In general, chemicals used in this study were commercially available and at least of analytical reagent grade. Sodium hydrogen phosphate dihydrate, sodium dihydrogen phosphate dihydrate, tris(2-carboxyethyl)phosphine (TCEP), HPLC-gradient grade acetonitrile (ACN), trichloroacetic acid (TCA), Cys-Gly, symmetrical disulfides of particular aminothiols, namely homocystine (Hcy2), cystine (Cys2) and oxidized GSH (Glu2) were from Sigma-Aldrich, (St. Louis, MO, USA). Hydrochloric acid, sodium hydroxide and perchloric acid (PCA) were from J.T. Baker (Deventer, The Netherlands). 2-Chloro-1-methylquinolinium tetrafluoroborate (CMQT)17 (link) and HPPTCA16 were prepared in our laboratory as previously described. Deionized water was produced in our laboratory. Commercially available 50 mg vitamin B6 tablets from Teva Pharmaceuticals (Cracow, Poland), containing active substance pyridoxine hydrochloride, were used.
Sodium dihydrogen phosphate dihydrate
Manufactured by Merck Group
Sourced in Germany, United States, Belgium, Italy
Sodium dihydrogen phosphate dihydrate is a chemical compound that is commonly used in laboratories. It is a white crystalline solid that is soluble in water. The compound is a source of phosphate ions and can be used in various applications, such as in buffer solutions, as a pH adjuster, and as a reagent in analytical procedures.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Merck Group (13 mentions)
Sodium hydroxide (naoh), by Merck Group (12 mentions)
Disodium hydrogen phosphate dihydrate, by Merck Group (10 mentions)
Acetic acid, by Merck Group (10 mentions)
Hydrochloric acid (hcl), by Merck Group (9 mentions)
Sulfuric acid, by Merck Group (7 mentions)
Disodium hydrogen phosphate, by Merck Group (7 mentions)
Disodium hydrogen phosphate dodecahydrate, by Merck Group (6 mentions)
Methanol, by Merck Group (5 mentions)
Methanol, by Thermo Fisher Scientific (5 mentions)
Acetonitrile, by Merck Group (5 mentions)
Phosphoric acid, by Merck Group (5 mentions)
Sodium hydroxide (naoh), by Avantor (4 mentions)
Potassium chloride (kcl), by Merck Group (4 mentions)
Bovine serum albumin (bsa), by Merck Group (4 mentions)
Sodium acetate, by Merck Group (4 mentions)
Hydrogen peroxide, by Merck Group (4 mentions)
Ethanol, by Merck Group (4 mentions)
Formic acid, by Merck Group (4 mentions)
Potassium persulfate, by Merck Group (4 mentions)
65 protocols using sodium dihydrogen phosphate dihydrate
1
Determination of Aminothiol Disulfides
2
Lipid Reagents for Membrane Studies
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Diphosphatidyl glycerol (cardiolipin, CL), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), cholesterol (Chol), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(carboxyfluorescein) (ammonium salt, DOPE-CF) (all from Avanti polar lipids, Alabaster, AL, USA) were used as received (the structures are presented in Table 3 ). Disodium hydrogen phosphate dihydrate (Na2HPO4⋅2H2O), sodium dihydrogen phosphate dihydrate (NaH2PO4⋅2H2O), and sodium chloride (all chemically pure grade and were purchased from Sigma-Aldrich, Darmstadt, Germany) were used as received. 10–3 M buffer solution was prepared by weighing.
Double-distilled water was used for making solutions after additional treatment with a Milli-Q (Merck Millipore, Darmstadt, Germany) system that included ion exchange adsorption columns for advanced purification from organic impurities and filters for removing large particles. The conductivity of the purified water was 0.5 μS/cm.
Double-distilled water was used for making solutions after additional treatment with a Milli-Q (Merck Millipore, Darmstadt, Germany) system that included ion exchange adsorption columns for advanced purification from organic impurities and filters for removing large particles. The conductivity of the purified water was 0.5 μS/cm.
3
Synthesis and Characterization of Gold Nanoparticles
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Sodium borohydride (NaBH4), [(1-methyl-1H-imidazol-2-yl)sulfanyl]acetic
acid (IL), and PVP were purchased from Sigma-Aldrich (St. Louis, Missouri,
USA). Tetrachloroauric(III) acid (HAuCl4, 99.99% pure-trace
metal basis, 30% wt in dilute HCl) was purchased from Sigma-Aldrich
(St. Louis, Missouri, USA). Disodium hydrogen phosphate dihydrate
(Na2HPO4·2H2O) and sodium dihydrogen
phosphate dihydrate (NaH2PO4·2H2O) were obtained from Sigma-Aldrich (St. Louis, Missouri, USA). AP
was kindly donated by ROKETSAN Corporation (i.e.,
an establishment of Turkish Armed Forces Foundation). The explosive
materials 2,4,6-trinitrotoluene (TNT), 1,3,5,7-tetranitro-1,3,5,7-tetraazacyclooctane
(HMX), 1,3,5-trinitro-1,3,5-triazacyclohexane (RDX), 2,4,6-trinitrophenyl-N-methylnitramine (tetryl), and pentaerythritoltetranitrate
(PETN) were kindly supplied by the Mechanical and Chemical Industry
Corporation (Makine Kimya Endustrisi Kurumu, MKEK) of Turkey from
previous projects. Picric acid was purchased from Sigma-Aldrich.
acid (IL), and PVP were purchased from Sigma-Aldrich (St. Louis, Missouri,
USA). Tetrachloroauric(III) acid (HAuCl4, 99.99% pure-trace
metal basis, 30% wt in dilute HCl) was purchased from Sigma-Aldrich
(St. Louis, Missouri, USA). Disodium hydrogen phosphate dihydrate
(Na2HPO4·2H2O) and sodium dihydrogen
phosphate dihydrate (NaH2PO4·2H2O) were obtained from Sigma-Aldrich (St. Louis, Missouri, USA). AP
was kindly donated by ROKETSAN Corporation (i.e.,
an establishment of Turkish Armed Forces Foundation). The explosive
materials 2,4,6-trinitrotoluene (TNT), 1,3,5,7-tetranitro-1,3,5,7-tetraazacyclooctane
(HMX), 1,3,5-trinitro-1,3,5-triazacyclohexane (RDX), 2,4,6-trinitrophenyl-N-methylnitramine (tetryl), and pentaerythritoltetranitrate
(PETN) were kindly supplied by the Mechanical and Chemical Industry
Corporation (Makine Kimya Endustrisi Kurumu, MKEK) of Turkey from
previous projects. Picric acid was purchased from Sigma-Aldrich.
4
Synthesis and Characterization of Ag3PO4 Semiconductors
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The silver nitrate (98%, Sigma-Aldrich) was used as a precursor for the synthesis of Ag3PO4 powder. PVP (Mw = 300,000), sodium dihydrogen phosphate dihydrate (NaH2PO4·2H2O, 99%), sodium phosphate decahydrate (Na3PO4·10H2O, 99%), N,N-dimethylformamide (DMF), hexamethylenetetramine (HMT), and urea (CO(NH2)2) were purchased from Sigma-Aldrich. Phosphoric acid (H3PO4, 85%), ammonia (NH3·H2O, 10%), and ethanol (CH3CH2OH, 96%) were purchased from POCH S.A., Poland. All chemicals were used without further purification. The morphology of Ag3PO4 semiconductors was measured by scanning electron microscope (SEM, JEOL JSM-7610F) working in high vacuum mode. DRS UV–Vis spectra of the synthesized samples were recorded in the scan range 300–700 nm using UV–Vis spectrophotometer (Evolution 220, Thermo Scientific) and BaSO4 as the reference.
5
HPLC Analysis of GABA in Microdialysis
6
Microdialysis Experiments with GABA and Nipecotic Acid
7
Gemini Surfactants for Protein Studies
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Gemini surfactants 3,3′-[1,8-(2,7-dioxaoctane)]bis(1-dodecylimidazolium) chloride (oxyC4) and 3,3′-[1,12-(2,11-dioxadodecane)]bis(1-dodecylimidazolium) chloride (oxyC8) were synthesized in a modified way described earlier [32 (link), 33 (link)]. Their chemical structure is presented in Fig. 1 . BSA, HEWL and sodium dihydrogen phosphate dihydrate were purchased from Sigma Aldrich.
All samples were prepared as protein-surfactant solutions with use of phosphate buffer solution at pH equal to 7.3 ± 0.4.
All samples were prepared as protein-surfactant solutions with use of phosphate buffer solution at pH equal to 7.3 ± 0.4.
8
Analytical Characterization of Pharmaceutical Formulations
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In the present work, all reagents used were of an analytical standard and were used without pre-processing. CFT was supplied by Sigma-Aldrich. Spectracef® pediatric sachets, the pharmaceutical dosage form, were obtained from a topical pharmacy. Drug-free human serum from male AB plasma was received from Sigma-Aldrich. Sodium acetate trihydrate (>99%), acetic acid (>99%), phosphoric acid (>85%), acetonitrile (99.8%), methanol (99.8%), sodium dihydrogen phosphate dihydrate (>99%), sodium phosphate monobasic (≥99.0%), sodium phosphate (≥99.0%), sulfuric acid (95–97%), sodium hydroxide (>97%), N, N-dimethyl formamide (DMF), boric acid (>99%), hydrochloric acid (37%), potassium chloride (KCl), magnesium sulphate (MgSO4), sodium nitrate (NaNO3), ascorbic acid, glucose, dopamine, uric acid, and paracetamol were procured from Sigma-Aldrich. Fe2O3, chitosan NCs, and the MWCNTs were obtained from Sigma-Aldrich.
9
Electrochemical Analysis of Axitinib Drug
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AXI and its tablet dosage form
Inlyta were provided AXI and its tablet dosage form Inlyta was provided
by DEVA Holding A.S. (Istanbul, Turkey). Acetic acid (≥99%),
boric acid (≥99.5%), methanol (99.8%), phosphoric acid (>85%),
sodium acetate trihydrate (>99%), sodium dihydrogen phosphate dihydrate
(>99%), sodium hydroxide (>97%), sodium phosphate (96%), sodium
phosphate
monobasic (≥99%), sulfuric acid (95–97%), and drug-free
human serum were obtained from Sigma-Aldrich. MWCNTs (>90% carbon
basis, D × L 110–170
nm × 5–9 μm), Fe2O3 NPs (nanopowder,
<50 nm particle size), and chitosan were also supplied from Sigma-Aldrich.
A 1 × 10–3 M AXI standard stock solution
was prepared in methanol and kept in a refrigerator at 4 °C.
Working solutions containing 20% methanol were prepared by dilution
from the AXI stock solution with the supporting electrolyte solution.
Buffer solutions of sulfuric acid solutions (pH 0.3–1.0), phosphate
buffer solutions (pH 1.5–8.0), Britton–Robinson (BR)
buffer solutions (pH 2.0–8.0), and acetate buffer solutions
(pH 3.7–5.7) were prepared in double-distilled water and kept
in a refrigerator at 4 °C.
Inlyta were provided AXI and its tablet dosage form Inlyta was provided
by DEVA Holding A.S. (Istanbul, Turkey). Acetic acid (≥99%),
boric acid (≥99.5%), methanol (99.8%), phosphoric acid (>85%),
sodium acetate trihydrate (>99%), sodium dihydrogen phosphate dihydrate
(>99%), sodium hydroxide (>97%), sodium phosphate (96%), sodium
phosphate
monobasic (≥99%), sulfuric acid (95–97%), and drug-free
human serum were obtained from Sigma-Aldrich. MWCNTs (>90% carbon
basis, D × L 110–170
nm × 5–9 μm), Fe2O3 NPs (nanopowder,
<50 nm particle size), and chitosan were also supplied from Sigma-Aldrich.
A 1 × 10–3 M AXI standard stock solution
was prepared in methanol and kept in a refrigerator at 4 °C.
Working solutions containing 20% methanol were prepared by dilution
from the AXI stock solution with the supporting electrolyte solution.
Buffer solutions of sulfuric acid solutions (pH 0.3–1.0), phosphate
buffer solutions (pH 1.5–8.0), Britton–Robinson (BR)
buffer solutions (pH 2.0–8.0), and acetate buffer solutions
(pH 3.7–5.7) were prepared in double-distilled water and kept
in a refrigerator at 4 °C.
10
Bovine Gelatin-Based Composite Materials
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Gelatin from bovine skin (75 Bloom), sodium chlorite, sodium acetate, acetic acid, glycine, sodium dihydrogen phosphate dihydrate and sodium phosphate dibasic dihydrate were purchased from Sigma-Aldrich and used as received. Genipin powder (98%, HPLC) was a product of Zhixin Biotechnology, China. Wood blocks (10 × 10 × 10 mm3, Longitudinal × Radial × Tangential) were cut from air-dried balsa wood (Ochroma pyramidale) sticks with a density of 175 kg/m3 purchased from Wentzel’s Co. Ltd., Sweden.
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