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Red streptavidin fluorescent beads

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Red streptavidin-fluorescent beads are a type of lab equipment used for various applications. Streptavidin, a protein derived from the bacterium Streptomyces avidinii, is covalently coupled to fluorescent beads, producing a red-colored product. The core function of these beads is to enable the detection and capture of biotinylated molecules through the high-affinity interaction between streptavidin and biotin.

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Lab products found in correlation

Lsrii flow cytometer, by BD (2 mentions) Thp 1 cells, by Merck Group (1 mentions)

Close spelling variants of this product

Streptavidin beads (263 citations) Fluorescent beads (43 citations) Red fluorescent beads (9 citations) Fluorescent streptavidin (2 citations)

2 protocols using red streptavidin fluorescent beads

1

SARS-CoV-2 S Protein Binding Assay

Cited 1 time
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ADCP was measured as previously described77 (link). Briefly, biotinylated SARS-CoV-2 S stabilized trimer was incubated with red streptavidin-fluorescent beads (Molecular Probes) for 2 h at 37 °C. Ten μl of a 100-fold dilution of beads–protein mixture was incubated for 2 h at 37 °C with 100 μl of monoclonal antibodies diluted at 5 μg ml−1 before addition of THP-1 cells (20,000 cells per well; Millipore). After 19 h incubation at 37 °C, the cells were fixed with 2% formaldehyde solution and fluorescence was evaluated on a LSRII flow cytometer (BD Bioscience). The phagocytic score was calculated by multiplying the percentage of bead-positive cells by the geometric mean fluorescence intensity (MFI) of the bead-positive cells and dividing by 104.
Sankhala R.S., Lal K.G., Jensen J.L., Dussupt V., Mendez-Rivera L., Bai H., Wieczorek L., Mayer S.V., Zemil M., Wagner D.A., Townsley S.M., Hajduczki A., Chang W.C., Chen W.H., Donofrio G.C., Jian N., King H.A., Lorang C.G., Martinez E.J., Rees P.A., Peterson C.E., Schmidt F., Hart T.J., Duso D.K., Kummer L.W., Casey S.P., Williams J.K., Kannan S., Slike B.M., Smith L., Swafford I., Thomas P.V., Tran U., Currier J.R., Bolton D.L., Davidson E., Doranz B.J., Hatziioannou T., Bieniasz P.D., Paquin-Proulx D., Reiley W.W., Rolland M., Sullivan N.J., Vasan S., Collins N.D., Modjarrad K., Gromowski G.D., Polonis V.R., Michael N.L., Krebs S.J, & Joyce M.G. (2024). Diverse array of neutralizing antibodies elicited upon Spike Ferritin Nanoparticle vaccination in rhesus macaques. Nature Communications, 15, 200.
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2

SARS-CoV-1 and SARS-CoV-2 Antibody Phagocytic Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
ADCP was measured as previously described (75 (link)). Briefly, biotinylated SARS-CoV-1 or SARS-CoV-2 prefusion-stabilized S trimer was incubated with red streptavidin-fluorescent beads (Molecular Probes) for 2 h at 37 °C. Ten microliters of a 100-fold dilution of beads–protein was incubated for 2 h at 37 °C with 100 μL of 8,100-fold (SARS-CoV-2) or 900-fold (SARS-CoV-1) diluted plasma samples before addition of THP-1 cells (20,000 cells per well; Millipore Sigma). After 19 h incubation at 37 °C, the cells were fixed with 2% formaldehyde solution (Tousimis), and fluorescence was evaluated on an LSRII flow cytometer (BD Bioscience). The phagocytic score was calculated by multiplying the percentage of bead-positive cells by the geometric mean of the fluorescence intensity of bead-positive cells and dividing by 10,000.
King H.A., Joyce M.G., Lakhal-Naouar I., Ahmed A., Cincotta C.M., Subra C., Peachman K.K., Hack H.R., Chen R.E., Thomas P.V., Chen W.H., Sankhala R.S., Hajduczki A., Martinez E.J., Peterson C.E., Chang W.C., Choe M., Smith C., Headley J.A., Elyard H.A., Cook A., Anderson A., Wuertz K.M., Dong M., Swafford I., Case J.B., Currier J.R., Lal K.G., Amare M.F., Dussupt V., Molnar S., Daye S.P., Zeng X., Barkei E.K., Alfson K., Staples H.M., Carrion R., Krebs S.J., Paquin-Proulx D., Karasavvas N., Polonis V.R., Jagodzinski L.L., Vasan S., Scott P.T., Huang Y., Nair M.S., Ho D.D., de Val N., Diamond M.S., Lewis M.G., Rao M., Matyas G.R., Gromowski G.D., Peel S.A., Michael N.L., Modjarrad K, & Bolton D.L. (2021). Efficacy and breadth of adjuvanted SARS-CoV-2 receptor-binding domain nanoparticle vaccine in macaques. Proceedings of the National Academy of Sciences of the United States of America, 118(38), e2106433118.
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