Recombinant integrin αvβ3

Antibodies against integrins (αv, α5, α6, β1, β3, β4, and β5), the apoptosis-related protein cleaved caspase-8, and the autophagy marker LC3 were all purchased from Cell Signaling Technology (CST, Tokyo, Japan). Alexa-conjugated anti-mouse and anti-rabbit IgG were purchased from Life Technologies (Eugene, OR, USA). Recombinant integrin αvβ3 and B7-H4 were purchased from R&D Systems (Minneapolis, MN, USA). B7-H4 siRNA and non-targeting control siRNA were purchased from Dharmacon (Lafayette, CO, USA).

Human vitronectin was obtained from BD (Bedford, MA). The neutralizing antibodies against integrin αvβ3 (LM609) and αvβ5 (P1F6) were obtained from Millipore Corporation (Bedford, MA) and the antibody against integrin β1 (4B7) was obtained from Calbiochem (San Diego, CA). Biotinylated-GRGDS (GRGDS, LC-biotin labeled) and Cyclo-(GRGDSP) were purchased from AnaSpec (San Jose, CA). Fifteen kinds of customized peptides were synthesized and>95% purity was examined by Operon Biotechnologies Japan (Tokyo, Japan) or PH Japan (Hiroshima, Japan). Recombinant integrin αvβ3 and αvβ5 were purchased from R&D Systems (Minneapolis MN). The rabbit polyclonal antibody against integrin αv (C2C3) was purchased from GeneTex (Irvine, CA).