil red O staining: aortas of two mice from each group were separated and fixed for 24 h. The vessels were cut longitudinally along the vessel wall and stained with Oil red O liquid for 60 min at 37°C. The vessels were then incubated with 75% ethanol until the atherosclerotic lesions turned red and the vessel wall became white. Cross section Oil red O staining: Aortic roots from six mice embedded in OCT were serially cross sectioned (5 μm thick sections) and stained with Oil red O (G1261-2, Solarbio, China) to evaluate the lesion size and lipid contents. After circling the outline of blood vessels (including whole valves), Image pro plus (Ver. 6.0, Media Cybernetics, MD, United States) was used to calculate the total area of blood vessels (including positive staining area) based on total area, and then the positive area is calculated based on Oil red O under the same contour, and finally the positive area/total area of blood vessels is calculated. Images were captured by Pannoramic DESK (3DHISTECH, Hungary) and preprocessed by CaseViewer (Ver. 2.4, 3DHISTECH, Hungary) and analyzed by Image-Pro Plus (Ver. 6.0, Media Cybernetics, MD, United States).

Chen J., Li K., Shao J., Lai Z., Gao R., Wang C., Song X., Guo W., Yu X., Du F., Zhu Z., Wang J., Ma J., Xu L., Zhou Y., Liu J., Shu K., Zhao H., Wang J, & Liu B. (2022). Irisin Suppresses Nicotine-Mediated Atherosclerosis by Attenuating Endothelial Cell Migration, Proliferation, Cell Cycle Arrest, and Cell Senescence. Frontiers in Cardiovascular Medicine, 9, 851603.

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Quantifying Neuronal Activation and Protein Levels

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The areas of PVN and vlPAG from each slice were measured using Image-Pro Plus computer-assisted image analysis system (Media Cybernetics Inc., Bethesda, MD, USA) with to a light microscope (Olympus, Tokyo, Japan). The numbers of c-Fos-positive cells in the PVN and vlPAG were counted hemilaterally through a light microscope (Olympus). To compare the relative expressions of NF-200 and BDN, the detected bands were calculated densitometrically using Image-Pro Plus software (Media Cybernetics Inc.).
Statistical analysis was performed using one-way analysis of variance followed by Duncan post hoc test. The results are presented as the mean±standard error of the mean. Significance was set as P<0.05.

Shin K.M., Ko I.G., Kim S.E., Jin J.J., Hwang L., Kim S.H., Seo J.H., Kim B.K, & Na Y.G. (2018). Low-frequency electroacupncture improves locomotor function after sciatic crushed nerve injury in rats. Journal of Exercise Rehabilitation, 14(6), 927-933.

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