Loxl3

Manufactured by R&D Systems

LOXL3 is a protein that catalyzes the oxidative deamination of lysine residues in collagen and elastin. It is involved in the cross-linking of these extracellular matrix proteins. LOXL3 is expressed in various tissues, including the heart, skeletal muscle, and skin.

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Lab products found in correlation

Loxl2, by R&D Systems (1 mentions) Ros glo assay kit, by Promega (1 mentions) Loxl2, by Promega (1 mentions) Recombinant loxl2, by R&D Systems (1 mentions) Clear black plates, by Greiner (1 mentions)

2 protocols using loxl3

Extraction and Characterization of LOX Enzymes

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LOX enzyme
was extracted from pig skin by the method of Shackleton and Hulmes.^{51 (link)} LOXL2 and LOXL3 were purchased from R&D
Systems. LOX, LOXL2, and LOXL3 catalytic activity were determined
using the Promega ROS-Glo assay kit with cadaverine dihydrochloride
as substrate, BAPN as the reference inhibitor control, a preincubation
time of 20 min, 1 h, or 3 h, with nine dilutions from a top concentration
of 10 μM or 100 μM.

Smithen D.A., Leung L.M., Challinor M., Lawrence R., Tang H., Niculescu-Duvaz D., Pearce S.P., Mcleary R., Lopes F., Aljarah M., Brown M., Johnson L., Thomson G., Marais R, & Springer C. (2019). 2-Aminomethylene-5-sulfonylthiazole Inhibitors of Lysyl Oxidase (LOX) and LOXL2 Show Significant Efficacy in Delaying Tumor Growth. Journal of Medicinal Chemistry, 63(5), 2308-2324.

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Collagen Crosslinking Enzyme Quantification

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Black µclear plates (Greiner bio one, Austria) were coated with human lung collagen (EPC, Owensville, Missouri, USA) at 4 °C overnight. Plates were washed with PBS once followed by incubation with either 20 nM or 100 nM recombinant LOXL2 or LOXL3 (R&D Systems, Minneapolis, MN) for 2 hours. The collagen reaction was stopped using fixative and free aldehyde residues were fluorescently labeled using the Aldehyde Site Detection Kit (KA1295, Abnova, Germany). FITC fluorescence was measured using EnVision and the respective Software.

Aumiller V., Strobel B., Romeike M., Schuler M., Stierstorfer B.E, & Kreuz S. (2017). Comparative analysis of lysyl oxidase (like) family members in pulmonary fibrosis. Scientific Reports, 7, 149.

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