Dulbecco’s Modified Eagle Medium (DMEM), heat-inactivated low-endotoxin fetal bovine serum (FBS), and gentamicin purchased from Cambrex (Walkersville MD, USA) for tissue culture. All other cancer cell lines were purchased from American Type Culture Collection (Manassas, VA, USA). Most of these following compounds (thiostrepton, ampicillin, dexamethasone, 2-methoxyestradiol, (−) riboflavin, ascorbic acid, amiloride-HCL, (−) Corey lactone, and quinine sulphate) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA), and quercetin was purchased from Alfa Aesar (Johnson Matthey Co. Lancastor, UK). The 50% purified δ-tocotrienol fraction from annatto seeds was purchased from American River (Boston, MA, USA). RNeasy mini kit was purchased from QIAGEN Sciences (Germantown, MD, USA). DeltaGold (125 mg soft gels) from annatto seeds (composition 90% δ-tocotrienol + 10% γ-tocotrienol) was obtained as a gift by American River Nutrition, Inc. (Hadley, MA. USA). The hepatitis C antibody test kit was purchased from Sigma Chemical Co., St. Louis, USA. The second kit for diagnosing hepatitis C test is RNA (PCR) test. Pure total mRNA was obtained from the EDTA treated fresh whole blood using total RNA purification kit # 17200 (NORGEN Bioteck Corporation, Thorold, ON, Canada).
2 methoxyestradiol
Manufactured by Merck Group
Sourced in United States
2-methoxyestradiol is a laboratory reagent that is commonly used as a research tool in cell biology and biochemistry studies. It is a naturally occurring estrogen metabolite that has been found to have various biological activities, including potential anti-tumor and anti-angiogenic properties. The core function of 2-methoxyestradiol is to serve as a research compound for investigating cellular processes and signaling pathways.
Automatically generated - may contain errors
Lab products found in correlation
Halt protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (3 mentions)
Dylight 800 conjugated goat anti rabbit igg antibody h l, by Thermo Fisher Scientific (3 mentions)
Tov112d, by American Type Culture Collection (2 mentions)
Hyclone dmem culture media with and without phenol red, by Thermo Fisher Scientific (2 mentions)
Dylight 680 conjugated goat anti rabbit igg antibody h l, by Thermo Fisher Scientific (2 mentions)
Alexa 594 donkey anti rabbit secondary, by Jackson ImmunoResearch (2 mentions)
Sb203580 p38 mapk inhibitor, by Cayman Chemical (2 mentions)
2 hydroxyestradiol, by Merck Group (2 mentions)
Rneasy mini kit, by Qiagen (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Ampicillin, by Merck Group (1 mentions)
Quercetin, by Thermo Fisher Scientific (1 mentions)
Thiostrepton, by Merck Group (1 mentions)
Riboflavin, by Merck Group (1 mentions)
Gentamicin, by Cambrex (1 mentions)
Quinine sulphate, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Heat inactivated low endotoxin fetal bovine serum, by Cambrex (1 mentions)
Amiloride hcl, by Merck Group (1 mentions)
Alexa fluor 488 conjugate, by Thermo Fisher Scientific (1 mentions)
15 protocols using 2 methoxyestradiol
1
Comprehensive Methods for Cell Culture
2
Quantitative Fluorescent Protein Assay
3
Evaluating PKCδ Signaling in Cell Lines
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BG1 cells were obtained from the laboratory of Dr. Ken Korach at NIEHS and Hey C2 cell line was obtained from Dr. Jean Hurteau at Northshore University Health, Evanston Hospital. TOV112D (CRL11731) cell line was purchased from ATCC. HyClone DMEM culture media (with and without phenol red) from ThermoFisher (SH30604.02); 2-methoxyestradiol from Sigma-Aldrich (M6383); 100× HALT protease and phosphatase inhibitor cocktail from ThermoFisher (78440); DyLight™800 conjugated goat anti-rabbit IgG antibody (H&L) (35571) and DyLight™680 conjugated goat anti-rabbit IgG antibody (H&L) (35518) from Thermofisher. Alexa-594 donkey anti-rabbit secondary (133200) from Jackson Immuno Research. jet-PEI® purchased from Polyplus transfection (catalog no.101-10). Expression plasmids encoding PKCδWT (Addgene plasmid # 16386), PKCδDN (Addgene plasmid # 16389), and PKCδCAT (Addgene plasmid # 16388) were gifts from Bernard Weinstein [74 (link)], pSUPER-PKCδRNAi (Addgene plasmid # 10819) was a gift from Alex Toker [75 (link)] and pSUPER-scramble (Addgene plasmid # 118349) was a gift from Lea Sistonen [76 (link)]. E. Z. N. A.® plasmid maxi kit (D6922-02) was purchased from Omega Bio-Tek. SB203580 p38 MAPK inhibitor was purchased from Cayman Chemical (13067).
4
Glucocorticoid Receptor Regulation of miRNA-210
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M199 medium was obtained from Hyclone (Logan, UT); MiRNA-210 mimic, HiPerFect transfection reagent, miScript II RT kit, miScript Primer Assay and miScript SYBR Green PCR kit were purchased from Qiagen (Valencia, CA). MiR-210 locked nucleic acid (miR-210-LNA) were purchased from Exiqon (Woburn, MA). RU486, 2-methoxyestradiol, bromodeoxyuridine and NXTRACT Cell Lytic Nuclear Extraction Kit were purchased from Sigma-Aldrich (St. Louis, MO). TRIzol reagent, SuperScript III First-Strand Synthesis SuperMix and Annexin-V-FITC apoptosis kit were obtained from Life Technologies (Carlsbad, CA); The Dual-Glo Luciferase Assay System and pmiRGLO luciferase vector were purchased from Promega (Fitchburg, WI). GR siRNAs were purchased form Dharmacon (Lafayette, CO). The iQ SYBR Green Supermix were purchased from Bio-Rad (Hercules, CA). X-treme GENE HP DNA transfection reagent were purchased from Roche (Indianapolis, IN). RIPA lysis buffer and HRP-conjugated secondary antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). BCA protein assay kit and enhanced chemiluminescence reagents were purchased from Pierce (Rockford, IL). Anti-GR primary antibody were purchased from Cell Signaling (Danvers, MA) and anti-GAPDH primary antibody from Abcam (Cambridge, MA).
5
Estrogen Metabolism Quantification
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Estrone(E1), 17β-estradiol(E2), estriol(E3), 2-hydroxyestrone(2-OHE1), 2-hydroxyestradiol(2-OHE2), 2-methoxyestrone(2-MeOE1), 2-methoxyestradiol(2-MeOE2), 4-hydroxyestrone(4-OHE1), 4-hydroxyestradiol(4-OHE2), 4-methoxyestrone(4-MeOE1), 4-methoxyestradiol(4-MeOE2), 16α-hydroxyestrone(16α-OHE1), d5-E2, dansyl chloride, Vitamin C, naloxone, naloxone-glucuronide, D-Saccharic acid 1,4-lactone monohydrate and UGPGA were supplied by Sigma-Aldrich (St. Louis, USA) and Steraloids (Rhode, USA).
Tamoxifen was obtained from Aladdin (Shanghai, China). Alamethicin was obtained from J&K Scientific. TRIzol® and PrimeScript RT Reagent Kit were purchased from TaKaRa Bio-technology Co., Ltd. (Beijing, China). Human liver microsomes (HLMs) were acquired from Rild Liver Disease Research Co., Ltd (Shanghai). All analytical solvents were of liquid chromatography (LC) - grade and were obtained from Sigma-Aldrich (St. Louis, USA) or Merck (Darmstadt, Germany).
Tamoxifen was obtained from Aladdin (Shanghai, China). Alamethicin was obtained from J&K Scientific. TRIzol® and PrimeScript RT Reagent Kit were purchased from TaKaRa Bio-technology Co., Ltd. (Beijing, China). Human liver microsomes (HLMs) were acquired from Rild Liver Disease Research Co., Ltd (Shanghai). All analytical solvents were of liquid chromatography (LC) - grade and were obtained from Sigma-Aldrich (St. Louis, USA) or Merck (Darmstadt, Germany).
6
Quercetin and 2-Methoxyestradiol Formulation
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Quercetin, 2-Methoxyestradiol, hydroxypropyl-β-cyclodextrin (HPβCD) and carboxymethyl cellulose (CMC) were purchased from Sigma (St. Louis, MO, USA). RPMI-1640 medium, 0.25% trypsin-EDTA and fetal bovine serum were got from Hyclone (Logan, UT, USA), and Matrigel was from BD Biosciences (Franklin Lakes, NJ, USA).
7
A549 Cell Line Pharmacological Assay
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A549 cell line (ATCC® CCL-185™) was kindly offered by the University of Rzeszów, Poland. 2-methoxyestradiol, 2-hydroxyestradiol, supplements and cell culture media were obtained from Sigma Aldrich. Palmostatin B was purchased from Merck Millipore (178501).
The laboratory equipment used at the Department of Medical Chemistry, Medical University of Gdańsk, in order to process blood samples from patients and the control group was bought from Santa Cruz Biotechnology, Inc.
The laboratory equipment used at the Department of Medical Chemistry, Medical University of Gdańsk, in order to process blood samples from patients and the control group was bought from Santa Cruz Biotechnology, Inc.
8
Anticancer Activity of 2-Methoxyestradiol
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2-methoxy-estradiol (> 99% purity), and ALA were procured from Sigma, USA. HPLC grade acetonitrile and methanol were procured from Sigma, USA. Trizol was purchased from Invitrogen, Paisley, UK. The rest of the chemicals used in this project were of analytical grade. The human tumor cell line MCF-7 used in this study was obtained from the VACSERA (Giza, Egypt) cell culture unit that were originally acquired from ATCC (Manassas, VA, USA).
9
Comparative Analysis of Cell Line Responses
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The HeyC2 cell line [62 (link)] was obtained from Dr. Jean Hurteau at Northshore University Health-Evanston Hospital; TOV112D (CRL11731) cell line was purchased from ATCC. BG1 cells were obtained from Dr. Ken Korach’s lab at NIEHS [63 (link), 64 (link)]. HyClone DMEM culture media (with and without phenol red) from ThermoFisher (SH30604.02); 2-methoxyestradiol from Sigma-Aldrich (M6383); SB203580 p38-MAPK inhibitor from Cayman chemical (13067); DHA (3687) from Tocris Biosciences; 100x HALT protease and phosphatase inhibitor cocktail from ThermoFisher (78440); DyLight™800 conjugated goat anti-rabbit IgG antibody (H&L) (35571) and DyLight™680 conjugated goat anti-rabbit IgG antibody (H&L) (35518) from Thermofisher. Alexa-594 donkey anti-rabbit secondary (133200) from Jackson Immuno Research; DeadEnd Fluorometric TUNEL system kit (G3250) and CellTitre 96® Aqueous one solution cell proliferation assay kit (G3582) were both purchased from Promega (Madison, WI, USA).
10
Regenerative effects of small molecules
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