MDA-MB-231, Hs578T, BT20, MCF7, and H1299 cells were purchased from Korea Cell Line Bank (Seoul, Korea). MCF10A cells were obtained from the American Type Culture Collection (Manassas, VA, USA). MDA-MB-231, BT20 and MCF7 cells were maintained in RPMI 1640 medium (Invitrogen, Carlsbad, CA, USA), supplemented with 10% (v/v) fetal bovine serum (FBS; Biowest, Nuaillè, France), 100 units/mL penicillin, and 100 μg/mL streptomycin (Welgene, Korea). Hs578T cells were maintained in Dulbecco’s Modified Eagle’s Medium (Invitrogen, Carlsbad, CA, USA), supplemented with 10% (v/v) FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin. MCF10A cells were cultured in DMEM/F12 medium (Invitrogen, Carlsbad, CA, USA) containing 10 μg/mL bovine insulin, 20 ng/mL epidermal growth factor, 100 ng/mL cholera enterotoxin, 0.5 μg/mL hydrocortisone (Sigma, St. Louis, MO, USA), and 5% horse serum (Welgene, Korea).
Hs578t
Manufactured by Korean Cell Line Bank
Sourced in United States
Hs578T is a human breast cancer cell line derived from a primary carcinoma of the breast. It is a well-characterized and widely-used cell line for cancer research.
Automatically generated - may contain errors
Lab products found in correlation
Mda mb 231, by Korean Cell Line Bank (6 mentions)
Mda mb 231, by American Type Culture Collection (5 mentions)
Mcf 7, by Korean Cell Line Bank (4 mentions)
Sk br 3, by Korean Cell Line Bank (4 mentions)
Mcf 10a, by American Type Culture Collection (3 mentions)
Streptomycin, by Thermo Fisher Scientific (3 mentions)
Mda mb 453, by Korean Cell Line Bank (3 mentions)
Hcc 1395, by Korean Cell Line Bank (3 mentions)
Mcf 7, by American Type Culture Collection (3 mentions)
Zr 75 1, by Korean Cell Line Bank (3 mentions)
H1299, by Korean Cell Line Bank (2 mentions)
Mda mb 231, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Bt549, by American Type Culture Collection (2 mentions)
Bt 20, by American Type Culture Collection (2 mentions)
Bt 474, by Korean Cell Line Bank (2 mentions)
Mcf 7 cell, by American Type Culture Collection (2 mentions)
Dulbecco modified eagle medium (dmem), by Welgene (2 mentions)
Hcc1419, by Korean Cell Line Bank (2 mentions)
Hcc 70, by Korean Cell Line Bank (2 mentions)
17 protocols using hs578t
1
Culturing Diverse Breast Cancer Cell Lines
2
Comprehensive Breast Cancer Cell Line Cultivation
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Ten human BC cell lines (T47D, MCF-7, BT-474, SK-BR-3, MDA-MB-453, Hs578T, MDA-MB-231, HCC-1395, BT-20, and BT-549) and MCF-10A, a normal epithelial breast cell line, were used for this study. MCF-7, MDA-MB-231, BT-549, BT-20, and MCF-10A were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). T47D, BT-474, SK-BR-3, MDA-MB-453, Hs578T, and HCC-1395 were purchased from the Korean Cell Line Bank (Seoul National University, Seoul, Korea). T47D, BT-474, SK-BR-3, HCC-1395, and BT-549 were cultured in Roswell Park Memorial Institute (RPMI) medium supplemented with 10% fetal bovine serum (FBS) and 1× Antibiotic-Antimycotic (AA, Gibco, Carlsbad, CA, USA). MDA-MB-453, MDA-MB-231, and BT-20 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS and 1 × AA. Hs578T cells were cultured in DMEM supplemented with 10% FBS, 1 × AA, and 25 mM HEPES. MCF-7 was cultured in minimum essential medium (MEM) supplemented with 10% FBS and 1 × AA. MCF-10A cells were cultured in DMEM/F-12 medium supplemented with 5% horse serum, 1 × AA, 20 ng/mL hEGF, 0.5 mg/mL hydrocortisone, 100 ng/mL cholera toxin, and 10 µg/mL insulin. Cultures were maintained at 37 °C in a humidified atmosphere with 5% CO2.
3
CRISPR-Cas9 Mediated αTAT1 Knockout in MDA-MB-231 Cells
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MDA-MB-231 was kindly provided by Dr. JS Nam (Gwangju Institute of Science and Technology [GIST], Korea). αTAT1 KO MDA-MB-231 cells were generated using the CRISPR-Cas9 system as described in a recent paper (Ko et al., 2021 (link)). Briefly, Lentiviral particles expressing a guide sequence (5’-CATGAGTCTGTGCAACGCCA-3’) targeting Atat1 were produced by transfection of HEK293T cells with lentiCRISPR v2 plasmid, psPAX2, and pMD2.G using polyethylenimine for 48 h (Oh et al., 2017 (link)) MDA-MB-231 cells transduced with the lentivirus were selected with puromycin (1 μg/ml) for 2 weeks. Hs578t was purchased from the Korean Cell Line Bank. All cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, USA) with high glucose, 10% fetal bovine serum, 100 unit/ml penicillin, 100 μg/ml streptomycin, and 250 ng/ml amphotericin B (Gibco) in a humidified incubator at 37°C and 5% CO2. All cell lines were confirmed not to be infected with mycoplasma using e-Myco VALiD Mycoplasma PCR Detection kit (iNtRON, Korea).
4
Cell Line Cultivation and Authentication
5
Breast Cancer Cell Culture Protocol
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MCF10A, ZR75-30, MDA-MB-453, BT20, JIMT-1, MDA-MB-231, and HEK293T cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). MCF7, T47D, ZR75-1, SKBR3, BT474, HCC-1954, HCC1419, and HS578T cells were purchased from the Korean Cell Line Bank. MCF7, T47D, MDA-MB-231, and HEK293T cells were cultured in DMEM (Welgene, Daegu, Republic of Korea) supplemented with 10% FBS, and SKBR3, BT474, HCC-1954, HCC-1419, JIMT-1, and HS5788T cells were cultured in RPMI (Welgene) supplemented with 10% FBS at 37 °C in a 5% CO2 atmosphere. Trastuzumab was obtained from Roche (Basel, Switzerland). The β-Catenin/TCF inhibitor FH-535 was purchased from Sigma-Aldrich (St. Louis, MO, USA).
6
Culturing Breast and Ovarian Cancer Cell Lines
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The human breast cancer cell lines, Hs578T, T47D, MCF-7, and ovarian cancer cell lines, OVCAR-3 and Caov-3, were purchased from the Korean Cell Line Bank (KCLB; Seoul, Republic of Korea). The human ovarian cancer cell line TOV-112D was from American Type Culture Collection (ATCC; Manassas, VA, USA). TOV-112D cells were transfected for stable expression of CLDN3 as described in previous study43 . TOV-112D cells was cultured in 1:1 mixture of Media199/MCDB medium (HyClone, Logan, UT, USA) containing 15% FBS, 100 unit/mL penicillin, and 100 μg/mL streptomycin. T47D, MCF-7, OVCAR-3 cells were cultured in RPMI-1640 medium (HyClone) supplemented with 10% fetal bovine serum (FBS; HyClone), 100 U/mL penicillin, and 100 μg/mL streptomycin. Caov-3 and Hs578T cells were cultured in Dulbecco's modified Eagle's medium (DMEM, HyClone) supplemented with 10% FBS (HyClone), 100 U/mL penicillin, and 100 μg/mL streptomycin. The cells were incubated at 37 °C in a humidified 5% CO2 atmosphere.
7
Culturing TNBC Cell Lines
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Three human TNBC cell lines, MDA-MB-231, Hs578T, and MDA-MB-453, were used in this study. MDA-MB-231 cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA), and Hs578T cells and MDA-MB-453 cells were purchased from the Korean Cell Line Bank (Seoul National University, Seoul, Korea). MDA-MB-231 and MDA-MB-453 cells were cultured in Dulbecco′s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), and Hs578T cells were cultured in DMEM supplemented with 10% FBS and 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES). Cultures were maintained in a humidified atmosphere comprising 95% air and 5% CO2 at 37 °C.
8
Breast Cancer Cell Line Culture
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The breast cancer cell lines used were MDA-MB-231, MDA-MB-453, HCC70, BT-20, and Hs578T cells, which were purchased from the Korean Cell Line Bank (Seoul, Republic of Korea), and BT-549 cells, which were purchased from ATCC (Manassas, VA, USA). The cells were grown in a humidified atmosphere with 5% CO2 at 37 °C in either Dulbecco’s Modified Eagle Medium (DMEM) or RPMI Medium 1640 (RPMI), supplemented with 10% FBS and penicillin.
9
Culturing Human Breast Cancer Cells
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MDA-MB-231 and MCF-7 human breast cancer cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). HCC-1395, Hs578T, T47D, and MCF-10A cells were purchased from Korean Cell Line Bank (Seoul, Korea). MDA-MB-231, MCF-7, and Hs578T cells were cultured in DMEM high glucose supplemented with 10% FBS, 100 IU/ml penicillin, and 100 μg/ml streptomycin. HCC-1395, T47D, and MCF-10A cells were grown in RPMI1640 medium with 10% FBS, 100 IU/ml penicillin, and 100 μg/ml streptomycin. The cells were continuously maintained at 37 °C in a 5% CO2-humidified incubator.
10
Breast Cancer Cell Line Characterization
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The MCF7, MDA-MB-231, MDA-MB-436, and MDA-MB-157 cell lines were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA). The SK-BR-3, ZR75-1, Hs578T, BT20, HCC70, HCC1937, DU4475, HCC38, and T47D cell lines were obtained from the Korean Cell Line Bank (KCLB, Seoul, Korea). BT474 cells were kindly provided by Dr. Incheol Shin (Hanyang University, Seoul, Korea). These cells were cultured as described in the ATCC website (www.atcc.org ). The MDA-MB-231, MDA-MB-436, MDA-MB-453, MDA-MB-468, and Hs578T cell lines were cultured in DMEM (Gibco, CA, USA) containing 10% fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA) and 1% penicillin/streptomycin (Gibco). SBCC-1 and SBCC-2 cells (from invasive breast cancer) and the NDY-1 cell line (breast sarcoma) were established in our laboratory, as previously described [48 (link)]. All of the other cell lines were grown in RPMI 1640 supplemented with 10% FBS and 1% penicillin/streptomycin. Cells were maintained at 37°C in a humidified atmosphere of 95% air and 5% CO2, and periodically screened for mycoplasmic contamination.
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