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18 protocols using sodium dodecyl sulfonate

1

Aptamer-based Nanomaterial Synthesis

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The aptamer sequence was selected according to the relevant reports of Teh et al. [38 (link)].
Aptamer 1, 5′ -P-ACT CAC TAT/ra/GGA AGAGAT GAA AAA AAA AA-NH2-3′.
Aptamer 2, 5′-CATCTCTTCTCCGAGCCGGTCGAA ATA GTG AGT-P-3.′
The design mentioned above of the accounting adaptation sequence was synthesized by Bioengineering (Shanghai, China) Co., Ltd., purified by HPLC and verified by mass spectrometry. Sodium dodecyl sulfonate (SDS) was ordered to Sigma, polystyrene (P.S.) nanoparticles were ordered to Suzhou Derivative Biotechnology Co., Ltd. (Suzhou, China), dichloromethane was ordered to Aladdin Reagent (Shanghai, China) Co., Ltd., tetrastyrene monoquaternary ammonium salt (DSAI) was purchased from Xi’an Qiyue Biotechnology Co., Ltd. (Xi’an, China), polyethylene glycol (PEG) was purchased from Beijing Bailingwei Biotechnology Co., Ltd. (Beijing, China) A 0.22 μm water filter was purchased from Changde Bickerman Biotechnology Co., Ltd. (Changde, China), and Zr-MOFs/PCN-222 was purchased from Xi’an Qiyue Biotechnology Co., Ltd. (Xi’an, China).
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2

Analytical Technique for Soil Contaminants

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The used substrate papers, glass fiber film (SB08, CB06, BT53, RB45), polyester fiber film (VL78), and special absorbent paper (SX18) were purchased from Shanghai Jinbiao Biological Technology Co., Ltd. (Shanghai, China), cadmium oxide, selenium powder, liquid paraffin, oleic acid, oleyl-amine, tetra-methoxy-silane(TMOS), tetra-ethoxy-silane (TEOS), tetra-propoxy-silane (TPOS), 3-aminopropyltriethoxylsilane (APTES), trimethoxy-silyl-propane-thiol (MPTMS), and vinyl-trimethoxy-silane(VMS) were supplied by Aladdin (Shanghai, China); urea, sodium dodecyl sulfonate (SDS), sodium chloride, trichlorfon, acephate, fenthion and dimethoate were supplied by Sigma-Aldrich (Shanghai, China). Soybean samples were purchased from a local market in Xi’an (Shaanxi, China). All the water used in the experiment was ultrapure. All reagents used were of at least analytical grade.
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3

Aptamer-based Biosensor for Pesticide Detection

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Chloroauric acid tetrahydrate (HAuCl4·4H2O), trisodium citrate, sodium citrate dihydrate, Triton X-100, mycose, sodium dodecyl sulfonate (SDS), NaCl, Na3PO4·12H2O, Tween-20, sucrose, deoxyadenosine triphosphate (dATP), bovine serum albumin (BSA), phosphate buffered saline (PBS, pH 7.4, 0.01 M), sodium chloride-sodium citrate (SSC) buffer (20 concentrate, pH 7.0), borate buffer (BB, pH 9.0, 0.1 M), and the pesticides used in this study (chlopyrifos, malathion, diazinon, atrazine, carbaryl, acetamiprid, and 2,4-D.) were purchased from Sigma Chemical Company (St. Louis, MO, USA). Streptavidin was purchased from Invitrogen (Carlsbad, CA, USA). QDs nanobeads premodified by polystyrene maleic-anhydride copolymer were provided by Shanghai Kundao Biotech Co., Ltd. (Shanghai, China). Backing cards (HF000MC100), glass fiber sample pads (CFSP001700), conjugation pads (GFCP000800), nitrocellulose membranes (135s), and absorbent pads (CFSP001700) were purchased from Millipore (Bedford, MA, USA). Sequences of aptamers for chlorpyrifos (Jiao et al., 2017 , 2016 ), aptamers for diazinon (Jokar et al., 2017 (link)), aptamers for malathion (Bala et al., 2016 (link)) and corresponding biotinylated complementary sequences (Table S1), were synthesized and purified by Sangon Biotech Co., Ltd. (Shanghai, China). Double distilled water (ddwater) was used in all experiments.
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4

Rhein-based Cytotoxicity Assay Protocol

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Rhein was obtained by Xi’an Natural Field Bio-Technique Co., Ltd. Sodium bicarbonate NaHCO3 was provided from Sinopharm Chemical Reagent Co., Ltd. Phosphate buffer solutions (PBS) were purchased from Xiya Reagent (Chengdu, China). MTT reagent, dimethylsulfoxide (DMSO), destination access point identifier (DAPI), polyacrylamide and sodium dodecyl sulfonate were purchased from Sigma. Ultrapure water (>18 MΩ) was used. All the reagents were used without further purification throughout this study.
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5

Egg-Based Biochemical Assay Protocol

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Eggs were purchased from a supermarket. Sodium dodecyl sulfonate (SDS), 1-phenylaminonaphthalene-8-sulfonic acid (ANS) and 5,5 ‘dithiobis (2-nitrobenzoic acid) (DTNB) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Other chemicals were of analytical grade.
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6

Fluorescent Dextran Polyelectrolyte Layers

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Polyelectrolytes polystyrenesulfonate sodium (PSS) and polyallylamine hydrochloride (PAH) with a molecular mass of 70 kDa, fluorescein isothiocyanate–dextran (150 kDa) Sigma (USA), Ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA) purchased in Sigma (St. Louis, MS, USA), sodium dodecyl sulfonate (SDS), sodium carbonate, calcium chloride from “Reahim”.
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7

Aptamer-Functionalized Magnetic Nanomaterials

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Aptamer 1, 5′-GTG ACG CTC CTA ACG CTG ACT CAG GTG CAG TTC TCG ACT CGG TCT TGA TGT GGG TCC TGT CCG TCC GAA CCA ATC-SH-3′.
Aptamer 2, 5′-NH2-GTG ACG CTC CTA ACG CTG ACT CAG GTG CAG TTC TCG ACT CGG TCT TGA TGT GGG TCC TGT CCG TCC GAA CCA ATC-3′.
3-mercaptopropyltrimethoxysilane (MPTMS) and sodium dodecyl sulfonate (SDS) were ordered from Sigma, Tokyo, Japan. Fe3O4 nanomaterials and polystyrene (PS) nanoparticles were ordered from the Suzhou derivative Biotechnology Co., Ltd., Suzhou, China, whereas dichloromethane was ordered from the Aladdin Reagent Co., Ltd., Shanghai, China.
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8

Proteomic Analysis Using Ionic Liquids

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The ionic liquids, 1-butyl-3-methylimidazolium thiocyanate (C4Im-SCN) and 1-dodecyl-3-methylimidazolium chloride (C12Im-Cl), were obtained from Shanghai Cheng Jie Chemical (Shanghai, China). Sodium dodecyl sulfonate (SDS), bovine serum albumin (BSA), tris(hydroxymethyl)aminomethane (Tris), tris (2-carboxyethyl) phosphine (TCEP), iodoacetamide (IAA), ethylene diamine tetraacetic acid (EDTA), ethylene glycol tetraacetic acid (EGTA), ammonium bicarbonate (NH4HCO3) and protease inhibitor mixture were purchased from Sigma-Aldrich. Urea was bought from Fluka (Buchs, Germany), and sequencing-grade modified trypsin was from Promega. Acetonitrile (ACN) and methanol were purchased from Merck (Darmstadt, Germany). The Microcon filtration device with a relative molecular mass cut-off of 10 kDa was from Sartorius AG (Goettingen, Germany). Deionized water was purified using a Milli-Q system from Millipore. Other chemicals were of analytical grade. Durashell C18 particles (5 μm, 150 Å pore) were obtained from Bona-Agela Technologies (Tianjin, China). Reprosil-Pur C18-AQ particles (3 μm, 120 Å pore) were obtained from Dr. Maisch GmbH (Ammerbuch-Entringen, Germany). Fused-silica capillaries (100 μm i.d./360 μm o.d.) were purchased from Sino Sumtech (Handan, Hebei, China).
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9

Nanoparticle Synthesis and Purification

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N-methyl pyrrolidone (NMP), dichloromethane (DCM), tetrahydrofuran (THF), hydrochloric acid, toluene, ethanol, and potassium carbonate (K2CO3) were purchased from Chengdu Chron Chemicals. Phenolphthalein (PPL), 2, 6-difluorobenzonitrile (DFBN), and sodium dodecyl sulfonate (SDS) were received from Sigma Aldrich. Core-shell quantum dots (QD, ZnCdSe@ZnS) were obtained from Wuhan Jiayuan Quantum Dots Co., Ltd. Agarose beads (AG Beads), protein A, protease inhibitor, RIPA lysate buffer, PBS powder, and SDS-PAGE 2× loading buffer were purchased from Sangon Biotech (Shanghai) Co., Ltd. All chemicals were used as obtained without further treatment.
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10

Oxidative Stress Evaluation in Cell Cultures

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Vitamin C, dimethylsulfoxide (DMSO), sodium dodecyl sulfonate (SDS), naphthylethylenediamine dihydrochloride, sulphanilamide, phosphoric acid (H3PO4), ethylene diamine tetraacetic Acid (EDTA), 2′,7′-dichlorofluorescein diacetate (DCFH-DA), 3-4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazoliumbromide (MTT), o-Phthalaldehyde (OPA), and n-ethylmaleimide (NEM) were obtained from Sigma, USA. High glucose DMEM medium, penicillin sodium and streptomycin were obtained from Gibco, USA. Fetal bovine serum (FBS) were purchased from PAN, Germany. Commercial kits for the analysis of superoxide dismutase (SOD), xanthine oxydase (XOD) and myeloperoxidase (MPO) were purchased from Nanjing Jiancheng Bioengineering Institute, China. All other reagents were analytical grade and used as received.
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