For TNF-α secretion analyses, macrophages (5 × 104 cells/96-well) were stimulated with LPS, or LPS and IL-10 for 6 h. Supernatants of stimulated macrophages were analyzed for TNF-α proteins with an enzyme-linked immunosorbent assay (ELISA) Kit (Invitrogen). The analysis was performed according to the manufacturer’s protocol with SpectraMax 340PC (Molecular Devices, San José, CA, USA).
Bio plex pro human cytokine 27 plex assay kit
The Bio-Plex Pro Human Cytokine 27-plex Assay kit is a multiplex immunoassay that allows for the simultaneous quantification of 27 different human cytokines and chemokines in a single well. The kit utilizes magnetic beads coated with specific antibodies to capture and detect the target analytes.
Lab products found in correlation
23 protocols using bio plex pro human cytokine 27 plex assay kit
Cytokine Secretion in Macrophages upon LPS and IL-10 Treatment
For TNF-α secretion analyses, macrophages (5 × 104 cells/96-well) were stimulated with LPS, or LPS and IL-10 for 6 h. Supernatants of stimulated macrophages were analyzed for TNF-α proteins with an enzyme-linked immunosorbent assay (ELISA) Kit (Invitrogen). The analysis was performed according to the manufacturer’s protocol with SpectraMax 340PC (Molecular Devices, San José, CA, USA).
Secretome Analysis of Cytokines
Multiplex Serum Cytokine Analysis
Cytokine and MMP Response to Cigarette Smoke
Multiplex Cytokine Quantification in Plasma and PBMC
Biomarker Panel for Neurodegenerative Conditions
Traditional and automated enzyme-linked immunosorbent assays will be run to quantify serum levels of amyloid beta (aa1-42) (#DAB142, R&D Systems, Minneapolis, MN, USA), neurofilament light polypeptide (#SPCKB-PS-002448-000190, R&D Systems), Tau (#NBP2-62749, Novus Biologicals, Littleton, CO, USA), and Tau [p Ser739] (#NBP2-66711, Novus Biologicals) proteins according to the manufacturer’s instructions.
Quantifying Cytokine Levels in SFTS Serum
Quantifying Cytokine and MMP Secretion
Multiplex Cytokine Profiling in Fecal Samples
Modulation of Cytokine/Chemokine Response in Respiratory Cell Lines
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