The iPSCs and 10-day differentiation iPGCs were treated with Accutase (Invitrogen), rinsed with culture medium to assist in washing out cells, and pipetted gently up and down. Following a single wash with DPBS containing FBS and 0.1% BSA, the cell suspension was filtered through a 70 μm cell strainer (BD Biosciences) to remove cell aggregates and centrifuged. Cells were treated with the anti-c-KIT antibody (#562,094, BD Biosciences) for 15 min on ice. Nonbinding isotype-matched FITC-conjugated mouse IgG was used in control assays. The cells were suspended in FACS buffer (0.1% BSA in DPBS) and examined using a FACSCalibur Flow Cytometer (BD Biosciences, USA).
Anti c kit antibody
Manufactured by BD
The Anti-c-KIT antibody is a reagent used in laboratory research applications. It is designed to specifically bind and detect the c-KIT protein, which is a receptor tyrosine kinase involved in various cellular processes. The antibody can be utilized in techniques such as Western blotting, immunohistochemistry, and flow cytometry to identify and study the expression of c-KIT in samples.
Automatically generated - may contain errors
2 protocols using anti c kit antibody
1
c-KIT Antibody Staining of iPSCs and iPGCs
2
c-KIT Expression Analysis in iPSCs
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The iPSCs and 10-day differentiation iPGCs were treated with Accutase (Invitrogen), then washed added culture medium to assist in washing off cells, and pipette gently up and down. After washing once with DPBS containing FBS and 0.1% BSA, the cell suspension was ltered by using a cell strainer (BD Biosciences) to remove cell clumps, and was subjected to centrifugation. Cells were incubated with the anti-c-KIT antibody (#562094, BD Biosciences) on ice for 15 min. The nonbinding isotype-matched FITCconjugated mouse IgG was utilized in control experiments. The collected cells were suspended in FACS buffer (0.1% BSA in DPBS) and analyzed by FACSCalibur Flow Cytometer (BD Biosciences, USA).
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