TEM images were taken with a FEI L120 operating at 120 kV. Agarose gel electrophoresis was executed in a water-cooled CBS Scientific HSU-020 gel electrophoresis chamber using an Enduro 300 V power source. Gel imaging was done with an INTAS ECL Chemostar. DNA origami were folded in a Biometra TPersonal Thermocycler. Nanotubes were incubated in an Eppendorf ThermoMixer C. UV-Vis measurements were conducted on an AnalytikJena ScanDrop 250 using a Tray cell cuvette from Hellma with a path length of 1 mm or a Hellma microcuvette with a path length of 3 mm. Fluorescence spectroscopy was done with the Tecan Spark plate reader in top mode.
Scandrop 250
Manufactured by Hellma
The ScanDrop 250 is a compact, UV-Vis spectrophotometer designed for accurate measurements of sample absorbance. It features a 2.8-inch color touchscreen display, a wavelength range of 190-1100 nm, and a photometric range of -3 to 3 Abs. The ScanDrop 250 is suitable for a variety of applications in research and laboratory settings.
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2 protocols using scandrop 250
1
Multimodal Characterization of DNA Origami
2
DNA Origami Concentration Measurement
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Ultraviolet (UV)–visible measurements were conducted on an AnalytikJena ScanDrop 250 using a Hellma TrayCell with a path length of 1 mm. DNA concentrations were calculated using the absorption at 260 nM and the calculated extinction coefficient for the respective DNA strand. DNA origami concentrations were measured using the estimated conversion of absorbance A260 of dsDNA = 50 μg ml−1.
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