Ab135940

Proteins extracts (40 μg) from striatum, motor cortex and cerebellum were separated on 4–12%-SDS polyacrylamide gels (Novex Pre-Cast gels, Invitrogene) and transferred to nitrocellulose membranes with a TransBlot cell apparatus (Bio-Rad). α-POL β (ab26343; 1:500, Abcam, Cambridge, UK), α-FEN1 (Abcam, Ab17993; 1:500), α-OGG1, (Abcam, Ab135940; 1:500), α-p53R2, (Abcam, Ab8105; 1:1000), APE1/Ref1 (sc5572; 1:100, Santa Cruz Biotechnology, USA), α-MUTYH (Abcam, Ab55551; 1:500) primary antibodies were used with α-β-Tubulin (Sigma, T5293; 1:2000) and α-LAMIN B1 (Abcam, ab16048; 1:10 000). Immunoreactions were detected with appropriate α-rabbit or α- mouse peroxidase-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, 1:10 000) and the ECL Western blotting detection Kit (Advansta). Signals were captured with ChemiDoc XRS system (Biorad) and quantified with Image Lab software.

The histone peptides were purchased from Chinese Peptide Company, all the primers and DNA substrates used in this paper was synthesized by GenScript, Inc. Four deoxynucleotide triphosphates (dNTPs) were purchased from New England Biolabs (N0446S). [γ-³²P]-ATP (BLU002A, 250 μCi) and [α-³²P]-dCTP (NEG513H, 250 μCi) were purchased from PerkinElmer. The M2 beads and M-280 beads were purchased from Sigma-Aldrich. The DNA Ligase I was purchased from Abnova. The antibodies used in this paper are as follow: anti-FEN1 (GTX70185, Genetex), anti-PRMT5 (sc-376937, Santa Cruz), anti-8-oxoG (sc-130914, Santa Cruz), anti-H4 (PTM-1003, PTM Bio), anti-H4R3me1 (ab17339, Abcam), anti-H4R3me2s (ab5823, Abcam), anti-OGG1 (ab135940, Abcam), anti-Flag (AP0007MH, Bioword), anti-GAPDH (AP0063, Bioworld), anti-Tubulin (AM031A, Abgent).