L-lactic acid (CAS: 79-3344, 129-02666), sodium chloride (CAS: 7647-14-5, 195-15975), potassium dihydrogen phosphate (CAS: 7778-77-0,164-22635), disodium hydrogen phosphate (CAS: 7558-79-4, 042-30055), urea (CAS: 57-13-6, 215-00172), ethanol (CAS: 64-17-5, 057-00456), D-glucose (CAS: 50-99-7, 049-31165), L-valine (CAS: 72-18-4, 228-00082), L-leucine (CAS: 61-90-5, 124-00852), and ammonia (CAS: 1336-21-6, 013-17505) were all purchased from Wako, Japan. Lactate oxidase (LOD) from Aerococcus viridans (T-47, Asahi Kasei Pharma, Tokyo, Japan) was purchased from Asahi Kasei Pharma and was used to modify the electrode of the biosensor. Osmium-wired horseradish peroxidase (002096, Bioanalytical Systems, West Lafayette, IN, USA) was purchased from BAS Inc., Tokyo, Japan. Phosphate-buffered saline (PBS, pH 7.4, 50 mM (PO4)) was prepared in the laboratory. Ultrapure water obtained from the water purification system (Direct-Q, Merck, Darmstadt, Germany) was used for the preparation of all aqueous solutions.
L lactic acid
Manufactured by Fujifilm
Sourced in Japan
L-lactic acid is a chemical compound used in various laboratory applications. It functions as a pH regulator, maintaining the acidity or basicity of solutions. The product is available in different concentrations to accommodate diverse laboratory needs.
Automatically generated - may contain errors
Lab products found in correlation
Direct q, by Merck Group (1 mentions)
Ethanol, by Fujifilm (1 mentions)
Sodium chloride (nacl), by Fujifilm (1 mentions)
D glucose, by Fujifilm (1 mentions)
Urea, by Fujifilm (1 mentions)
Disodium hydrogen phosphate, by Fujifilm (1 mentions)
Potassium dihydrogen phosphate, by Fujifilm (1 mentions)
L valine, by Fujifilm (1 mentions)
L leucine, by Fujifilm (1 mentions)
Stemfit media, by Ajinomoto (1 mentions)
Toluene, by Fujifilm (1 mentions)
Acetic acid, by Fujifilm (1 mentions)
Hydrochloric acid (hcl), by Fujifilm (1 mentions)
Acrylic acid, by Fujifilm (1 mentions)
Ammonium peroxodisulfate, by Fujifilm (1 mentions)
3 aminopropyltrimethoxysilane, by Tokyo Chemical Industry (1 mentions)
Phenol, by Fujifilm (1 mentions)
Tetraethyl orthosilicate, by Fujifilm (1 mentions)
Sodium acetate, by Nacalai Tesque (1 mentions)
Acryloyl chloride, by Fujifilm (1 mentions)
7 protocols using l lactic acid
1
Electrochemical Biosensor for Lactic Acid
2
Cardiac Spheroids from hiPSC-derived Cardiomyocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
hiPSCs (253G4) were maintained on growth factor-reduced Matrigel-coated culture plates using modified Stem Fit media (Ajinomoto, Tokyo, Japan), as reported previously (7 ). hiPSCs were differentiated into CMs and non-CMs using 4-layer culture plates with active gas ventilation (7 ). After cardiac differentiation, the CMs were metabolically selected using glucose- and glutamine-depleted Dulbecco’s modified Eagle’s medium supplemented with 4 mM L-lactic acid (Wako Pure Chemical Industries, Tokyo Japan) (7 , 8 (link), 9 (link)). Differentiation efficiency and purity were evaluated by flow cytometry analysis of cardiac troponin T levels (troponin T antibody [clone: REA400, Miltenyi Biotec, Auburn, California]). CSs were constituted with pure CMs in microwell plates as previously reported (12 (link)). One CS contains ∼1,000 CMs, and its size is ∼200 μm. Contraction and relaxation patterning of CSs were detected and visualized by Cell Motion Imaging System SI8000 (Sony Imaging Products & Solutions Inc., Tokyo, Japan). CSs were stimulated by 0.5-μM isoproterenol, and beating rate was measured.
3
Preparation of Functionalized Porous α-Alumina
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The porous α-alumina porous support (o.d. of 1.9–2.3 mm, average pore diameter of about 150 nm) was supplied by AIST (Japan). Tetraethyl orthosilicate (TEOS; >95.0%), HCl (35%), acryloyl chloride (>98.0%), acrylic acid (>98.0%), ammonium peroxodisulfate (APS; >98.0%), toluene (>95%), tetrahydrofuran (THF; >99.5%), acetic acid (>99.7%), L-lactic acid (85.0%–92.0%), phenol (>99.0%), 0.2 mol/L HCl, and 0.2 mol/L sodium hydroxide aqueous solution were purchased from FUJIFILM Wako Pure Chemical Corp., Osaka, Japan. 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (>95.0%), ethanol (>99.5%), methanol (>99.8%), sodium tetraborate decahydrate (99.5–101.0%), disodium hydrogen phosphate dodecahydrate (>99.0%), and sodium dihydrogen phosphate dihydrate (99.0–102.0%) were purchased from Kanto Chemical Co., Ltd., Tokyo, Japan. 3-Aminopropyltrimethoxysilane (APTMS; >96.0%) was purchased from Tokyo Chemical Industry Co., Ltd., Tokyo, Japan. Sodium acetate (>98.0%) was purchased from Nacalai Tesque Inc., Kyoto, Japan. All chemicals were used without purification. Water was obtained from a water purification system (WG-204, Yamato Co., Tokyo, Japan).
4
3D Sphere Formation Assay in Hypoxia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were plated in ultralow attachment 96-well plates (Corning, Corning, NY, USA) at a density of 100 (normoxia) or 1000 (hypoxia) cells per well, and they were cultured in ULGM or ULGM supplemented with alternative substrates, as indicated. The outermost wells of each plate were excluded from analysis to avoid artifacts due to medium evaporation. Images were acquired with a Cell3 iMager Duos (SCREEN Holdings, Kyoto, Japan) or BZ-X710 (Keyence, Osaka, Japan) inverted microscope at 7 days after plating. Sphere area was quantified with Cell3 iMager Duos Software v1.4 (SCREEN Holdings, Kyoto, Japan) or ImageJ software ver1.51s (NIH, Bethesda, MD, USA). For alternative substrate supplementation, L-lactic acid was purchased from Wako (Osaka, Japan), and all other chemicals from Tokyo Chemical Industry (Tokyo, Japan).
5
Preparation of APA-22-Based Thin Films
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
APA-22 (AMIDET ® APA-22; purity before neutralization ≥ 97％) was supplied by Kao Chemicals Europe, S.L., and used as received. C 18 OH (KALCOL 8098; purity ≥ 98％) was obtained from Kao Corporation and used without further purification. L-lactic acid was purchased from Fujifilm Wako Chemical Corporation and used without further purification. The chemical structures of APA-22 and L-lactic acid are shown in Fig. 1. Flat silica plates were prepared from silicon wafers (Nilaco) via chemical oxidation in a mixture of H 2 O/NH 3 /H 2 O 2 at a 5:1:1 ratio (by volume) . The detailed procedure has been previously reported 18) . The water used in this study was purified using a Millipore Direct-Q UV 3 water purification system.
6
Preparation of Cationic Lipid Dispersions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
APA-22 (AMIDET ® APA-22; purity before neutralization ≥ 97％) was supplied by Kao Chemicals Europe, S.L., and used as received. C 18 OH (KALCOL 8098; purity ≥ 98％) was obtained from Kao Corporation and used without further purification. L-lactic acid was purchased from Fujifilm Wako Chemical Corporation and used without further purification. 1,1 -Dioctadecyl-3,3,3 ,3 -tetramethylindocarbocyanine perchlorate (DiI; purity ≥ 97％) was supplied by Sigma-Aldrich and used as received. Water used in this study was deionized using an ion exchange resin cartridge (Yamato CPC-S, 4L) and filtered through a membrane filter (pore size＝0.10 μm) .
7
Synthesis of Long-Chain Alcohol Surfactant
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
2.1 Materials APA-22 (AMIDET ® APA-22) was supplied by Kao Chemicals Europe S.L. and used unprocessed. According to the information provided by the supplier, its purity (before neutralization) exceeded 97％. Long-chain alcohol 1-octadecanol (C 18 OH, KALCOL 8098) was obtained from Kao Corporation and used without further purification. The purity of C 18 OH exceeded 98％. L-Lactic acid was purchased from FUJIFILM Wako Chemical Corporation and used without purification. The water used in this study was deionized with an ion exchange resin cartridge (Yamato, CPC-S, 4L) and filtered with a membrane filter (pore size 0.10 μm) .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!