Sc 376248

Manufactured by Abcam

Sc-376248 is a primary antibody product offered by Abcam. It is a rabbit polyclonal antibody that recognizes a specific target antigen. The core function of this product is to serve as a research tool for detecting and studying the target protein in various applications.

Automatically generated - may contain errors

Lab products found in correlation

H3k9me2, by Merck Group (1 mentions) Complete protease inhibitor cocktail, by Roche (1 mentions) H3k27me3, by Cell Signaling Technology (1 mentions) Imager 600, by Cytiva (1 mentions) Ab1791, by Merck Group (1 mentions) T5168, by Merck Group (1 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Ab8898, by Abcam (1 mentions) Hpa020028, by Merck Group (1 mentions) Ab16048, by Abcam (1 mentions) Amersham imager, by Cytiva (1 mentions)

2 protocols using sc 376248

Chromatin Profiling of Mammalian Cells

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Whole-cell extracts of ~0.5x10⁶ cells were prepared by washing cultures in PBS and lysing with 50 μL lysis buffer (Tris pH 7.6, 10% SDS, Roche cOmplete Protease Inhibitor Cocktail). Western blotting was performed according to standard procedures using the following antibodies and dilutions: H3K27me3 (1:1000 Cell Signaling C36B11, rabbit), H3K9me2 (1:1000 Millipore 07-441, rabbit), LMNA (1:800 Santa Cruz Biotechnology sc-376248, mouse), LBR (1:1000, Abcam, ab122919, rabbit).

Schep R., Brinkman E.K., Leemans C., Vergara X., van der Weide R.H., Morris B., van Schaik T., Manzo S.G., Peric-Hupkes D., van den Berg J., Beijersbergen R.L., Medema R.H, & van Steensel B. (2021). Impact of chromatin context on Cas9-induced DNA double-strand break repair pathway balance. Molecular Cell, 81(10), 2216-2230.e10.

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Western Blot Analysis of Nuclear Proteins

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Cells were collected and lysed in RIPA buffer supplemented with protease inhibitor cocktail (Millipore, 535142). Protein concentration was quantified using the Pierce BCA protein assay kit (Pierce Biotechnology, 23225). After separation via SDS–PAGE, proteins were transferred to nitrocellulose membranes, blocked in 5% skimmed milk/PBST, and incubated with appropriate primary antibodies: lamin A/C (Abcam, ab8984; 1:1000), lamin A/C (Santa Cruz, sc-376248; 1:1000), lamin B1 (Abcam, ab16048; 1:1000), H3k9me3 (Abcam, ab8898; 1:5000), H3 (Abcam, ab1791; 1:5000), Ryr2 (Sigma, HPA020028, 1:1000), α-tubulin (Sigma, T5168, 1:1000) followed by incubation with a corresponding secondary antibody. The images were acquired on Amersham Imager 600.

Wang Y., Elsherbiny A., Kessler L., Cordero J., Shi H., Serke H., Lityagina O., Trogisch F.A., Mohammadi M.M., El-Battrawy I., Backs J., Wieland T., Heineke J, & Dobreva G. (2022). Lamin A/C-dependent chromatin architecture safeguards naïve pluripotency to prevent aberrant cardiovascular cell fate and function. Nature Communications, 13, 6663.

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