Atherosclerotic lesions in the aortic root were measured as previously reported [29 (link),30 (link)]. Briefly, at the end of the experiments, the vasculature of mice was perfused with 4% paraformaldehyde for approximately 5 min through the heart. The aortic root and adjacent heart were excised and sectioned in 10‐μm thickness on a cryostat. Cryosections were stained with oil red O and hematoxylin and counterstained with fast green. Atherosclerotic lesion areas were measured with a Zeiss PrimoStar microscope connected with an Axiocam camera. The lesion areas of five sections with the largest readings were averaged for each mouse, and this average was used for statistical analysis.
Primo star microscope
Manufactured by Zeiss
Sourced in Germany, United States
The Primo Star microscope is a compact and robust educational microscope designed for basic microscopy applications. It features a reliable mechanical stage, coarse and fine focusing controls, and an Abbe condenser for uniform sample illumination. The Primo Star is suitable for a range of observation techniques, including bright-field and phase-contrast microscopy.
Automatically generated - may contain errors
Lab products found in correlation
Axiocam erc5, by Zeiss (7 mentions)
Pierce bca protein assay kit, by Thermo Fisher Scientific (4 mentions)
Axiocam 105, by Zeiss (2 mentions)
Axiocam erc5s digital camera, by Zeiss (2 mentions)
Axiocam erc5s camera, by Zeiss (2 mentions)
Zen 2, by Zeiss (2 mentions)
Infinite 200 pro series, by Tecan (2 mentions)
Axiocam camera, by Zeiss (1 mentions)
Jsm 6390lv, by JEOL (1 mentions)
Axiocam cc1, by Zeiss (1 mentions)
Eos 550d camera, by Canon (1 mentions)
Propidium iodide, by Vector Laboratories (1 mentions)
Fak antibody, by Cell Signaling Technology (1 mentions)
Vectashield mountant, by Vector Laboratories (1 mentions)
Prism 7, by GraphPad (1 mentions)
Sc 32757, by Santa Cruz Biotechnology (1 mentions)
Sc 7271, by Santa Cruz Biotechnology (1 mentions)
Mca341r, by Bio-Rad (1 mentions)
Entellan mounting medium, by Merck Group (1 mentions)
Vibratome, by Leica (1 mentions)
85 protocols using primo star microscope
1
Quantifying Aortic Atherosclerotic Lesions
2
Stomatal Aperture Analysis in Transgenic Arabidopsis
3
Cyphophthalmid Harvestmen Spermatopositor Morphology
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The specimens were collected during 15th–16th February 2014 through meticulous visual search throughout the floors of the forest and buildings. All specimens were captured with a fine brush and placed in vials containing 75% and 100% ethanol.
Nomenclature of body parts and measurements follows the model of Benavides and Giribet (2013) . Terminology of the structures of spermatopositor follows Juberthie (1970 ; 1979 ) and Karaman (2013) , with some modifications: (1) recognition of apical movable fingers (dma , digitus mobilis apicalis) which might not be homologous with dml (doigt mobile latéral) of Juberthie/Karaman, and (2) naming of three groups A, B, C of microtrichiae, hitherto unnamed, which are clearly recognizable also in other families of Cyphophthalmi 6 ).
The following abbreviations are used:MNRJ ; QCAZ ; MCZ .
Illustrations of the spermatopositor and ovipositor were made through a Carl Zeiss Primo Star microscope and AxioVision LE image capture system, with the stacking software Combine ZP Suite (by Alan Hadley). SEM images were made at JEOL JSM-6390 LV.
Nomenclature of body parts and measurements follows the model of Benavides and Giribet (2013) . Terminology of the structures of spermatopositor follows Juberthie (1970 ; 1979 ) and Karaman (2013) , with some modifications: (1) recognition of apical movable fingers (
The following abbreviations are used:
= Museu Nacional, Universidade Federal do Rio de Janeiro, Brazil
= Museo de Zoologia, Pontifícia Universidad Catolica del Ecuador – Quito, Ecuador
= Museum of Comparative Zoology, Harvard University, Cambridge, Massachusetts, USA
Illustrations of the spermatopositor and ovipositor were made through a Carl Zeiss Primo Star microscope and AxioVision LE image capture system, with the stacking software Combine ZP Suite (by Alan Hadley). SEM images were made at JEOL JSM-6390 LV.
4
Parasitological Analysis of Fecal Samples
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Faecal samples were analysed in the Laboratory of Parasitology of the National Institute of Tropical Medicine (INMeT) in Puerto Iguazú, Misiones, Argentina. We processed samples using a semi-quantitative flotation method with a saturated sugar solution55 (link). We weighted 3 g of faecal sample, then homogenised it, and after centrifugation we mounted it on a slide. We used a Carl Zeiss Primo Star Microscope to identify parasite structures and took pictures with Carl Zeiss AxioCam Cc1 using the 40x magnifier. Eggs and larvae were counted and identified on the basis of colour, shape, content and size. Although most samples reached 3 g after being processed, some samples did not reach this threshold. However, since some parasites were detectable even in < 3 g samples, instead of discarding them, we decided to consider faecal sample’s weight as a control factor in our analyses.
5
RORγ Overexpression Wound Healing
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The cells were plated in 6-well plates and cultured for 24 h and then subjected to the lentiviral transduction particles packing RORγ overexpression and control vector system for 12 h transfection. The scratches were produced across the cell monolayers with yellow tips. And the cells were washed with PBS solution for three times to remove the shedding cells. Then, the cells were cultured in fresh culture medium for another 48 h, and the confluence of cells was recorded with PrimoStar microscope (Zeiss, Jena, Germany). The migration index was calculated with the ratio of the scratch area between RORγ overexpression and vector cells by Image J software (US National Institutes of Health, Bethesda, MD, USA).
6
Microscopic Protein and Collagen Analysis
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The digital imaging system, comprising a Zeiss Primo Star microscope (Zeiss, Oberkochen, Germany) and a Canon EOS 550D camera (Canon, Tokyo, Japan), was used for image digitization. Ten HPFs were randomly captured per slide. I-solution program was used to quantify protein expression, BCL-2 and BAX immunolabeling protein expression was determined as the cancer area labeled intensity (μm2) in each slide, and an average was calculated. Moreover, Masson's Trichrome stained slide was also digitalized in 10 random HPFs. The blue staining areas were indicated as collagen formation area or fibrosis area and were measured by I-solution program (IMT, Canada). An average was calculated by Excel software (Microsoft, USA).
7
Immunofluorescence Microscopy of FAK
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For microscopy of FAK, cryosections were immunofluorescently labeled with primary antibodies used for IHC which were FAK Antibody (1:200; Cell Signaling) [27 (link)]. Antibody incubations were carried out at 4 °C in a humidified chamber, overnight. Bound antibody was visualized using anti-rabbit (1:50; Cell Signaling, USA) secondary antibody conjugated with fluorescein isothiocyanate. Fluorescently labeled sections were treated with 95% ethanol and then mounted under coverslips with Vectashield mountant containing propidium iodide (Vector Laboratories) before viewing under the microscope. Control sections were processed as above with omission of the primary antibodies. Microscopy was performed using a Zeiss Primo Star microscope.
8
Caudal Epididymal Sperm Counting
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The caudal epididymis was dissected from 8-week-old mice. Spermatozoa were squeezed out from the caudal epididymis and incubated for 30 min at 37 °C under 5% CO2. The incubated sperm medium was then diluted 1:10. A cover slip was placed on the hemocytometer before a drop with 10 μl of diluted caudal epididymal sperm solution was loaded under the cover slip. The hemocytometer was placed under the Primo Star microscope (Zeiss) and viewed under ×400 magnification. The microscope was not equipped with camera or software, and the light source of the microscope is LED: 3 W 3200k. Sperm count was done by counting 4 × 4 squares (horizontally or vertically) using the hemocytometer and calculated using the formula: Sperm count = total no. of sperm in 5 squares × 50,000 × dilution multiple (cells/ml). Counting was only done for sperm tails that was found within the square areas. The total number of sperm was counted and the mean was calculated from three counts. Six independent experiments were performed. The data were then analyzed with GraphPad Prism 7.
9
Parasitemia Quantification Protocol
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Slides for the experiments were counted on a light microscope using 100X objective with oil immersion with a 1:4 reticle on a Zeiss Primo Star microscope. The slides were counted and accessed for stages using the whole field method [41 (link)] across multiple fields (15–20 fields) until 500 red blood cells were counted in the small box of the 1:4 reticle. The slides for the different time points during the experiment were prepared using a cytospin (Shandon/Thermo Scientific) with 100 µL 10% (w/v) bovine serum albumin in PBS. Parasitaemia was calculated as: where the whole field factor for the 1:4 reticle was 10.9.
10
Counting Hemocytes in Invertebrate Immune Assay
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The number of circulating haemocytes was determined according to Urbański et al. [43 (link)]. Twenty-four hours after injection of EXT, the hemolymph sample (2 µL) was gently mixed with the 20 µL of the physiological saline solution and the anticoagulation buffer (4.5 mM citric acid and 9 mM sodium citrate; 5:1, v/v) and then placed on the Bürker chamber. The photos of 24 randomly selected squares were taken with a Zeiss Primostar microscope equipped with Axiocam 105 digital camera (Zeiss, Jena, Germany). To evaluate the number of haemocytes on the photos, cells were counted using ImageJ software (version 2., public domain, https://imagej.net/software/imagej2/ (accessed on 20 October 2022)). At least 13 individuals were used per treatment (Control—16, EXT 0.01%—14 and EXT 0.1%—13).
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