Qm100a 1

Manufactured by System Biosciences

Sourced in United States

The QM100A-1 is a laboratory instrument designed for quantitative measurement of fluorescent and luminescent signals. It offers accurate and reproducible detection of a wide range of fluorescent and luminescent molecules. The core function of the QM100A-1 is to provide reliable quantitative data analysis for research applications.

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Lab products found in correlation

Fetal bovine serum (fbs), by Takara Bio (1 mentions) Cumate, by System Biosciences (1 mentions) Screenfect a, by Fujifilm (1 mentions) Dulbecco modified eagle medium (dmem), by Fujifilm (1 mentions) Cmir0001 mr04, by GeneCopoeia (1 mentions) Miexpress egfp mmu mir 155 plasmid, by GeneCopoeia (1 mentions) Mmir3427 mr04, by GeneCopoeia (1 mentions) Chir99021, by STEMCELL (1 mentions)

4 protocols using qm100a 1

Inducible Gene Expression in U2OS Cells

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U2OS cells stably expressing (ArrayG/N)_16X, mwGFP, and sgRNA were cultured at 37°C and 5% CO₂ in high-glucose DMEM (HyClone) supplemented with 10% (vol/vol) fetal bovine serum (FBS) (ClonTech), 250 µg/ml hygromycin, 1 µg/ml puromycin, and 10 µg/ml blasticidin. Expression of (ArrayG/N)_16X was induced by 1× doxycycline (1 µg/ml), and mwGFP was induced by 1× cumate (QM100A-1; System Biosciences) 24–48 h before imaging. sgRNA was constitutively expressed.

Gustavsson A.K., Ghosh R.P., Petrov P.N., Liphardt J.T, & Moerner W.E. (2022). Fast and parallel nanoscale three-dimensional tracking of heterogeneous mammalian chromatin dynamics. Molecular Biology of the Cell, 33(6), ar47.

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Cumate-Induced Fluorescence Characterization

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β-CTF/C6 cells emit endogenous fluorescence after activation by Cumate (System Biosciences, QM100A-1). For analysis, 5 × 10⁵ cells were washed in 500 μL PBS and centrifuged at 1500 rpm for 5 min. The supernatant was discarded, and the centrifugation was repeated once more. The centrifuged cells were resuspended in 500 μL PBS and evaluated using a flow cytometer (Beckman, Coulter CytoFLEX S Flow Cytometry, Brea, CA, USA).

Wuli W., Lin S.Z., Chen S.P., Tannous B.A., Huang W.S., Woon P.Y., Wu Y.C., Yang H.H., Chen Y.C., Fleming R.L., Rogers J.T., Cahill C.M., Ho T.J., Chiou T.W, & Harn H.J. (2022). Targeting PSEN1 by lnc-CYP3A43-2/miR-29b-2-5p to Reduce β Amyloid Plaque Formation and Improve Cognition Function. International Journal of Molecular Sciences, 23(18), 10554.

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Overexpression of miR-155 in C2C12 Myoblasts

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The mouse myoblast cell line C2C12 was cultured in DMEM (Wako, Tokyo, Japan) supplemented with 200 mM L-glutamine and 10% fetal bovine serum (Hyclone, Logan, UT, USA), in 5% CO₂ at 37°C. For overexpression of miR-155, C2C12 cells at 80% confluency were transfected with miExpress EGFP-mmu-miR-155 plasmid (GeneCopoeia Inc. Rockville, MD, USA) or pPBQM-mmu-miR-155 plasmid (a gift from Dr. Martin Lotz) using ScreenFect A (Wako). We also used a scrambled control sequence expression plasmid (CmiR0001-MR04, GeneCopoeia, Inc.) and a mmu-miR-155 precursor expression plasmid (MmiR3427-MR04, GeneCopoeia, Inc.). The cumate-gene switch was activated by adding 30 μg/mL cumate (QM100A-1, System Bioscience Inc., Palo Alto, CA, USA). Myogenic differentiation was induced by culturing confluent C2C12 cells in DMEM containing 2% horse serum (Biowest USA, NW, USA) for 12 days.

Onodera Y., Teramura T., Takehara T., Itokazu M., Mori T, & Fukuda K. (2018). Inflammation-associated miR-155 activates differentiation of muscular satellite cells. PLoS ONE, 13(10), e0204860.

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Inducible Wnt Pathway Modulation

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CHIR 99021 (STEMCELL Technologies #72052) was resuspended in DMSO according to supplied manufacturer recommendations and diluted to 5× concentrated stocks in culture medium immediately prior to use on cells. In all cases, CHIR was used at 10 μM. Doxycycline hyclate (Sigma Aldrich #D9891-1G) was resuspended in phosphate-buffered saline (PBS) and diluted to 5× desired concentration in culture medium prior to use. Stock cumate solution (System Biosciences #QM100A-1) was diluted to 5× in culture medium prior to use.
The “Low” dose of Dox referred to in Fig. 2 C and D in the context of Axin and APC induction was 20 ng/mL concentration in culture medium, “High” dose was 200 ng/mL. “Low” dose of Cumate was 100 ng/mL, and “High” was 1mg/mL. The dose of Dox used in β-cat induction in SI Appendix, Fig. S4 I and J was 100 ng/mL.

Lach R.S., Qiu C., Kajbaf E.Z., Baxter N., Han D., Wang A., Lock H., Chirikian O., Pruitt B, & Wilson M.Z. (2022). Nucleation of the destruction complex on the centrosome accelerates degradation of β-catenin and regulates Wnt signal transmission. Proceedings of the National Academy of Sciences of the United States of America, 119(36), e2204688119.

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