Cl 1000

Manufactured by Analytik Jena

Sourced in United States, Germany, United Kingdom

The CL-1000 is a high-performance luminescence detection system designed for sensitive and accurate measurements. It features a highly sensitive photomultiplier tube detector and a variety of optical filters to support various luminescence applications.

Automatically generated - may contain errors

Lab products found in correlation

γ 32p atp, by PerkinElmer (3 mentions) Dma q800, by TA Instruments (3 mentions) α 32p deoxycytidine 5 triphosphate, by PerkinElmer (2 mentions) Amersham hybond n membrane, by GE Healthcare (2 mentions) Typhoon fla 7000, by GE Healthcare (2 mentions) Typhoon trio, by Cytiva (2 mentions) Trans blot turbo apparatus, by Bio-Rad (2 mentions) Perfecthyb, by Toyobo (2 mentions) Amersham hybond n, by GE Healthcare (2 mentions) Bas ms2040, by Fujifilm (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Pen strep, by Thermo Fisher Scientific (2 mentions) Northernmax kit, by Thermo Fisher Scientific (1 mentions) Pluronic f108, by Merck Group (1 mentions) Imagequant software, by GE Healthcare (1 mentions) Random primer dna labeling kit ver 2, by Takara Bio (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Dmem f12 glutamax 1, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CL-1000 Ultraviolet Crosslinker (40 citations) CL-1000 UV Crosslinker (25 citations) CL-1000 crosslinker (24 citations) CL-1000S UV crosslinker (4 citations) UVP Crosslinker CL-1000 (4 citations) UV Cross-linker CL-1000 (2 citations) Trans Linker CL-1000 (2 citations)

53 protocols using cl 1000

Acellular Dermal Matrix UV Crosslinking Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 2

A 2 cm×3 cm sample of sheet acellular dermal matrix (ADM) derived from porcine dermis and having a wet thickness of approximately 1.3 mm was vacuum dried at 35° C. at less than 100 millitorr absolute pressure for 12-24 hours, yielding a dried, semi-transparent ADM having a thickness of approximately 0.7 mm. The dried, semi-transparent ADM was then transferred to a UVP model CL-1000 ultraviolet crosslinker using UVA lamps emitting a UV wavelength in the range of from 350-375 nm (target 365 nm) and irradiated with 370 nm wavelength UV-A light at an intensity of 5.5 mW/cm²for 4 hours. The ADM was then rehydrated overnight in Dulbecco's phosphate-buffered saline (PBS buffer) with no Ca or Mg salts (standard nominal concentration of 2.67 mM KCl, 1.47 mM KH₂PO₄, 138 mM NaCl, and 8.06 mM Na₂HPO₄.7H₂O).

A 2 cm×3 cm sample of “wet” (i.e., not vacuum dried overnight) sheet acellular dermal matrix (ADM) derived from porcine dermis and having a wet thickness of approximately 1.3 mm was placed in a UVP model CL-1000 ultraviolet crosslinker using UVA lamps emitting a UV wavelength in the range of from 350-375 nm (target 365 nm) and irradiated with 370 nm wavelength UV-A light at an intensity of 5.5 mW/cm²for 4 hours.

US11179505B2. Methods for stabilizing collagen-containing tissue products against enzymatic degradation (2021-11-23). LifeCell Corporation [US]. Inventors: Israel Jessop [US], Ming F. Pomerleau [US], Nathaniel Bachrach [US].

+ Open protocol

+ Expand

Acellular Dermal Matrix UV Crosslinking

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 1

A 2 cm×3 cm sample of sheet acellular dermal matrix (ADM) derived from porcine dermis and having a wet thickness of approximately 1.3 mm was vacuum dried at 35° C. at less than 100 millitorr absolute pressure for 12-24 hours, yielding a dried, semi-transparent ADM having a thickness of approximately 0.7 mm. The dried, semi-transparent ADM was then transferred to a UVP model CL-1000 ultraviolet crosslinker using UVA lamps emitting a UV wavelength in the range of from 350-375 nm (target 365 nm) and irradiated with 370 nm wavelength UV-A light at an intensity of 5.5 mW/cm²for 2 hours. The ADM was then rehydrated overnight in Dulbecco's phosphate-buffered saline (PBS buffer) with no Ca or Mg salts (standard nominal concentration of 2.67 mM KCl, 1.47 mM KH₂PO₄, 138 mM NaCl, and 8.06 mM Na₂HPO₄.7H₂O).

+ Open protocol

+ Expand

HaCaT UVB Exposure and Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols

Before the experiment, 1 × 10⁴ HaCaT cells were plated on each well of a 96-well plate for 24 h. Then, cells were washed with PBS before adding a thin layer of PBS. Cells were then exposed to UVB light (500 mJ/cm²) using CL-1000 (UVP, Analytik Jena, Jena, Germany). Then, cells were maintained in serum-free DMEM with DMSO, EGF, or IPC for 24 h. Control cells were maintained without UVB irradiation.

Kwon P.K., Kim S.W., De R., Jeong S.W, & Kim K.T. (2021). Isoprocurcumenol Supports Keratinocyte Growth and Survival through Epidermal Growth Factor Receptor Activation. International Journal of Molecular Sciences, 22(22), 12579.

+ Open protocol

+ Expand

Northern Blot Analysis of RNA Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Northern blot was performed using NorthernMax kit (ThermoFisher Scientific) and the probes were labeled by α-³²P-deoxycytidine 5’-triphosphate (PerkinElmer) using Random Primer DNA Labeling Kit Ver. 2 (Takara, #6045) according to manufacturer’s protocols. Briefly, RNA samples (10 or 20 µg) were resolved in 1 % agarose gel by electrophoresis at 5 V/cm in 1× MOPS buffer for ~ 2 h. Then RNA was transferred onto an Amersham Hybond-N + membrane (GE Healthcare) by capillary blot for 2.5 h using the transfer buffer supplied in NorthernMax kit. Transferred RNA was crosslinked by 254 nm UV at 1200 × 100 µJ/cm² (Analytik Jena CL-1000). Prehybridization was performed in ULTRAHybe buffer at 50^oC for one hour, followed by hybridization with ³²P labeled probes overnight at 50^oC. The membrane was washed 2 × 5 min at room temperature using Low Stringency Washing Solution and 2 × 15 min at 50^oC using High Stringency Washing Solution. The membrane was sealed in kitchen wrap and exposed to a phosphorscreen for several hours to overnight, and the signals were detected by Typhoon FLA7000 (GE Healthcare). Primers used for probe amplification are listed in Table S2.

, & Cao D. (2021). Reverse complementary matches simultaneously promote both back-splicing and exon-skipping. BMC Genomics, 22, 586.

+ Open protocol

+ Expand

Monocyte Activation by H7N9-Infected Supernatant

Check if the same lab product or an alternative is used in the 5 most similar protocols

The culture supernatants of H7N9-infected monocytes with or without treatment were collected at 24 hpi and UV inactivated for 10 min with UVP ultraviolet crosslinker CL-1000 (Analytik Jena, CA, USA). The UV-inactivated supernatants were diluted 1:1 with fresh culture medium. Freshly isolated CD14⁺ monocytes were incubated with diluted supernatant for 72 h. Cells were then harvested for flow cytometry determination of cell surface expression of CD80, CD83 and CD86.

Lee A.C., Zhang A.J., Chu H., Li C., Zhu H., Mak W.W., Chen Y., Kok K.H., To K.K, & Yuen K.Y. (2019). H7N9 influenza A virus activation of necroptosis in human monocytes links innate and adaptive immune responses. Cell Death & Disease, 10(6), 442.

+ Open protocol

+ Expand

Organoid Cultivation in Hanging-Drop Plates

Check if the same lab product or an alternative is used in the 5 most similar protocols

For at least one day prior to organoid seeding, a 384-well hanging-drop (HD) plate custom designed with injection molding (Xcentric) was soaked in 0.1% solution of Pluronic F108 (Sigma-Aldrich, 542342) in distilled water (diH₂O) (Gibco, 15230). On the day of organoid seeding, the HD plate was rinsed off with diH₂O and then air dried. The plate was then UV-sterilized (Analytik Jena, CL-1000) for 20 minutes on each side. While waiting, the wells of a sterile non-tissue-treated round-bottom 96-well plate (Corning, 351177) were each filled with 150 μl of diH₂O supplemented with 1% pen-strep. When the HD plate was fully sterilized, it was placed (sandwiched) between the bottom and the lid of the 96-well plate. The two troughs of the short-edged sides were loaded with diH2O, which were then stuffed with sterile gauze pads. Lastly, the long edges were each added with ~ 1 ml of diH₂O.

Lee J.H., LeCher J.C., Parigoris E., Shinagawa N., Sentosa J., Manfredi C., Goh S.L., De R., Tao S., Zandi K., Amblard F., Sorscher E.J., Spence J.R., Tirouvanziam R., Schinazi R.F, & Takayama S. (2024). Stably-Inverted Apical-Out Human Upper Airway Organoids for SARS-CoV-2 Infection and Therapeutic Testing. bioRxiv.

+ Open protocol

+ Expand

SARS-CoV-2 Inactivation by UV-C Irradiation

Check if the same lab product or an alternative is used in the 5 most similar protocols

For inactivation, 50 µL of purified SARS-CoV-2 was exposed to UV-C irradiation at energies ranging from 6.25 to 100 mJ cm⁻² using a UVP cross-linker CL-1000 (Analytik Jena, Jena, Germany) at A₂₅₄ nm. SARS-CoV-2 samples used for cryoEM were inactivated with 100 mJ cm⁻². Infectivity reduction was assessed using plaque assays.

Plavec Z., Domanska A., Liu X., Laine P., Paulin L., Varjosalo M., Auvinen P., Wolf S.G., Anastasina M, & Butcher S.J. (2022). SARS-CoV-2 Production, Purification Methods and UV Inactivation for Proteomics and Structural Studies. Viruses, 14(9), 1989.

+ Open protocol

+ Expand

Functional Recovery of XPC-Deficient Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

To assess the functional recovery of GM14867 cells, which carry a homozygous mutation in the XPC gene, after treatment with xABE, ABEmax, ataluren, or gentamicin, these cells, together with BJ-5ta WT cells, were exposed to ultraviolet irradiation at 254 nm at 1 J/m²/s (cat. no. CL-1000, Analytik Jena) and left to grow for 72 h. Cells treated with ataluren or gentamicin underwent treatment for 48 h before ultraviolet exposure. Cell survival was evaluated with a water-soluble tetrazolium salt assay using an EZ-Cytox kit (cat. no. EZ-1000, DoGEN).

Lee C., Hyun Jo D., Hwang G.H., Yu J., Kim J.H., Park S.E., Kim J.S., Kim J.H, & Bae S. (2019). CRISPR-Pass: Gene Rescue of Nonsense Mutations Using Adenine Base Editors. Molecular Therapy, 27(8), 1364-1371.

+ Open protocol

+ Expand

Northern Blot Analysis of Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Northern blot was performed using NorthernMax kit (ThermoFisher Scienti c) and the probes were labeled by α-32 P-deoxycytidine 5'-triphosphate (PerkinElmer) using Random Primer DNA Labeling Kit Ver.
2 (Takara, #6045) according to manufacturer's protocols. Brie y, RNA samples (10 µg or 20 µg) were resolved in 1% agarose gel by electrophoresis at 5 V/cm in 1× MOPS buffer for ~ 2 hours. Then RNA was transferred onto an Amersham Hybond-N + membrane (GE Healthcare) by capillary blot for 2.5 hours using the transfer buffer supplied in NorthernMax kit. Transferred RNA was crosslinked by 254 nm UV at 1200 × 100 µJ/cm 2 (Analytik Jena CL-1000). Prehybridization was performed in ULTRAHybe buffer at 50 o C for one hour, followed by hybridization with 32 P labeled probes overnight at 50 o C. The membrane was washed 2 × 5 min at room temperature using Low Stringency Washing Solution and 2 × 15 min at 50 o C using High Stringency Washing Solution. The membrane was sealed in kitchen wrap and exposed to a phosphorscreen for several hours to overnight, and the signals were detected by Typhoon FLA7000 (GE Healthcare). Quanti cation of bands was performed using ImageQuant software (GE Healthcare). Primers used for probe ampli cation are listed in Table S2.

Cao D. (2021). Reverse complementary matches simultaneously promote both back-splicing and exon-skipping.

+ Open protocol

+ Expand

UVB-Induced Apoptosis Screening

Check if the same lab product or an alternative is used in the 5 most similar protocols

1 × 10⁴ HaCaT cells were plated with DMEM supplemented with 10% FBS and 1% penicillin-streptomycin on 96-well plate and incubated for 24 hr. Cells were washed with PBS three times and thin layer of PBS was added. Cells were then exposed to UVB light (35 and 50 mJ/cm2) using 312 nm light source of CL-1000 (UVP). After UVB irradiation, cells were cultured in serum free DMEM with or without compounds for 24 hr. Control cells were identically processed but without UVB irradiation.

Lee D., Lim J., Woo K.C, & Kim K.T. (2018). Piperonylic acid stimulates keratinocyte growth and survival by activating epidermal growth factor receptor (EGFR). Scientific Reports, 8, 162.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!