Fibrillar collagen (Horm suspension) was from Nycomed (Munich, Germany). Alexa Fluor® 568 conjugated annexin V (annexin-V; anxV) and Fluo-4 AM, were purchased from Life Technologies. Alexa Fluor® 568-Labelled Lactadherin was purchased from Cambridge Bioscience Ltd. Bovine serum albumin (BSA), ADP, Aspirin and Apyrase were from Sigma Aldrich. RWJ-56110, MRS 2279 and AR-C66096 were from Tocris (Bristol, UK). PPACK was from Enzo life science. Connexin inhibitors, Cx37Gap27 (SRPTEKTIFII) was from Sigma Aldrich and Cx40Gap27 (SRPTEKNVFIV) was from Anaspec, USA. Glass bottom 35 mm dishes (P35G-1.5-20-C) were obtained from MatTek Corporation (USA). Flow chambers (Vena8TM biochip) were from Cellix Ltd. The fluorogenic thrombin substrate Z-Gly-Gly-Arg aminomethyl coumarin (Z-GGR-AMC) was from Bachem AG. Dylight 488–conjugated anti-GPIbβ antibody was from Emfret Analytics, Eibelstadt, Germany.
Z gly gly arg aminomethyl coumarin z ggr amc
Manufactured by Bachem
Sourced in Switzerland
Z-Gly-Gly-Arg aminomethyl coumarin (Z-GGR-AMC) is a synthetic peptide substrate used in biochemical assays. It consists of the amino acid sequence Z-Gly-Gly-Arg linked to the fluorescent coumarin group. The cleavage of the peptide bond by enzymes that recognize the Arg residue results in the release of the coumarin moiety, which can be detected through fluorescence measurement.
Automatically generated - may contain errors
Lab products found in correlation
Fluo 4 am, by Thermo Fisher Scientific (1 mentions)
Apyrase, by Merck Group (1 mentions)
Aspirin, by Merck Group (1 mentions)
Ppack, by Enzo Life Sciences (1 mentions)
Ar c66096, by Bio-Techne (1 mentions)
Mrs2279, by Bio-Techne (1 mentions)
Rwj 56110, by Bio-Techne (1 mentions)
Sulfatide, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
2 protocols using z gly gly arg aminomethyl coumarin z ggr amc
1
Platelets Adhesion and Activation Assay
2
Activating Clotting Cascade In Vitro
Check if the same lab product or an alternative is used in the 5 most similar protocols
CTI was from Haematologic Technologies (Essex Junction, Vermont, United States); recombinant TF Innovin was from Dade Behring (Marburg, Germany); sulfatides and bovine serum albumin were from Sigma (St. Louis, Missouri, United States). Purified human FXII and FXI were obtained from Enzyme Research Laboratories (Stago, Leiden, the Netherlands). Recombinant tissue-type plasminogen activator (tPA) was from Boehringer Ingelheim (Alkmaar, the Netherlands). Synthetic phospholipids DOPS, DOPC, and DOPE (1,2-dioleoyl-
sn-glycero-3-phosphoserine, 1,2-dioleoyl-
sn-glycero-3-choline, and 1,2-dioleoyl-
sn-glycero-3-ethanolamine) were from Avanti Polar Lipids (Alabaster, Alabama, United States). Phospholipid vesicles (DOPS/DOPC/DOPE, 20/60/20 mol/mol/mol) were prepared by sonication, as described earlier.
24 (link)
Fluorogenic thrombin substrate, Z-Gly-Gly-Arg-aminomethyl coumarin (Z-GGR-AMC), was from Bachem (Bubendorf, Switzerland).
+ Expand
CTI was from Haematologic Technologies (Essex Junction, Vermont, United States); recombinant TF Innovin was from Dade Behring (Marburg, Germany); sulfatides and bovine serum albumin were from Sigma (St. Louis, Missouri, United States). Purified human FXII and FXI were obtained from Enzyme Research Laboratories (Stago, Leiden, the Netherlands). Recombinant tissue-type plasminogen activator (tPA) was from Boehringer Ingelheim (Alkmaar, the Netherlands). Synthetic phospholipids DOPS, DOPC, and DOPE (1,2-dioleoyl-
sn-glycero-3-phosphoserine, 1,2-dioleoyl-
sn-glycero-3-choline, and 1,2-dioleoyl-
sn-glycero-3-ethanolamine) were from Avanti Polar Lipids (Alabaster, Alabama, United States). Phospholipid vesicles (DOPS/DOPC/DOPE, 20/60/20 mol/mol/mol) were prepared by sonication, as described earlier.
24 (link)
Fluorogenic thrombin substrate, Z-Gly-Gly-Arg-aminomethyl coumarin (Z-GGR-AMC), was from Bachem (Bubendorf, Switzerland).
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