Lithium hydroxide monohydrate

Glycine (coded as GLY, 98%), L-alanine (ALA, 99%), L-valine (VAL, >99%), L-leucine (LEU, >98%), L-histidine (HIS, >99%), L-serine (SER, 99%), L-asparagine (ASN, >98%), L-glutamine (GLN, 99%), L-aspartic acid (ASP, >99%), L-glutamic acid monohydrate (GLU, >98%), N,N′-methylenebisacrylamide (MBA, 99%), lithium hydroxide monohydrate (98%), and HCl 1M (aqueous solution), were supplied by Sigma-Aldrich (Milano, Italy) and used as received.
Cotton (COT) with an area density of 240 gm⁻² was purchased from Fratelli Ballesio S.r.l. (Torino, Italy).

The following chemicals were used: the octanoic acid, butyric acid, p-toluenosulfonic acid monohydrate (p-TSA), lithium hydroxide monohydrate, fetal bovine serum (FBS) calcium hydroxide and diiodomethane (Sigma Aldrich, Poznań, Poland); phosphoric acid (POCH, Gliwice, Poland); the α-MEM modification essential medium, trypsin and Alamar blue (Thermofisher, Cambridge, UK); the fetal bovine serum, pencillin, streptomycin, Dulbecco’s phosphate-buffered saline (Dulbecco’s PBS), trypan blue, dimethyl sulfoxide, ethanol and phosphate buffer saline (Sigma Aldrich, Dorset, UK), the Dulbecco’s Modified Eagle Medium (DMEM) and 4′,6-diamidino-2-phenylindole (DAPI) (Thermofisher, Warsaw, Poland); the hydrochloric acid, methanol, ethyl acetate and chloroform (Chempur, Poland); the sodium chloride and the tetrahydrofuran chromatographic grade (Avantor Chemicals, Gdańsk, Poland); the methanol and acetonitrile HPLC grade (Chemsolve, Łódź, Poland), and the diclofenac acid (Fluorochem, Hadfield, UK).

For the magnetoelastic measurements, a commercial ribbon of Metglas 2826MB (Fe₄₀Ni₃₈Mo₄B₁₈) purchased from Hitachi Metals Europe GmbH (Dusseldorf, Germany) was used as resonant platform. Magnetoelastic sensors are made of this ribbon by cutting strips of 2 cm length after applying a cleaning treatment with analytical grade acetone purchased from Sigma-Aldrich Chemie GmbH (Taufkirchen, Germany).
For the ZnO nanoparticle synthesis and later film deposition the reagents used were analytical-grade without further purification. Lithium hydroxide monohydrate [Li(OH)·H₂O], zinc acetate dihydrate [Zn(CH₃COO)₂·H₂O] and ethanol were purchased from Sigma.
Hb (MW 65,000), from Bovine blood was purchased from Sigma and was used without further purification. Sodium dihydrogen orthophosphate (0.01 M) from Sigma was used to prepare the supporting electrolyte, and its pH was adjusted to 7 using NaOH and was thoroughly deaerated by bubbling with Argon prior to the experiments. H₂O₂ (30% w/v solution) was purchased from Lach-Ner (Neratovice, Czech Republic), and was diluted. All solutions were prepared with deionized water.

N,N′-methylenebisacrylamide (M, 99%), L-cystine (CYSS, 99%), lithium hydroxide monohydrate (LiOH∙H₂O, 98%), 1 M hydrochloric acid (HCl), cystamine dihydrochloride (CASS, >98%), triethylamine (TEA, >99%), diethyl ether, dichloromethane, and anhydrous sodium sulfate (Na₂SO₄) were supplied by Sigma-Aldrich (Milano, Italy). Diethyl vinylphosphonate (97%) was supplied by TCI Chemicals (Israel) and used as received. Cotton (COT) with an area density of 240 g∙m⁻² was purchased from Fratelli Ballesio S.r.l. (Torino, Italy).

The monoclonal antibody MAB-1-L001 (IgG₁), produced in CHO cells, was provided by the Manufacturing Technology Association of Biologics (Tokyo, Japan). Hydroxylamine solution (50 wt.% in water), lithium hydroxide monohydrate, and bovine apo-transferrin were purchased from Sigma-Aldrich (St. Louis, MO, USA). PNGase F and glycopeptidase A (from Almond) were purchased from Takara Bio Inc. (Otsu, Japan) and Seikagaku Biobusiness Corp. (Tokyo, Japan), respectively. 2-Picoline borane and 2-aminobenzamide were purchased from Junsei Chemical Co., Ltd. (Tokyo, Japan) and Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan), respectively. HRP and other chemicals and solvents were purchased from Wako Pure Chemical (Osaka, Japan).

After washing in 1× PBS, brains incubated in inactivation solution of 1% acrylamide (40% stock, Biorad #161–0140), 1 M glycine (Sigma-Aldrich, St. Louis, MO, #G7126) in 1× PBS. After washing in 1× PBS, brains were put into clearing solution of 200 mM sodium dodecyl sulfate (SDS) (Sigma-Aldrich, #L3771), 20 mM lithium hydroxide monohydrate (Sigma, #254274), 40 mM boric acid (Sigma-Aldrich, #7901), pH 8.5–9.0 and left shaking at 55 °C for 4 weeks until white matter tracts were translucent to the eye in SDS. Brains were washed in 1× PBS or Weak Binding Solution (WBS) for up to 1 week and immunolabeled with the SWITCH protocol for labeling intact mouse brain (Supplementary Methods).