Silicone oil with a viscosity of 10,000 cSt, rhodamine fluorescent dye (Rhodamine 6G) and phosphate-buffered saline (PBS) were all purchased from Sigma-Aldrich (St. Louis, MO, USA). The silicone elastomer kit for fabricating the polydimethylsiloxane (PDMS) chip was purchased from Dow Corning (Sylgard 184, Auburn, MI, USA). The specific substrate (DDAO-ol) was synthesized in our laboratory. Bile salt was obtained from the Dalian Meilun Biotech Co., Ltd. (Dalian, China). Tris was purchased from Roche Diagnostics GmbH (Mannheim, Germany). Tris-HCl buffer (pH 7.4, 25 mM, 150 mM NaCl, 1 mM CaCl2) was prepared by using Milli-Q Water (Millipore, Danvers, MA, USA). Orlistat was purchased from the Dalian Meilun Biotech Co., Ltd. (Dalian China). 1,2,3,4,6-penta-O-galloyl-β-D-glucopyranose (PGG) was provided from Jiangyin Tianjiang Pharmaceutical Co., Ltd. (Jiangyin, China). Sciadopitysin was ordered from Shanghai Standard Biotech Co., Ltd. (Shanghai, China). Dimethyl sulfoxide (DMSO) was purchased from Thermo Fisher Scientific (Fair Lawn, NJ, USA).
Orlistat
Manufactured by Meilun
Sourced in China
Orlistat is a laboratory equipment product that functions as a lipase inhibitor. It is designed to prevent the breakdown and absorption of dietary fats within the human body.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (2 mentions)
Milli q water, by Merck Group (1 mentions)
Silicone oil, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Rhodamine 6g, by Merck Group (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
4 methylumbelliferone 4 mu, by Merck Group (1 mentions)
2 protocols using orlistat
1
Fluorescent Silicone Oil Flow Assay
2
Inhibition of Human Pancreatic Lipase
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Seventy-three herbal extracts were provided by Jiangyin Tianjiang Pharmaceutical Co., Ltd. (Jiangyin, China). Further, 17 natural constituents identified from liquid chromatography (LC) fractions of ERC, namely, 1) epicatechin, 2) p-coumaric acid, 3) ferulic acid, 4) epicatechin gallate (ECG), 5) catechin gallate (CG), 6) 1,2,3,4,6-penta-O-galloyl-β-d -glucopyranose (PGG), 7) luteolin-7-O-β-d -glucoside, 8) afzelin, 9) quercetin-3-O-glucoside, 10) rhodiosin, 11) quercetin-3-O-glucuronide, 12) rhodionin, 13) quercitrin, 14) eriodictyol, 15) quercetin, 16) luteolin, and 17) kaempferol, were obtained from Shanghai Standard Technology Co., Ltd. (Shanghai, China), and the purities were higher than 96%. The positive hPL inhibitor (orlistat, a commercial hPL inhibitor) was purchased from Dalian Meilun Biotech Co., Ltd. (Dalian, China). hPL was expressed and purified as described previously [30 ]. 4-methylumbelliferone (4-MU) was obtained from Sigma-Aldrich (Shanghai) Trading Co., Ltd. (Shanghai, China), and 4-methylumbelliferyl oleate (4-MUO) was purchased from J&K Scientific Co., Ltd. (Beijing, China). Liquid chromatography-mass spectrometry (LC-MS) grade formic acid, acetonitrile, methanol, and dimethyl sulfoxide (DMSO) were purchased from Thermo Fisher Scientific (Fair Lawn, NJ, USA).
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