The release rates of LDH (C0016; Beyotime Biotechnology Co., Ltd.), Scr (C011-2; Nanjing Jiancheng Bioengineering Institute, Nanjing, Jiangsu, China) and BUN (C013-2; Nanjing Jiancheng Bioengineering Institute) were measured with corresponding test kits; IL-1β (SEA563Mu; Wuhan USCN Business Co., Ltd., Wuhan, China) and IL-18 (SEA064Mu; Wuhan USCN Business Co., Ltd.) were measured with enzyme-linked immunosorbent assay kits; and caspase-1 activity was measured with the Caspase 1 Activity Assay kit (C1101; Beyotime Biotechnology Co., Ltd.). All tests were performed according to the manufacturer’s instructions.
Il 1β
Manufactured by USCN
Sourced in China
IL-1β is a cytokine that plays a central role in the regulation of immune and inflammatory responses. It is produced by a variety of cell types, including macrophages, dendritic cells, and endothelial cells, in response to various stimuli such as infection, injury, or autoimmune processes.
Automatically generated - may contain errors
Lab products found in correlation
Il 18, by USCN (2 mentions)
C0016, by Beyotime (1 mentions)
Caspase 1 activity assay kit, by Beyotime (1 mentions)
Sea563mu, by USCN (1 mentions)
Oxiselect nitrotyrosine elisa kit, by Cell Biolabs (1 mentions)
Oxiselect tbars assay kit, by Cell Biolabs (1 mentions)
Polytron homogenizer pt 1200 e, by Kinematica (1 mentions)
Interleukin 6 (il 6), by USCN (1 mentions)
Il 10, by USCN (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Cytiva (1 mentions)
Penicillin, by Cytiva (1 mentions)
Streptomycin, by Cytiva (1 mentions)
C parp, by Cell Signaling Technology (1 mentions)
Brain derived neurotrophic factor (bdnf), by Promega (1 mentions)
Tnf α, by Thermo Fisher Scientific (1 mentions)
5 protocols using il 1β
1
Biochemical Markers of Cell Injury
2
Lung Cytokine and Oxidative Stress Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Concentrations of cytokines and oxidative modification products were determined in 10% (weight/volume) lung homogenate prepared using 0.1 M ice-cold phosphate buffer (PBS, pH 7.4) by Polytron homogenizer PT 1200 E (5-times for 25 s, 1200 rpm; Kinematica AG, Switzerland). Homogenates were then frozen 3 times and centrifuged (12,000 rpm, 15 min, 4 °C). Final supernatants were then stored at −70 °C until the analysis was performed.
Concentrations of IL-6, IL-1β, IL-10 (USCN Life Science Inc., Wuhan, China), and RAGE (MyBioSource, San Diego, CA, USA) were quantified using rabbit-specific ELISA kits according to the manufacturers’ instructions. Data were expressed in pg/mL.
Protein oxidative damage was determined using OxiSelectTM Nitrotyrosine ELISA kit (Cell Biolabs, Inc., San Diego, CA, USA). Data were expressed in 3-nitrotyrosine nanomole concentration (nM, 3NT). Lipid oxidative damage expressed by the concentration of thiobarbituric acid reacting substances (TBARS) was determined by OxiSelectTM TBARS Assay Kit (Cell Biolabs, Inc., San Diego, CA, USA). Data were expressed as malondialdehyde in micromole concentration (μM MDA).
Total antioxidant capacity (TAC) was determined using an ELISA kit according to the manufacturer’s instructions (Cell Biolabs, Inc., San Diego, CA, USA). Data were expressed as micromole concentration of copper reducing equivalents (μM CRE).
Concentrations of IL-6, IL-1β, IL-10 (USCN Life Science Inc., Wuhan, China), and RAGE (MyBioSource, San Diego, CA, USA) were quantified using rabbit-specific ELISA kits according to the manufacturers’ instructions. Data were expressed in pg/mL.
Protein oxidative damage was determined using OxiSelectTM Nitrotyrosine ELISA kit (Cell Biolabs, Inc., San Diego, CA, USA). Data were expressed in 3-nitrotyrosine nanomole concentration (nM, 3NT). Lipid oxidative damage expressed by the concentration of thiobarbituric acid reacting substances (TBARS) was determined by OxiSelectTM TBARS Assay Kit (Cell Biolabs, Inc., San Diego, CA, USA). Data were expressed as malondialdehyde in micromole concentration (μM MDA).
Total antioxidant capacity (TAC) was determined using an ELISA kit according to the manufacturer’s instructions (Cell Biolabs, Inc., San Diego, CA, USA). Data were expressed as micromole concentration of copper reducing equivalents (μM CRE).
3
Evaluating Inflammatory Cytokine Modulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dex, psoralen (National Institute for the Control of Pharmaceutical and Biological Products, Beijing, China); NP (Beijing Tong-ren-tang Technology Development Co., Ltd., Beijing, China); cigarettes (China Tobacco Guangdong Industrial Co., Ltd., Guangzhou, China); RPMI-1640 medium, penicillin, streptomycin (Hyclone Laboratories, Logan, UT, USA); and foetal bovine serum (FBS) (Gibco, USA) were used in this study. ELISA kits for tumour necrosis factor-α (TNF-α), interleukin-8 (IL-8), interleukin-6 (IL-6), and interleukin-1β (IL-1β) (USCN Life Science, Wuhan, China) were employed. In addition, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) (Sigma, USA) was also utilized.
4
Quantifying Hippocampal Markers in Alzheimer's
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Seven (synaptophysin), 8 (GFAP), or 9 (Aβ1–42, cPARP, BDNF, IL-1β, TNFα) days after Aβ25–35 or Sc.Aβ injection, hippocampi were dissected out and rinsed in ice-cold PBS to remove excess blood, and weighed before nitrogen freezing and −80°C storage. Tissues were cut into small pieces, homogenised, sonicated and centrifuged. The supernatants were assayed immediately by ELISA for GFAP, synaptophysin, IL-1β (USCN, China), TNFα (Thermo Scientific, France), BDNF (Promega, France), cPARP (Cell Signalling, France) and Aβ1–42 (Euromedex, France) following manufacturer's recommendations. All samples were assayed in duplicate and only samples for which the CV was <25% between replicates were included for analyses. Results were expressed in pg protein per mg of brain tissue, or as % of Sc. control. Protein concentration was determined in brain homogenates with the BCA protein assay kit (Pierce Perbio Science, France).
5
Measuring IL-1β and IL-18 Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell contents of IL-1β (USCN, Wuhan, China) and IL-18 (USCN, Wuhan, China) were determined by using ELISA kits following the manufacturer's instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!