Azoxymethane (Sigma, United States), dextran sulfate sodium (36–50 kDa, MP Biomedicals, United States), phosphate-buffered saline (Hyclone, United States), PageRuler Plus Prestained Protein Ladder (ThermoFisher, United States), TMT® Mass Tagging Kits and Reagents (ThermoFisher, United States), dithiothreitol (Sigma, United States), iodoacetamide (Sigma, United States), sodium dodecyl sulfate (Sigma, United States), urea (Sigma, United States), pancreatin (Promega, China), ammonium bicarbonate (Sigma, United States), UltraPure water (ThermoFisher, United States), triethylammonium bicarbonate buffer (Sigma, United States), acetonitrile (ThermoFisher, United States), formic acid (ThermoFisher, United States), acetone (ThermoFisher, United States), ProteoMiner Low-Abundance Protein Enrichment Kit (Bio-Rad, United States), and trifluoroacetate (Sigma, United States) were used in this study.
Pancreatin
Manufactured by Promega
Sourced in United States
Pancreatin is a mixture of digestive enzymes produced by the pancreas. It contains amylase, lipase, and protease enzymes that aid in the breakdown of carbohydrates, fats, and proteins, respectively. Pancreatin is commonly used in research and laboratory settings to facilitate the digestion and processing of various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Formic acid, by Thermo Fisher Scientific (3 mentions)
Acetonitrile, by Thermo Fisher Scientific (3 mentions)
Iodoacetamide, by Merck Group (2 mentions)
Urea, by Merck Group (2 mentions)
Dithiothreitol (dtt), by Merck Group (2 mentions)
Ultrapure water, by Thermo Fisher Scientific (1 mentions)
Azoxymethane, by Merck Group (1 mentions)
Triethylammonium bicarbonate buffer, by Merck Group (1 mentions)
Trifluoroacetate, by Merck Group (1 mentions)
Tmt mass tagging kits and reagents, by Thermo Fisher Scientific (1 mentions)
Proteominer low abundance protein enrichment kit, by Bio-Rad (1 mentions)
Acetone, by Thermo Fisher Scientific (1 mentions)
Pageruler plus prestained protein ladder, by Thermo Fisher Scientific (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Ammonium bicarbonate, by Merck Group (1 mentions)
Dextran sulfate sodium (dss), by MP Biomedicals (1 mentions)
Multiskan spectrum, by Thermo Fisher Scientific (1 mentions)
Dionex ultimate 3000 rslcnano, by Thermo Fisher Scientific (1 mentions)
Q exactive, by Thermo Fisher Scientific (1 mentions)
Itraq 8 plex kit, by AB Sciex (1 mentions)
3 protocols using pancreatin
1
Proteomic Analysis of Colorectal Cancer
2
Multimodal Proteomic Profiling of Human Immune Cells
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The ADVIA® 120 Hematology System and Full Blood Count Test Kit were from Siemens, Germany. BD FACSCalibur, and FITC mouse anti-human CD3, APC mouse anti-human CD4+, and PE mouse anti-human CD8+, APC mouse IgG1, ƙ isotype control, PE mouse IgG1, ƙ isotype control, FITC mouse IgG1 ƙ isotype control, and CalibriteE 3-color kit were from BD Biosciences, USA. 8-plex iTRAQ Kit was from AB Sciex, USA. Pancreatin was from Promega, USA. First-dimension high pH-RP liquid chromatography was measured via Shimadzu HPLC, USA. Second-dimension liquid chromatography was measured via Thermo Dionex Ultimate 3000 RSLCnano, USA. Mass Spectrometer was performed on a Thermo Scientific Q Exactive, USA. The ELISA Test Kits of SEMG-1, PIP, PDGFRL, and NDPK were from Shanghai Enzyme-linked Biotechnology Co., Ltd., China. The Multiskan Spectrum was from Thermo Scientific, USA.
3
Traditional Chinese Herbal Medicine Evaluation
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Medicinal herbs: The Chinese herbal medicine used in this study included Acanthopanax senticosus (Rupr. Maxim.) harms (purchased from Wuchang County, Heilongjiang Province, with a growth period of six years), Cynanchum otophylum (purchased from Haozhou City, Anhui Province), and Uncaria rhynchophylla (Miq.) Miq. ex Havil (purchased from Jianhe County, Guizhou Province).The TUNEL kit (Jiancheng), BCA protein concentration assay kit (Solarbio), RIPA lysis buffer (Solarbio), 5 × protein loading buffer (Biosntech), SDS‒PAGE gel preparation kit (Biosharp), Tris Base (Sigma), sodium dodecyl sulfate (Nachuan), special nonfat milk powder for sealing (Pulilai Gene), Tween 20 (BIOFROXX), western secondary antibody removal solution (Beyotime), hypersensitive ECL luminescent liquid (Meilunbio), LC3B (Sanying), P53 (Abcam), AMPK (Abcam), mTOR (Abcam), BAX (Boaosen), Caspase-3 (Boaosen), GAPDH (Boaosen), protease inhibitor (Calbiochem), pancreatin (Promega), acetonitrile (Fisher Chemical), trifluoroacetic acid (Sigma Aldrich), formic acid (FLUKA), iodoacetamide (Sigma), dithiothreitol (Sigma), urea (Sigma), triethylammonium bicarbonate (Sigma), purified water (Fisher Chemical), BCA Kit (Biyuntian), TMT labeling kit (Thermo), and phosphorylase inhibitor (Millipore) were the chemicals used in our study.
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