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Mm01302388 m1

Manufactured by Thermo Fisher Scientific
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The Mm01302388_m1 is a laboratory instrument designed for scientific research applications. It is a precision tool that can be used to perform various analytical and experimental tasks within a controlled laboratory environment. The core function of this product is to provide reliable and accurate measurements or analysis, as required by the user's research objectives. However, a more detailed description cannot be provided while maintaining an unbiased and factual approach, as requested.

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Lab products found in correlation

Rna storage buffer, by Thermo Fisher Scientific (2 mentions) 7900ht fas real time pcr system, by Thermo Fisher Scientific (2 mentions)

3 protocols using mm01302388 m1

1

Quantification of B Cell Gene Expression

Cited 1 time
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Total RNA from different B cell subsets was isolated with RNeasy (Qiagen) and stored at −80C in RNA storage buffer (Ambion) until use. Complementary DNA was prepared as previously described20 . Rag1, Aicda, Cdkn1b, Bcl2l11 and membrane IgM mRNA expression were measured as described previously29 (link), 51 (link). Fcmr expression was measured using a commercial primer/probe set (Mm01302388_m1; Applied Biosystems). Quantitative PCR was performed using a 7900HT Fas Real-Time PCR system (Applied Biosystems) with following cycles: 50°C for 2 min, 95°C for 10 min, and 40 cycles of 95°C for 15s and 60°C for 1 min. Relative expression was normalized to Gapdh (Mm.P.T.39a.1; IDT).
Nguyen T.T., Kläsener K., Zürn C., Castillo P.A., Brust-Mascher I., Imai D.M., Bevins C.L., Reardon C., Reth M, & Baumgarth N. (2017). The FcμR Limits Tonic BCR Signaling by Regulating IgM-BCR Expression. Nature immunology, 18(3), 321-333.
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2

Quantification of B Cell Gene Expression

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA from different B cell subsets was isolated with RNeasy (Qiagen) and stored at −80C in RNA storage buffer (Ambion) until use. Complementary DNA was prepared as previously described20 . Rag1, Aicda, Cdkn1b, Bcl2l11 and membrane IgM mRNA expression were measured as described previously29 (link), 51 (link). Fcmr expression was measured using a commercial primer/probe set (Mm01302388_m1; Applied Biosystems). Quantitative PCR was performed using a 7900HT Fas Real-Time PCR system (Applied Biosystems) with following cycles: 50°C for 2 min, 95°C for 10 min, and 40 cycles of 95°C for 15s and 60°C for 1 min. Relative expression was normalized to Gapdh (Mm.P.T.39a.1; IDT).
Nguyen T.T., Kläsener K., Zürn C., Castillo P.A., Brust-Mascher I., Imai D.M., Bevins C.L., Reardon C., Reth M, & Baumgarth N. (2017). The FcμR Limits Tonic BCR Signaling by Regulating IgM-BCR Expression. Nature immunology, 18(3), 321-333.
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3

Quantifying B Cell Fcmr mRNA

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Total RNA from different B cell subsets was isolated as previously described (32 ). Fcmr mRNA expression was measured using a commercial primer/probe set (Mm01302388_m1; Applied Biosystems). Relative expression was normalized to Ubc (Applied Biosystems).
Nguyen T.T., Graf B.A., Randall T.D, & Baumgarth N. (2017). sIgM - FcμR interactions regulate early B cell activation and plasma cell development after influenza virus infection. Journal of immunology (Baltimore, Md. : 1950), 199(5), 1635-1646.
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