Optima lc ms grade acetonitrile

Manufactured by Thermo Fisher Scientific

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Optima LC/MS grade acetonitrile is a high-purity solvent suitable for use in liquid chromatography-mass spectrometry (LC/MS) applications. It is designed to meet the stringent requirements of LC/MS analysis, providing a consistent and reliable performance.

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Acetonitrile (2707 citations) Optima grade (92 citations) LC-MS grade (75 citations) Optima LC/MS (67 citations) MS-grade acetonitrile (58 citations) Optima grade acetonitrile (23 citations) LC grade acetonitrile (7 citations)

49 protocols using optima lc ms grade acetonitrile

Glycoproteome Enrichment and Analysis

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Fetal bovine serum was obtained from Atlanta Biologicals (Lawrenceville, GA). Tris-HCl, PBS, aniline, iodoacetamide, dithiothreitol (DTT), urea, protease inhibitor cocktail solution, sodium(meta)periodate, Triton X-100, SDS, NH₄HCO₃, and glycerol were purchased from Sigma-Aldrch (St. Louis, MO). NaCl and trifluoroacetic acid was purchased from EMD chemicals (Gibbstown, NJ). Trypsin was obtained from Promega (Madison, WI). PNGaseF and G7 buffer were purchased from New England Biolabs (Ipswich, MA). High capacity streptavidin beads were obtained from Thermo Scientific (Rockford, IL). Amino-oxy-biotin was purchased from Biotium (Hayward, CA). Optima LC/MS grade acetonitrile and formic acid were purchased from Fisher Scientific. Pierce C18 spin columns were purchased from Glygen Corp (Columbia, MD). Fluorescently labeled monoclonal antibodies to murine Gr1 (clone RB6-8C5), CD11b (clone M170), CD47 (clone miap301), and isotype-matched controls were from BioLegend (San Diego, CA). Deionized water used for all experiments was obtained from a Milli-Q A10 system.

Chauhan S., Danielson S., Clements V., Edwards N., Ostrand-Rosenberg S, & Fenselau C. (2016). Surface Glycoproteins of Exosomes Shed by Myeloid-Derived Suppressor Cells Contribute to Function. Journal of proteome research, 16(1), 238-246.

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Proteomic Analysis of Modified Proteins

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All reagents and solvents were purchased at the highest purity available. Cadaverine dihydrochloride, 2HP dihydrochloride, horseradish peroxidase (HRP), ethanol, 4-vinylpyridine, ammonium citrate, and citric acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). Boric acid, sodium borate, and N-acetyl-3,7-dihydroxyphenoxazine (Amplex™ Red Reagent) were obtained from Thermo Fisher Scientific (Lenexa, KS, USA). Optima™ LC/MS-grade acetonitrile, water, and formic acid were purchased from Fisher Scientific (Thermo Fisher Scientific). Sequencing grade trypsin, chymotrypsin, and glycerol-free peptidyl-N-glycosidase F (PNGase F) were purchased from Promega (Madison, WI, USA) and New England BioLabs (Ipswich, MA, USA), respectively. Deionized water with a resistivity of 18 MΩ∙cm was purified using a Millipore Direct-Q3 Water Purification System (Billerica, MA, USA).

Meier A.A., Go E.P., Moon H.J., Desaire H, & Mure M. (2022). Mass Spectrometry-Based Disulfide Mapping of Lysyl Oxidase-like 2. International Journal of Molecular Sciences, 23(11), 5879.

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Profiling Cytochrome P450 Metabolism

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Optima LC-MS grade acetonitrile, water, formic acid, and ammonium
formate used in liquid chromatography were purchased from Fisher Scientific
(Santa Clara, CA). BACs of various alkyl chain lengths (C₁₀-,
C₁₂-, C₁₄-, and C₁₆-BAC), the DHCR7
inhibitor AY9944, and all synthetic reagents and starting materials were from
Sigma-Aldrich (St. Louis, MO). Deuterated NMR solvents were from Cambridge
Isotopes Laboratories, Inc. (Andover, MA). Human liver tissues were obtained
from the School of Pharmacy Human Liver Bank at the University of Washington
(Seattle, WA). Human recombinant CYP enzymes individually-expressed in
Escherichia coli (EasyCYP Bactosomes) co-expressing human
cytochrome P450 reductase and, where indicated as +b₅, supplemented
with purified human cytochrome b₅ (CYPs 1A2, 2A6+b₅, 2A13,
2B6+b₅, 2C8+b₅, 2C9+b₅, 2C19+b₅,
2D6, 2E1+b₅, 2J2, 3A4+b₅, 3A5+b₅,
4A11+b₅, 4F2+b₅, and 4F3B+b₅) and membrane
protein from E. coli with empty expression plasmid (EasyCYP
Control Bactosomes) were from XenoTech, LLC (Lenexa, KS). Human recombinant
CYP4F12 containing human cytochrome P450 reductase and cytochrome b₅in insect microsomes isolated from baculovirus-transfected insect cells (CYP4F12
Supersomes) and negative control insect cell microsomes (Control Supersomes)
were from Corning (Corning, NY). NADPH co-factor was from Oriental Yeast (Tokyo,
Japan).

Seguin R.P., Herron J.M., Lopez V., Dempsey J.L, & Xu L. (2019). Metabolism of Benzalkonium Chlorides by Human Hepatic Cytochromes P450. Chemical research in toxicology, 32(12), 2466-2478.

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Carnitine Quantification by LC-MS

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Glacial acetic acid and HPLC grade water were purchased from Fisher Scientific (Agawam, MA, USA). Optima LC-MS grade acetonitrile, methanol, ethyl acetate and formic acid were also purchased from Fisher Scientific. The following unlabeled standards were available and purchased from Sigma (St. Louis, MO, USA): acetylcarnitine (C2:0-CN), propionylcarnitine (C3:0-CN), butyrylcarnitine (C4:0-CN), valerylcarnitine (C5:0-CN), hexanoylcarnitine (C6:0-CN), octanylcarnitine (C8:0-CN) and decanoylcarnitine (C10:0-CN). As shown in Table S1 in the supplementary materials, the following stable isotope standards were available and purchased from Sigma (St. Louis, MO, USA), as well: acetyl-d₃-L-carnitine hydrochloride (d₃-C2:0-CN), butyryl-L-carnitine-N-methyl-d₃ (d₃-C4:0-CN), octanoyl-L-carnitine-N-methyl-d₃ (d₃-C8:0-CN), and decanoyl-L-carnitine-N-methyl-d₃ (d₃-C10:0-CN).

Vera N.B., Coy S.L., Laiakis E.C., Fornace AJ J.r., Clasquin M., Barker C.A., Pfefferkorn J.A, & Vouros P. (2020). Quantitation of Urinary Acylcarnitines by DMS-MS/MS Uncovers the Effects of Total Body Irradiation in Cancer Patients. Journal of the American Society for Mass Spectrometry, 31(3), 498-507.

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LC-MS Grade Reagents and Agar Plates

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The Optima LC-MS grade Water (H2O), Optima LC-MS grade acetonitrile (ACN), Optima LC-MS grade formic acid (FA) were purchased from Fisher Scientific (Fair Lawn, NJ). 70% Ethanol was purchased from in-house reagent laboratory and 7H11 agar plates from Hardy Diagnostics (Santa Maria, CA).

Bajaj A.O., Slechta E.S, & Barker A.P. (2022). Rapid and Accurate Differentiation of Mycobacteroides abscessus Complex Species by Liquid Chromatography-Ultra-High-Resolution Orbitrap™ Mass Spectrometry. Frontiers in Cellular and Infection Microbiology, 12, 809348.

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Quantification of Deuterium-Labeled Ibrutinib and Metabolites

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Ibrutinib (free base; I‐3311) was purchased from LC Laboratories. Deuterium‐labeled ibrutinib ([²H₅]ibrutinib, d₅‐ibrutinib, 22,561) and PCI 45227 (M37) were purchased from Cayman Chemical. Midazolam (M‐908), 1′‐hydroxymidazolam (H‐902), and d₄‐1′‐hydroxymidazolam (H‐921) were purchased from Cerilliant Corporation. The 4β‐HC (700036) and cholesterol (700000P) were purchased from Avanti Polar Lipids. Deuterium‐labeled 4β‐HC ([²H₇]4β‐HC, d₇‐4β‐HC, H917982) and deuterium‐labeled cholesterol ([²H₇]cholesterol, d₇‐cholesterol, C432503) were purchased from Toronto Research Chemicals. Dimethyl sulfoxide (DMSO), Optima liquid chromatography mass spectrometry LC–MS grade water, Optima LC–MS grade acetonitrile, Optima LC–MS grade methanol were obtained from Fisher Scientific. All other chemicals and reagents were of analytical grade or higher.
As described previously,²³ an NADPH‐regenerating system, consisting of solution A (26 mM NADP⁺, 66 mM glucose 6‐phosphate, 66 mM MgCl₂ in water) and solution B (40 U/ml glucose 6‐phosphate dehydrogenase in 5 mM sodium citrate), was purchased from Corning Life Sciences. InVitroGRO Krebs–Henseleit buffer (KHB) medium (product no. Z99074) and thawing (HT) medium (product no. 990006) were purchased from BioIVT.

Lee J., Fallon J.K., Smith P.C, & Jackson K.D. (2022). Formation of CYP3A‐specific metabolites of ibrutinib in vitro is correlated with hepatic CYP3A activity and 4β‐hydroxycholesterol/cholesterol ratio. Clinical and Translational Science, 16(2), 279-291.

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Analytical Protocols for Food Ingredients

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The following tests substances were purchased at local markets (Berkeley Bowl, Costco, Safeway, Whole Foods): potato, quinoa, and whole-wheat flours; Red Delicious and Fuji nonorganic and organic apples; Mandarin, Valencia, and navel oranges; Garnet and purple sweet potato yams; Galia organic and nonorganic melons; pepino melons; and red cherry tomatoes. Umatilla Russet frozen potato peels, a byproduct of the industrial preparation of French fries, were a gift from Dr. Jeff Bohlscheidt of the Simplot Company (Caldwell, ID, USA). Lion’s Mane, Reishi, and Turkey Tail mushroom powders of unknown origin were obtained from an online Healing Spirits Herb Farm and Education Center (Avoca, NY, USA). Acrylamide (≥99% purity) and Acrylamide-d3 solution (500 mg/L in acetonitrile) were purchased from Millipore Sigma (St. Louis, MO, USA). Optima LC/MS grade acetonitrile, methanol, and formic acid, and HPLC grade hexane were purchased from Fisher Scientific (Fair Lawn, NJ, USA).

Crawford L.M., Kahlon T.S., Wang S.C, & Friedman M. (2019). Acrylamide Content of Experimental Flatbreads Prepared from Potato, Quinoa, and Wheat Flours with Added Fruit and Vegetable Peels and Mushroom Powders. Foods, 8(7), 228.

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Quantification of Cyanotoxins in Aquatic Samples

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Lyophilized analytical standards of microcystin-LA, LF, LR, LW, LY, RR, YR, and nodularin, all with manufacturer reported purities of ≥95%, were purchased from Enzo Life Sciences, Inc. (Framingdale, New York, USA). Stock solutions were prepared at concentrations of 10 μg/mL in methanol. Certified reference materials (CRMs) of microcystin-LR, RR, and nodularin, in methanol, as well as anatoxin-a, cylindrospermopsin, and saxitoxin were purchased from the National Research Council Canada (Ottawa, Ontario, Canada). All MC standards were stored in the dark at −20 °C. All other standards were stored according to the manufacturer's recommendations. For LC-MS/MS and PPIA analyses, Optima LC/MS Grade acetonitrile, methanol, and water were purchased from Fisher Scientific (Pittsburgh, PA, USA) and used for sample preparation and LC eluents. Trifluoroacetic acid (Chromasolv^® for HPLC, >99.0%) and Tween 20 for PPIA extractions were purchased from Sigma Aldrich (St. Louis, MO, USA).

Marsan D.W., Conrad S.M., Stutts W.L., Parker C.H, & Deeds J.R. (2018). Evaluation of microcystin contamination in blue-green algal dietary supplements using a protein phosphatase inhibition-based test kit. Heliyon, 4(3), e00573.

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Metabolite Quantification by LC-MS

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Optima LC/MS grade acetonitrile, methanol and water, N,N-dimethylformamide (DMF), and 4-(4, 6-dimethoxy-1, 3, 5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) were purchased from Fisher Scientific (Fair Lawn, NJ). N-methylmorpholine (NMM) was purchased from TCI America (Tokyo, Japan). 12 Metabolite standards (histidine, valine, tyrosine, leucine, lysine, phenylalanine, tryptophan, alanine, serotonin, dopamine, γ-aminobutyric acid, and norepinephrine), 3-aminoquinoline, and triethylammonium bicarbonate (TEAB) was purchased from Sigma (St. Louis, MO). Formic acid (FA) (≥98%) was obtained from Fluka (Büchs, Switzerland). C18 and OMIX SCX Ziptips were purchased from Millipore and Agilent, respectively. Deionized water (18.2 MΩ·cm) was prepared with a Milli-Q Millipore system (Billerica, MA).

Hao L., Zhong X., Greer T., Ye H, & Li L. (2015). Relative quantification of amine-containing metabolites using isobaric N,N-dimethyl-leucine (DiLeu) reagents via LC-ESI-MS/MS and CE-ESI-MS/MS. The Analyst, 140(2), 467-475.

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Solvents for LC-MS Analysis

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Optima LC-MS grade Water (H₂O), Optima LC-MS grade acetonitrile (ACN), and Optima LC-MS grade formic acid (FA) were purchased from Fisher Scientific (Fair Lawn, NJ).

Bajaj A.O., Saraswat S., Knuuttila J.E., Freeke J., Stielow J.B, & Barker A.P. (2021). Accurate Identification of Closely Related Mycobacterium tuberculosis Complex Species by High Resolution Tandem Mass Spectrometry. Frontiers in Cellular and Infection Microbiology, 11, 656880.

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